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EMAGE:15701

Synpo synaptopodin ( MGI:1099446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701
"Pseudo-wholemount" of euxassay_010928. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010928_01 euxassay_010928_02 euxassay_010928_03 euxassay_010928_04
EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701
euxassay_010928_05 euxassay_010928_06 euxassay_010928_07 euxassay_010928_08 euxassay_010928_09
EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701
euxassay_010928_10 euxassay_010928_11 euxassay_010928_12 euxassay_010928_13 euxassay_010928_14
EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701 EMAGE:15701
euxassay_010928_15 euxassay_010928_16 euxassay_010928_17 euxassay_010928_18 euxassay_010928_19
EMAGE:15701
euxassay_010928_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15701Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15701_wholemount_strong.wlz
15701_wholemount_moderate.wlz
15701_wholemount_weak.wlz
15701_wholemount_possible.wlz
15701_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15701_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 06 07 08 09 10 15 moderate expression: see section 05 16 17 18 19
humerus
moderate moderate
regionalmoderate expression: see section 03 18 19
forelimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 04 07 08
forelimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 04 07 08 09
forelimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 03 04 07 08
fibula
moderate moderate
regionalmoderate expression: see section 02 03
tibia
moderate moderate
regionalmoderate expression: see section 02 03
femur
moderate moderate
regionalmoderate expression: see section 06 14 16 19
otic capsule
strong strong
regionalstrong expression: see section 07 14 15 16 moderate expression: see section 05 06
naris
strong strong
regionalstrong expression: see section 11 12 13 14 15
nasal septum
strong strong
regionalstrong expression: see section 13
viscerocranium
strong strong
regionalExpression in the turbinate bone.
aorta
strong strong
regionalstrong expression: see section 07 09
heart valve
strong strong
regionalstrong expression: see section 08 09 10 11 12
lower jaw incisor
strong strong
regionalstrong expression: see section 11 12
lower jaw molar
strong strong
regionalstrong expression: see section 06 17 18
upper jaw incisor
strong strong
regionalstrong expression: see section 11
upper jaw molar
strong strong
regionalstrong expression: see section 06 17 18
larynx
strong strong
regionalstrong expression: see section 11
trachea cartilaginous ring
strong strong
regionalstrong expression: see section 11
axial skeleton
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 moderate expression: see section 05 14
basioccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 03 04 09 10 11 12 15 16 17 18 19 20
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 09 10 11 12
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 17 18 19
vault of skull
moderate moderate
regionalmoderate expression: see section 01 02
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 16 17 18 19 20
sternum
strong strong
regionalstrong expression: see section 11
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39011
Entity Detected:Synpo, synaptopodin ( MGI:1099446)
Sequence:sense strand is shown

>T39011
ACCTAGTGCAGACAGCTGATGAGAAGCGGAGGCAGAGAGACCACGGGGAGGTGGGCATGGAAGAAGAGCC
CTTTGCCCTGGGAGCTGAGGCCTCCAACTTCCAGCAGGAGCCAATAGCTCGGGACAGGGCCAGCCCTGCG
GCAGCTGAGGAAGCTGTCCCTGAGTGGGCCTCTTGCCTCAAGTCACCCCGTATCCAGGCCAAGCCGAAGC
CCAAACCCAATCAGAACCTCTCGGAAGCCTCAGGGAAGGGGGCTGAGCTCTATGCCCGCCGCCAGTCACG
GATGGAGAAATACGTCATAGAGTCTTCAGGCCATGCCGAATTGGCCCGCTGCCCTTCACCTACCATGTCC
CTGCCTTCATCCTGGAAGTACACCACTAACGCCCCTGGGGGCTTCCGAGTGGCATCCTTAAGTCCAGCGC
GGACCCCGCCTGCCTCTCTCTACCACGGCTATCTGCCAGAGAATGGAGTCCTGCGCCCTGAACCTACCAA
GCAGCAGCCATACCAGATGCGGCCTTCCCTCTATGCTCTGTCGCCCGTCAAGGAACCTGCCAAGGCCTCA
TCGCGTGCCACCTCATCGCGCACTCCATCACGCACTGTCTCACCTCGAGCTGCCTCCCCGGCCAAGCCTA
GCTCTCTGGACCTGGTGCCCAACCTGCCCAGAGCAGGCCTCCCACCATCTCCTGCTCTGCCTCGGCCTTC
CCGCTCCTCCCCAGGCCTCTACACTGCCCCAGTCCAAGACAGCCTCCAGCCCACTGCCGTGAGTCCCACT
TACAGCAGTGATATCTCCCCCGTGTCTCCCTCCAGGGCGTGGTCTCCTCGAGCCAAGCAGGCTCCCAGGC
CTTCCTTCTCCACCCGGAATGCTGGGATCGAGGCCCAGGTGTGGAAGCCCTCCTTCTGCTTCAAGTAACA
CATCTCAGGGCCTGTCTTGTCCCCTTCTCGGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 267407. Forward Primer - name:267407_F_cDNA_Synpo, sequence:ACCTAGTGCAGACAGCTGATGA; Reverse Primer - name:267407_N_SP6_cDNA_Synpo, sequence:CTCCGAGAAGGGGACAAGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15704 same embryo
 EMAGE:15702 same embryo
 EMAGE:15703 same embryo
 EMAGE:15699 same embryo
 EMAGE:15700 same embryo
 EurExpress:euxassay_010928 same experiment
 MGI:4828560 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS