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EMAGE:15796

LOC671232 (LOC671232)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796
"Pseudo-wholemount" of euxassay_013232. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013232_01 euxassay_013232_02 euxassay_013232_03 euxassay_013232_04
EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796
euxassay_013232_05 euxassay_013232_06 euxassay_013232_07 euxassay_013232_08 euxassay_013232_09
EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796
euxassay_013232_10 euxassay_013232_11 euxassay_013232_12 euxassay_013232_13 euxassay_013232_14
EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796
euxassay_013232_15 euxassay_013232_16 euxassay_013232_17 euxassay_013232_18 euxassay_013232_19
EMAGE:15796 EMAGE:15796 EMAGE:15796 EMAGE:15796
euxassay_013232_20 euxassay_013232_21 euxassay_013232_22 euxassay_013232_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15796Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15796_wholemount_strong.wlz
15796_wholemount_moderate.wlz
15796_wholemount_weak.wlz
15796_wholemount_possible.wlz
15796_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15796_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 14 15 16
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 11 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39556
Entity Detected:LOC671232, (LOC671232)
Sequence:sense strand is shown

>T39556
ACTTAGCCCAGGGATCTTGTTTCAGTGAATAATGTCTCTCAGAACACGGGTTGTCTGAAGCTTTGTGTTT
TCAGTTACAACCTAGAGAGTTTAGAGCCTCAAGAGTTCTTTGTTACTTGAAATAGAGGGAGTTAGAACAC
TTGGTGATTTCATCTGCTAAACCTGCTGCAGAGCCATAACTGAGAAGTTCTGAAGTGGACCATCGTGTCC
AGGATCTGGAAGTCCTTGACCTGAACTTCATGCTGCACAAGATAATGTCAGTATTGTACATTTCATGGTA
TGAAATATGAAGCACCCATTGCAGAGCCTTATTACCTAATACACACCCTTTCAGAAATTCAGCGTGTATT
CACATAGACCATAAGAGGAATTACAGGTTGATGGAAATTTTTAAAAGTAGATTTGAGTATTGTGTATTTT
GTTTATTTACAAATACGTAGACTTTACTACTTACATATGCAGGACAGTTGACAGTGACGTCATACTTGTT
TACGAGAGGTAAACTTTTTAGTTGTACAAGTGTACAGTTGTCACACGTGCCATAGAGTGCTACACTGAGA
GTCCTGGTGCCCTGTCTAGACCATCACCACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108537. Forward Primer - name:108537_F_cDNA_LOC233637, sequence:ACTTAGCCCAGGGATCTTGTTT; Reverse Primer - name:108537_N_SP6_cDNA_LOC233637, sequence:CTGTGGTGATGGTCTAGACAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15797 same embryo
 EMAGE:15799 same embryo
 EMAGE:15795 same embryo
 EMAGE:15798 same embryo
 EMAGE:15794 same embryo
 EurExpress:euxassay_013232 same experiment
 MGI:4829026 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS