EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16062

Pls1 plastin 1 (I-isoform) ( MGI:104809)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013517_01	euxassay_013517_02	euxassay_013517_03	euxassay_013517_04

euxassay_013517_05	euxassay_013517_06	euxassay_013517_07	euxassay_013517_08	euxassay_013517_09

euxassay_013517_10	euxassay_013517_11	euxassay_013517_12	euxassay_013517_13	euxassay_013517_14

euxassay_013517_15	euxassay_013517_16	euxassay_013517_17	euxassay_013517_18	euxassay_013517_19

euxassay_013517_20	euxassay_013517_21	euxassay_013517_22	euxassay_013517_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16062_wholemount_strong.wlz

16062_wholemount_moderate.wlz

16062_wholemount_weak.wlz

16062_wholemount_possible.wlz

16062_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16062_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45003
Entity Detected:	Pls1, plastin 1 (I-isoform) ( MGI:104809)
Sequence:	sense strand is shown >T45003 TAACTCTTGAATAACCCCCACGTTCACTCTCTTGTTTAAGCTGTAGGAGATTTTTACTTACAGTTTGTTT GTAGCTCCATTGTTACTTCACATGTGCTAGTCTGATAACACAGCTTTGTCTCCAGCATCACATGTGCTAG TCTGATAACACAGCTTTGTCTCCACCATCAAGCATAATACTATATGTTGGTAGGATGTTACACATATGTA TTTCTCTAAGTCATCTGAGTTACCCACCTTTTCTAAAATGGTATGTATTAAGTGGTCAGCCAGCCGTTAA GTATTTATTTTGCCCCCACAATGTGTGTGACACTTTTCAATTGTGGATAATCTGATCTGTGGAGATCTGT TTTGCAAATATTAATTCTGAATGTGAATATCGTTATTTTTCACCTAACTGCTTTCAAATACTTTTCAGCC AAACATTGGATAGAATGTCCTAACTGACACCAATCCTATCCTGCTAGAATCACATCACACTTACTACTCT CAGGCTACAGGGAAACCTACATCACTGGACATGCCCACCAAATGGCTTCTGTAGAGATCTGTGTATATAA AAGCTAATGTCCGTCGTAGTTTTTCATGGTCTCCTGGAAACAGTTGAGGTTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 204182. Forward Primer - name:204182_F_exon_AI427122, sequence:TAACTCTTGAATAACCCCCACG; Reverse Primer - name:204182_N_SP6_exon_AI427122, sequence:CAAACCTCAACTGTTTCCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16061 same embryo
	EMAGE:16063 same embryo
	EMAGE:16066 same embryo
	EMAGE:16065 same embryo
	EMAGE:16064 same embryo
	EurExpress:euxassay_013517 same experiment
	MGI:4827284 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source