EMAGE mouse gene expression spatial database

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EMAGE:16105

Robo3 roundabout homolog 3 (Drosophila) ( MGI:1343102)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013562. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013562_01	euxassay_013562_02	euxassay_013562_03	euxassay_013562_04

euxassay_013562_05	euxassay_013562_06	euxassay_013562_07	euxassay_013562_08	euxassay_013562_09

euxassay_013562_10	euxassay_013562_11	euxassay_013562_12	euxassay_013562_13	euxassay_013562_14

euxassay_013562_15	euxassay_013562_16	euxassay_013562_17	euxassay_013562_18	euxassay_013562_19

euxassay_013562_20	euxassay_013562_21	euxassay_013562_22	euxassay_013562_23	euxassay_013562_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16105_wholemount_strong.wlz

16105_wholemount_moderate.wlz

16105_wholemount_weak.wlz

16105_wholemount_possible.wlz

16105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 13 15 16

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 08 09 11 12 13 14

medulla oblongata alar plate marginal layer

strong

regional

strong expression: see section 06 07 15 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09

medulla oblongata basal plate marginal layer

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 04 05 19 20 21

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 13 14 15 16 17 18 19

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 15 16 19 20 21

midbrain mantle layer

strong

regional

strong expression: see section 07 08 09 17 18

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45167
Entity Detected:	Robo3, roundabout homolog 3 (Drosophila) ( MGI:1343102)
Sequence:	sense strand is shown >T45167 CTTGCAGAAGTCCCAATAGTCCCCAGCTTCCATTGGATTCATGCATCTGGTCAACATTGAAATTGTCTCT TGTCTCTTTCATTCCCTCTAAGTAAAAGTTCTCAGAGTCTCCACAATCCCCCCCCCCCCCCTTCCAGGAC TGAAGCCAAGGATGAGGAAAAAGTAGTGTCTGGGTGCTCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 228017. Forward Primer - name:228017_F_exon_Robo3, sequence:CTTGCAGAAGTCCCAATAGTCC; Reverse Primer - name:228017_N_SP6_exon_Robo3, sequence:CAGAGCACCCAGACACTACTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16103 same embryo
	EMAGE:16106 same embryo
	EMAGE:16107 same embryo
	EMAGE:16104 same embryo
	EMAGE:16108 same embryo
	EurExpress:euxassay_013562 same experiment
	MGI:4827786 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source