EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16403

Mir324 microRNA 324 ( MGI:3619335)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019156. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019156_01	euxassay_019156_02	euxassay_019156_03	euxassay_019156_04

euxassay_019156_05	euxassay_019156_06	euxassay_019156_07	euxassay_019156_08	euxassay_019156_09

euxassay_019156_10	euxassay_019156_11	euxassay_019156_12	euxassay_019156_13	euxassay_019156_14

euxassay_019156_15	euxassay_019156_16	euxassay_019156_17	euxassay_019156_18	euxassay_019156_19

euxassay_019156_20	euxassay_019156_21	euxassay_019156_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16403_wholemount_strong.wlz

16403_wholemount_moderate.wlz

16403_wholemount_weak.wlz

16403_wholemount_possible.wlz

16403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 04 05 06 07 08 11 20 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 weak expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 weak expression: see section 16 17 18 19 20

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70400
Entity Detected:	Mir324, microRNA 324 ( MGI:3619335)
Sequence:	sense strand is shown >T70400 CCACTGCCCCAGGTGCTGCT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-324-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16400 same embryo
	EMAGE:16399 same embryo
	EMAGE:16401 same embryo
	EMAGE:16402 same embryo
	EurExpress:euxassay_019156 same experiment
	MGI:4826269 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source