EMAGE mouse gene expression spatial database

EMAGE:16419

Mir199b microRNA 199b ( MGI:2676865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019074. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019074_01	euxassay_019074_02	euxassay_019074_03	euxassay_019074_04

euxassay_019074_05	euxassay_019074_06	euxassay_019074_07	euxassay_019074_08	euxassay_019074_09

euxassay_019074_10	euxassay_019074_11	euxassay_019074_12	euxassay_019074_13	euxassay_019074_14

euxassay_019074_15	euxassay_019074_16	euxassay_019074_17	euxassay_019074_18	euxassay_019074_19

euxassay_019074_20	euxassay_019074_21

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70316
Entity Detected:	Mir199b, microRNA 199b ( MGI:2676865)
Sequence:	sense strand is shown >T70316 ACAGTAGTCTGCACATTGGTTA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-199b was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16420 same assay
	EMAGE:16421 same assay
	EMAGE:16417 same embryo
	EurExpress:euxassay_019074 same experiment
	EMAGE:16416 same embryo
	MGI:4826237 same experiment
	MGI:4826238 same experiment
	MGI:4826236 same experiment
	EMAGE:16418 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source