EMAGE mouse gene expression spatial database

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EMAGE:16547

Ccz1 CCZ1 vacuolar protein trafficking and biogenesis associated homolog (S. cerevisiae) ( MGI:2141070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008787. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008787_01	euxassay_008787_02	euxassay_008787_03	euxassay_008787_04

euxassay_008787_05	euxassay_008787_06	euxassay_008787_07	euxassay_008787_08	euxassay_008787_09

euxassay_008787_10	euxassay_008787_11	euxassay_008787_12	euxassay_008787_13	euxassay_008787_14

euxassay_008787_15	euxassay_008787_16	euxassay_008787_17	euxassay_008787_18	euxassay_008787_19

euxassay_008787_20	euxassay_008787_21	euxassay_008787_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16547_wholemount_strong.wlz

16547_wholemount_moderate.wlz

16547_wholemount_weak.wlz

16547_wholemount_possible.wlz

16547_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16547_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 19 20 21 22

upper leg muscle

strong

regional

strong expression: see section 06 07 08 19 20 21 22

diaphragm

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21

shoulder rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 22

hand mesenchyme

strong

regional

strong expression: see section 02 03 04 05

foot mesenchyme

strong

regional

strong expression: see section 08 09 10

lower leg rest of mesenchyme

strong

regional

strong expression: see section 06 07

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

adrenal gland

moderate

regional

moderate expression: see section 07 08 14 15

forebrain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

midbrain

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

spinal cord

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12

extrinsic ocular muscle

strong

regional

strong expression: see section 02 03 04 05 06 17 18 19 20 21 22

tongue muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15

lower jaw molar

strong

regional

strong expression: see section 06 07 19

upper jaw incisor

strong

regional

strong expression: see section 10 11 14 15

upper jaw molar

strong

regional

strong expression: see section 06 07 19

metanephros

moderate

regional

moderate expression: see section 07 08 09 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1239
Entity Detected:	Ccz1, CCZ1 vacuolar protein trafficking and biogenesis associated homolog (S. cerevisiae) ( MGI:2141070)
Sequence:	sense strand is shown >T1239 CCTCGAGCCTGTTGGCCTACTGGGATGAAAANTTAGAAATGTTGGATTATGTGAAGCAATTGTACAGTTT ACAAGGACTTTTAGCCCATCGAAACCTGCAAAATCTTTACACACACAGAAGAACAGACAGTTCTTCAATG AACCAGAAGAAAATTTCTGGATGGTTATGGTTGTTCGGAATCCTATCATCGAGAAGCAAAGTAAAGATGG CAAGGCGGTAGTGGAGTACCAAGAGGAGGAGCTGTTGGACAAGGTGTACAGCTCAGTGCTGCAGCAGTGC TACAGCATGTACAAGCTTTTCAATGGGACGTTTCTGAAAGCCATGGAAGATGGAGGCGTCAAGCTCCTAA AGGAAAGATTAGAGAAATTCTTCCATCGGTATTTGCAGACACTACATTTGCAGTCATGTGACCTACTTGA TATTTTTGGTGGAATCAGCTTCTTCCCATTGGATAAAATGACTTACTTGAAAATCCAGTCCTTTATTAAC CGAATGGAAGAAAGCCTAAGTGTAGTCAAATATACAGCATTCCTCTACAATGACCA
Notes:	The probe template was PCR amplified from IMAGE:2192844 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2192844 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16545 same embryo
	EMAGE:16544 same embryo
	EMAGE:16546 same embryo
	EurExpress:euxassay_008787 same experiment
	MGI:4823354 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source