EMAGE mouse gene expression spatial database

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EMAGE:16733

Mir485 microRNA 485 ( MGI:3619422)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019298_01	euxassay_019298_02	euxassay_019298_03	euxassay_019298_04

euxassay_019298_05	euxassay_019298_06	euxassay_019298_07	euxassay_019298_08	euxassay_019298_09

euxassay_019298_10	euxassay_019298_11	euxassay_019298_12	euxassay_019298_13	euxassay_019298_14

euxassay_019298_15	euxassay_019298_16	euxassay_019298_17	euxassay_019298_18	euxassay_019298_19

euxassay_019298_20	euxassay_019298_21	euxassay_019298_22	euxassay_019298_23	euxassay_019298_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16733_wholemount_strong.wlz

16733_wholemount_moderate.wlz

16733_wholemount_weak.wlz

16733_wholemount_possible.wlz

16733_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16733_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 05

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 05

diencephalon

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

telencephalon

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 01 02 03 21 22 23

hindbrain

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 02 03

midbrain

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

moderate

single cell

moderate expression: see section 04 18

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 07 16

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20

spinal cord

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

single cell

moderate expression: see section 06 07 08 09 13 14

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

exoccipital bone

moderate

regional

moderate expression: see section 01 02 21 22 weak expression: see section 03 18 20

temporal bone

moderate

regional

moderate expression: see section 01 02 21 22 weak expression: see section 03 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 02 weak expression: see section 05 06 07 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70058
Entity Detected:	Mir485, microRNA 485 ( MGI:3619422)
Sequence:	sense strand is shown >T70058 AGAGGCTGGCCGTGATGAATTC
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-485 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16734 same embryo
	EurExpress:euxassay_019298 same experiment
	MGI:4826316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source