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EMAGE:16742

Mir503 microRNA 503 ( MGI:3619428)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742
"Pseudo-wholemount" of euxassay_019291. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019291_01 euxassay_019291_02 euxassay_019291_03 euxassay_019291_04
EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742
euxassay_019291_05 euxassay_019291_06 euxassay_019291_07 euxassay_019291_08 euxassay_019291_09
EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742
euxassay_019291_10 euxassay_019291_11 euxassay_019291_12 euxassay_019291_13 euxassay_019291_14
EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742
euxassay_019291_15 euxassay_019291_16 euxassay_019291_17 euxassay_019291_18 euxassay_019291_19
EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742 EMAGE:16742
euxassay_019291_20 euxassay_019291_21 euxassay_019291_22 euxassay_019291_23 euxassay_019291_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16742Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16742_wholemount_strong.wlz
16742_wholemount_moderate.wlz
16742_wholemount_weak.wlz
16742_wholemount_possible.wlz
16742_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16742_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
humerus
moderate moderate
regionalmoderate expression: see section 22 23 24 weak expression: see section 01 02 03 04 21
fibula
weak weak
regionalweak expression: see section 03 04
tibia
weak weak
regionalweak expression: see section 04
femur
moderate moderate
regionalmoderate expression: see section 09 10 11 24 weak expression: see section 05 07 08
nasal septum
moderate moderate
regionalmoderate expression: see section 14
meckel's cartilage
weak weak
regionalweak expression: see section 06 07 08 09 10 14 17 18 20
axial skeleton
weak weak
regionalweak expression: see section 09 10 13 14 15 16 17 18
basioccipital bone
weak weak
regionalweak expression: see section 09 10 11 13 15 16
exoccipital bone
moderate moderate
regionalmoderate expression: see section 02 19 20 weak expression: see section 03 04 05
temporal bone
moderate moderate
regionalmoderate expression: see section 01 02 20 21 22 23 24 weak expression: see section 03 04 05
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 07 08 20 21 24 weak expression: see section 06 09 10 11 13 15 19
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 02 03 22 weak expression: see section 23
pelvic girdle skeleton
weak weak
regionalweak expression: see section 13 14 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70074
Entity Detected:Mir503, microRNA 503 ( MGI:3619428)
Sequence:sense strand is shown

>T70074
TAGCAGCGGGAACAGTACTGCAG
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-503 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16741 same embryo
 EMAGE:16740 same embryo
 EurExpress:euxassay_019291 same experiment
 MGI:4826323 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS