EMAGE mouse gene expression spatial database

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EMAGE:16759

Mir129-1 microRNA 129-1 ( MGI:2676815)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019314. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019314_01	euxassay_019314_02	euxassay_019314_03	euxassay_019314_04

euxassay_019314_05	euxassay_019314_06	euxassay_019314_07	euxassay_019314_08	euxassay_019314_09

euxassay_019314_10	euxassay_019314_11	euxassay_019314_12	euxassay_019314_13	euxassay_019314_14

euxassay_019314_15	euxassay_019314_16	euxassay_019314_17	euxassay_019314_18	euxassay_019314_19

euxassay_019314_20	euxassay_019314_21	euxassay_019314_22	euxassay_019314_23	euxassay_019314_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16759_wholemount_strong.wlz

16759_wholemount_moderate.wlz

16759_wholemount_weak.wlz

16759_wholemount_possible.wlz

16759_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16759_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

single cell

moderate expression: see section 14 15 16 17 18 19 weak expression: see section 09 10 11 12 13

telencephalon

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22 weak expression: see section 09 10 11 12 13 23 24

hindbrain

moderate

single cell

moderate expression: see section 06 07 08 14 15 16 17 18 19 20 21 22 weak expression: see section 09 10 11 12 13

midbrain

moderate

single cell

moderate expression: see section 14 15 16 17 18 19 20 21 22 weak expression: see section 09 10 11 12 13

facial vii ganglion

moderate

single cell

moderate expression: see section 06

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 08

trigeminal v ganglion

moderate

single cell

moderate expression: see section 04 05 06 07 08 17 18 19 20 21

spinal cord

moderate

single cell

moderate expression: see section 14 15 16 weak expression: see section 11 12 13

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 10 17 weak expression: see section 16

neural retina

moderate

regional

moderate expression: see section 02 weak expression: see section 01 03 22 23

mandible

moderate

regional

moderate expression: see section 04 05 06 19 weak expression: see section 07 08 16 17 18 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70253
Entity Detected:	Mir129-1, microRNA 129-1 ( MGI:2676815)
Sequence:	sense strand is shown >T70253 AAGCCCTTACCCCAAAAAGCAT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-129-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16760 same assay
	EMAGE:16755 same embryo
	EMAGE:16756 same embryo
	EMAGE:16758 same embryo
	EurExpress:euxassay_019314 same experiment
	MGI:4826208 same experiment
	MGI:4826209 same experiment
	EMAGE:16757 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source