EMAGE mouse gene expression spatial database

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EMAGE:16787

C1qtnf7 C1q and tumor necrosis factor related protein 7 ( MGI:1925911)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008792. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008792_01	euxassay_008792_02	euxassay_008792_03	euxassay_008792_04

euxassay_008792_05	euxassay_008792_06	euxassay_008792_07	euxassay_008792_08	euxassay_008792_09

euxassay_008792_10	euxassay_008792_11	euxassay_008792_12	euxassay_008792_13	euxassay_008792_14

euxassay_008792_15	euxassay_008792_16	euxassay_008792_17	euxassay_008792_18	euxassay_008792_19

euxassay_008792_20	euxassay_008792_21	euxassay_008792_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16787_wholemount_strong.wlz

16787_wholemount_moderate.wlz

16787_wholemount_weak.wlz

16787_wholemount_possible.wlz

16787_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16787_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

radius

moderate

regional

moderate expression: see section 22

ulna

moderate

regional

moderate expression: see section 22

humerus

weak

regional

weak expression: see section 01 02 03 04 18 19 20

fibula

weak

regional

weak expression: see section 03 19 20

tibia

weak

regional

weak expression: see section 03 19 20

basioccipital bone

moderate

regional

moderate expression: see section 01 02 22

basisphenoid bone

moderate

regional

moderate expression: see section 01 02 22

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 18 19 20 21 22

not examined

regional

not examined expression: see section 18

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 01 03 06 07 18 19 21 weak expression: see section 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 02 03 04 19 20

pelvic girdle skeleton

moderate

regional

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2770
Entity Detected:	C1qtnf7, C1q and tumor necrosis factor related protein 7 ( MGI:1925911)
Sequence:	sense strand is shown >T2770 TTTTCTGGACCCCTGGGCCTTGCTGGTGAGAAAGGAGACCAAGGAGAAACTGGAAAGAAAGGACCCATAG GACCAGAGGGTGAGAAAGGAGAAGTCGGTCCAGCTGGGCCTCCTGGGCCAAAGGGAGACAGAGGAGATCA AGGGGACCCAGGGCTGCCTGGAGTGTGCAGGTGTGGAAGCATTGTGCTCAAATCTGCCTTTTCAGTTGGC ATCACAACCAGCTACCCAGAAGAAAGACTACCCATCATATTTAACAAAGTCCTCTTCAATGAGGGGGAGC ATTACAACCCTGCAACGGGGAAGTTCATTTGCGCTTTCCCAGGGATCTATTACTTTTCTTATGACATCAC GTTGGCCAATAAGCACCTAGCAATCGGGCTGGTGCACAATGGGCAGTACCGGATAAGGACCTTTGATGCC AACACAGGGAACCATGATGTGGCGTCGGGGTCCACAGTCATCTACCTGCAGCCAGAAGATGAGGTCTGGC TGGAGATCTTCTTCAATGACCAGAACGGCCTCTTCTCAGATCCAGG
Notes:	The probe template was PCR amplified from IMAGE:1514873 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514873 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16790 same embryo
	EMAGE:16789 same embryo
	EMAGE:16788 same embryo
	EurExpress:euxassay_008792 same experiment
	MGI:4823542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source