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EMAGE:17171

Enox2 ecto-NOX disulfide-thiol exchanger 2 ( MGI:2384799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171
"Pseudo-wholemount" of euxassay_001555. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001555_01 euxassay_001555_02 euxassay_001555_03 euxassay_001555_04
EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171
euxassay_001555_05 euxassay_001555_06 euxassay_001555_07 euxassay_001555_08 euxassay_001555_09
EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171
euxassay_001555_10 euxassay_001555_11 euxassay_001555_12 euxassay_001555_13 euxassay_001555_14
EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171 EMAGE:17171
euxassay_001555_15 euxassay_001555_16 euxassay_001555_17 euxassay_001555_18 euxassay_001555_19
EMAGE:17171 EMAGE:17171 EMAGE:17171
euxassay_001555_20 euxassay_001555_21 euxassay_001555_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17171Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17171_wholemount_strong.wlz
17171_wholemount_moderate.wlz
17171_wholemount_weak.wlz
17171_wholemount_possible.wlz
17171_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17171_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21
neural retina
weak weak
regionalweak expression: see section 01 02 03 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4770
Entity Detected:Enox2, ecto-NOX disulfide-thiol exchanger 2 ( MGI:2384799)
Sequence:sense strand is shown

>T4770
AGACAGTTGTGGGGCACACCCCACCCTGAGGGCACTCCGGGTCCAGGAACTTCCGAGCGGGTCCGATTTT
CCGCGGCTGTTTAAGGAGTAGCGCTTCGGTAGCCGGGGAGCTGGAGGGGAACCTGATTTGAGATCAATTC
TTGGAGAACACAAAGAAGTGTGGTGGATTGAACCAGCTGCCCTTTGACCCTGCCAACAACAACGAACCCC
TGCAGTTTGGTAGTGCCAGTGGCCCTCTGGTTCCAGAAGGCCTCATTGAGAATGGAGGGGAATCAAGCAA
GAAAAGAAAGAGAACAAATCCTCCTTCAGCTGATAATACTAGAACTCTGAATGTAGATTCTGCTGCAATG
ACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGGGAA
TTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAATAAC
TCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAGATC
ATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAAGGC
CACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGAAGT
GTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTTGCT
GAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTGGTTACCGAATTCGTCTGGGCTCTAGTACTGACA
AGAAGGACACAGGCCGGCTCCATGTTGACTTTGCCCAGGCTCGGGATGACTTATATGAGTGGGAGTGTAA
ACAGCGTATGCTAGCCAGAGAGGAGCGGCACCGTAGAAGGATGGAAGAAGAAAGAATGCGTCCACCATCC
CCACCTCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAAAAACTCAAAGATGATTCCA
AATTCTCGGAAGCTGTGCAGACCTTGCTCACCTGGATTGAGAGGGGGGAAGTGAACCGCCGCAGTGCCAA
CCACTTCTACTCCATGATCCAGTCAGCCAACAGCCATGTCCGCCGCCTGGTAAATGAGAAAGCTACCCAT
GAGAAAGAGATGGAAGAAGCAAAAGAGAAGTTCAAGCAGGCCCTTTCTGGAATTCTCATTCAATTTGAGC
AGATAGTAGCTGTGTACCATTCCGCTTCCAAACAGAAGGCATGGGACCACTTCACAAAAGCACAACGTAA
AAACATCAGTGTTTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGAATC
AGAAGAGAAGAAGAAATGGAAATGTCTGATGATGAAATAGAAGAGACAACAGAAACAAAAGAAACGGAGG
AATCAGCCTTAGTGTCACAGGCAGAAGCTCTGAAGGAGGAAAATGATAGCCTCCGCTGGCAGCTGGACGC
ATACCGGAATGAGGTAGAACTGCTCAAACAGGAACAAGGCAAAGCCCACAGGGAGGATGACCCAAACAAG
GAACAGCAGCTGAAACTTCTGCAGCAAGCCCTACAAGGGATGCAACAGCATCTGCTCAAAGTCCAAGAGG
AATACAAAAAGAAAGAAGCTGAGCTTGACCGAATCAAAGACGACAATTTACAGGTAGAACAACTGTTGGA
AAATTTTCATGAAAAGCAGGAAAACTGTGGTTCTAGACTGTGTGCATCAAGCCAAGAAGGTGAGCAACCT
CTTGAGAAGACCGCAGTCAGCAATCCTGTCAAATCTGAACGTGAGGCACTGCTAGTGGGGATCATCTCCA
CATTCCTTCACGTCCACCCATTTGGAGCCAGCATTGAATACATCTGTTCCTACTTGAATCGTCTCGATAA
TAAGGCAAGCTACCAAATACCCAGCAAATTAACCACTTCTCCACTCTTACCGATCAGCACCAGTGATGTG
GAATCTCTCATGAGTAGACTCCAGCATACCTTCAGACAGGAAATGACTGGAGTTGGAGCCAGCCTGGAGA
AGAGATGGAAATTCTGTGGTTTTGAGGGCTTGAAGCTGACCTGAAGCTCTTTGCCTAGAATTAGAGATCC
TGAAAATAAATGTGTGCTTGACAAGCTTAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5043650 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5043650 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17169 same embryo
 EMAGE:17170 same embryo
 EMAGE:17172 same embryo
 EMAGE:17173 same embryo
 EurExpress:euxassay_001555 same experiment
 MGI:4824557 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS