EMAGE mouse gene expression spatial database

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EMAGE:17184

Galntl4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 4 ( MGI:2446239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001578. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001578_01	euxassay_001578_02	euxassay_001578_03	euxassay_001578_04

euxassay_001578_05	euxassay_001578_06	euxassay_001578_07	euxassay_001578_08	euxassay_001578_09

euxassay_001578_10	euxassay_001578_11	euxassay_001578_12	euxassay_001578_13	euxassay_001578_14

euxassay_001578_15	euxassay_001578_16	euxassay_001578_17	euxassay_001578_18	euxassay_001578_19

euxassay_001578_20	euxassay_001578_21	euxassay_001578_22	euxassay_001578_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17184_wholemount_strong.wlz

17184_wholemount_moderate.wlz

17184_wholemount_weak.wlz

17184_wholemount_possible.wlz

17184_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17184_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

strong

regional

strong expression: see section 04 moderate expression: see section 01 02 03 05 18 19 20 21 22 23

foregut-midgut junction

strong

regional

strong expression: see section 13 moderate expression: see section 14 15 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

otic capsule

moderate

regional

moderate expression: see section 02 03 04 05 08 09 17 18 19 21 22 23

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

esophagus

strong

regional

strong expression: see section 12 13 14 15

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13

hindgut

strong

regional

strong expression: see section 13 moderate expression: see section 14 15

midgut

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 14 15 16 17 18

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 14 15 16

basisphenoid bone

strong

regional

strong expression: see section 02 23

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4778
Entity Detected:	Galntl4, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 4 ( MGI:2446239)
Sequence:	sense strand is shown >T4778 GTTCTCCTGAGCGGCGAGCGGAGGGCTGGTGGCATCTCCCAGGCAGCCAGCACTGGAGATGGAAAGCAAC GCGGCTGGGCCCTGGCAGTGACAAAGTTTCCCAGCCCTGCAGGTCTCGTCTCGCCACTGCCGGCCACCCG TGACCGCCTAGGGAAAAGCTCGGGGATTTTGAATTTGCGGGGGAAAAGCCCAAAACAAAAATCTAGACCC ACCTCGTTTTCTTTGTGGAGCTCTTTCTCAGGGTGGCCAGCGGCCGGAGCTGCAAAGTTGTGCGCCGCAG CTCACACTGCCAGGAGACCCGCCTTTGGAGAGACTTAGGTGCTGGCGCACCCAGAGACCCCAGACGTGCC AAGGGGGTTGAGCGCGGGGGAGTTTGGGGTGTACAGCGAGGAGGGGCCCCGGGTGCTCTATATGGAGGAA GGACCCCAGAATGGTGTGTACCAGGAAGACCAAAACTTTGGTGTCCACTTGCGTGATCCTAAGCGGCATG ACCAACATCATATGCCTGCTGTACGTGGGCTGGGTCACCAACTACATCGCCAGCGTGTATGTGCGGGGGC AGGAGCCAGTGCCCGACAAGAAGCTGGAGGAAGATAAAGGGGACACCCTGAAAATTATCGAGCGGCTGGA CCACTTGGAGAATGTCATCAAACAGCATATCCAAGAGGCTCCTGCCAAGCCTGAGGAGGCAGAGGCCGAG CCCTTCACGGACTCCTCCCTGTTTGCACACTGGGGGCAGGAGCTCAGCCCGGAAGGCCGGCGTGTGGCCC TGAAGCAGTTCCAGTACTACGGCTACAACGCCTACCTCAGTGACCGGCTACCCCTCGACCGGCCCCTGCC AGACCTCAGACCCAGTGGGTGCCGCAACCTCTCGTTCCCCGACAGCCTACCTGAGGTGAGCATCGTGTTC ATCTTCGTCAACGAGGCGCTCTCAGTGCTCCTGCGTTCCATCCACTCGGCCATGGAACGCACGCCATCAC ACCTGCTCAAGGAGATCATCCTGGTGGACGACAACAGCAGCAACGAGGAGCTCAAAGAAAAGCTGACTGA ATATGTGGACAAGGTGAACGGCCAGAAGCCAGGCTTCATCAAAGTTGTGCGGCACAGCAAGCAGGAAGGC CTCATCCGCTCCAGAGTCAGCGGCTGGAGGGCAGCCACAGCCCCTGTGGTGGCACTGTTCGATGCCCACG TGGAGTTCAATGTGGGCTGGGCGGAACCAGTCCTCACCCGCATCAAGGAAAACCGGAAACGGATCATTTC ACCATCTTTTGATAACATCAAATATGACAACTTTGAGATAGAAGAGTACCCGCTAGCCGCCCAAGGCTTT GACTGGGAGCTGTGGTGCCGCTACCTAAACCCACCCAAGGCCTGGTGGAAGCTAGAGAACTCCACAGCCC CAATCAGGAGCCCTGCCCTCATTGGCTGTTTCATTGTGGACCGGCAGTACTTCGAGGAGATTGGCCTGCT GGACGAAGGCATGGAGGTCTATGGAGGGGAGAATGTGGAGCTTGGGATCAGGGTGAGTGAGATTTCTCAC ACAGGGCTGAGCTCAGCTCCCATGATGGTATGGCAGTGTGGCGGGAGTGTGGAGGTCCTGCCCTGCTCTC GGATTGCCCACATCGAGCGAGCTCACAAGCCCTACACAGAGGACCTCACTGCCCACGTCCGCAGAAATGC CCTCAGGGTAGCTGAAGTCTGGATGGATGAATTCAAAAGTCATGTCTACATGGCATGGAACATACCACAA GAGGACTCAGGGATTGACATCGGGGACATCACCGCACGCAAGGCTCTGAGGAAGCAGCTGCAGTGCAAGA CCTTCAGGTGGTACCTGGTCAGTGTGTACCCAGAGATGAGGATGTACTCTGACATTATCGCCTATGGAGT GCTGCAGAATTCCCTGAAGACTGATTTGTGTCTTGATCAGGGCCCAGATACAGAGAATGTCCCCATCGTG TACATATGCCATGGGATGACACCCCAGAATGTGTACTACACAAGCAGCCAGCAGATCCACGTGGGTATCC TGAGCCCCACTGTGGATGACGATGACAACCGGTGTCTGGTGGACGTCAACAGCCGGCCACGTCTCATCGA GTGCAGTTACGCCAAGGCCAAGAGGATGAAGCTGCACTGGCAGTTCTCCCAGGGAGGCTCCATCCAGAAC CGCAAGTCCAAGCGCTGCCTGGAGCTGCAAGAGAACAGTGACATGGAGTTTGGTTTCCAGCTGGTGCTTC AGAAGTGCTCGGGACAACACTGGACTATCACCAATGTCCTGAGGAGCCTGGTGTCCTGACCCTCCACAGG CGCCACCGGCCACCGCTTTGCTACTGTGTAGCATCAGCTGCAATGCCGCCTGCTGTCACGTGGGGCTGCC CGGAGTCTGAGAGGGAGAGCCAAGTGGGTGGCCTCCCCCCACAAAAGAGCTTTGTATTTCCTATTCAATT TTCATGGAGTTTATAGAGAGACGCAGAGAGGTGGGGGAGGGAACAACATCCTGAACTATTTTACAACTGT AAATAGGGGACAAATGGAAAATATTTATAACTGGCAATAAAATATTATTGATTAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5044626 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5044626 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17185 same embryo
	EMAGE:17186 same embryo
	EMAGE:17187 same embryo
	EurExpress:euxassay_001578 same experiment
	MGI:4824988 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source