EMAGE mouse gene expression spatial database

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EMAGE:17186

Slc45a3 solute carrier family 45, member 3 ( MGI:1922082)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001583. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001583_01	euxassay_001583_02	euxassay_001583_03	euxassay_001583_04

euxassay_001583_05	euxassay_001583_06	euxassay_001583_07	euxassay_001583_08	euxassay_001583_09

euxassay_001583_10	euxassay_001583_11	euxassay_001583_12	euxassay_001583_13	euxassay_001583_14

euxassay_001583_15	euxassay_001583_16	euxassay_001583_17	euxassay_001583_18	euxassay_001583_19

euxassay_001583_20	euxassay_001583_21	euxassay_001583_22	euxassay_001583_23	euxassay_001583_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17186_wholemount_strong.wlz

17186_wholemount_moderate.wlz

17186_wholemount_weak.wlz

17186_wholemount_possible.wlz

17186_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17186_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 07 08 09 10 17 18

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

cerebral cortex marginal layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 17 moderate expression: see section 10 11 12 16

esophagus

weak

regional

weak expression: see section 12 13

renal cortex

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20 21

testis

moderate

regional

moderate expression: see section 07 08 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4784
Entity Detected:	Slc45a3, solute carrier family 45, member 3 ( MGI:1922082)
Sequence:	sense strand is shown >T4784 CCAGCCCCAGGGGTAGAAGAGGGGTCCTGGTAGAAGACTAGTGCCTGTCCTGGTATAGAGAGCACACACA GCGTGACCTTGGGTGGCAGCTGTTGTCAGCAATGAAGTCTGGTTGGGAGGCCCAGGTGCCTGGAAGCAAA ATGCTCGTGTGCGTTGCCTGTCGCTGGAGGAGACCAGGAGTCCTCAAGGGTACTTCAGCCGCAGCTTAGG CTGGCCAAGGATCTGACCGACGAGATGTGTCCCCATCAAGCAAGGCACTAGATGGTGACGTGTTTAGCGT GGGACGAGATGCTGAATTGGCACTAAAGGGCTGGCAGAAATGGGAACCTGGCTGCACCCTAGGAGGTTAG TGCTAGTGAGGAGGAGAAGCCACGGCAGGGCTGACTCAAAGCAGCTGTGGAGTATGTGAGTAGCCCCTGG AACCCTACCTGCCCTGTCCATCATGATCCAGAGGCTGTGGGCCAGCCGTCTGCTACGGCACCGGAAAGCT CAGCTCCTGCTGGTCAACCTGCTCACCTTTGGCCTGGAGGTGTGCCTGGCTGCCGGCATTACCTATGTGC CACCCCTTCTGCTGGAAGTCGGGGTGGAGGAGAAATTCATGACCATGGTGTTGGGCATTGGCCCAGTGCT AGGCCTGGTTTCTGTTCCACTCCTAGGCTCAGCCAGTGACCAGTGGCGTGGGCGCTATGGCCGCCGGAGA CCCTTTATCTGGGCTTTGTCCCTGGGTGTCCTGCTAAGCCTCTTTCTCATCCCGAGGGCTGGCTGGCTGG CAGGACTGCTGTACCCAGACACCAGGCCCCTGGAGTTGGCCCTGCTGATCTTGGGAGTGGGGCTGCTGGA CTTTTGTGGCCAGGTGTGCTTTACTCCATTGGAGGCCTTACTCTCCGACCTCTTCCGGGACCCAGACCAC TGCCGCCAAGCCTTCTCTGTCTACGCCTTCATGATCAGCCTTGGGGGCTGCCTGGGCTACCTCTTACCTG CCATTGACTGGGACACCAGCGTTCTGGCCCCCTACCTGGGTACTCAGGAAGAATGCCTCTTTGGCCTCCT CACCCTCATTTTCCTCATCTGCATGGCAGCCACTCTGTTTGTGACGGAGGAGGCAGTACTGGGCCCACCC GAGCCGGCAGAAGGGTTGTTGGTCTCTGCCGTGTCGCGCCGATGCTGCCCATGCCACGTTGGCCTGGCTT TCCGGAATCTGGGTACCCTGTTTCCCCGGCTGCAGCAGCTGTGCTGCCGCATGCCTCGCACCCTACGCCG ACTCTTTGTGGCTGAGCTGTGCAGCTGGATGGCACTTATGACTTTCACACTGTTCTACACGGACTTCGTG GGAGAGGGGCTGTACCAGGGTGTACCCAGAGCCGAGCCAGGCACCGAGGCCCGGAGACACTATGATGAAG GCATTCGAATGGGCAGCCTGGGGCTCTTCCTGCAGTGTGCCATCTCCCTGGTCTTCTCCCTGGTCATGGA CAGGCTGGTACAGAAGTTCGGCACACGGTCAGTCTATCTGGCCAGTGTGATGACCTTTCCTGTGGCTGCC GCTGCCACCTGCCTGTCCCACAGCGTGGTGGTAGTGACAGCCTCAGCTGCCCTCACCGGGTTCACCTTCT CGGCCTTGCAGATCCTGCCTTACACGCTCGCCTCCCTCTACCACCGTGAGAAGCAGGTGTTCCTGCCCAA ATACCGAGGGGACGCTGGAGGTAGCAGCGGTGAGGACAGCCAGACAACCAGCTTCTTGCCAGGCCCTAAG CCAGGAGCTCTCTTCCCCAATGGACACGTGGGCTCTGGCAGCAGCGGCATCCTGGCCCCTCCACCTGCAC TCTGTGGGGCCTCTGCCTGCGATGTCTCCATGCGAGTGGTGGTGGGTGAGCCACCTGAGGCCAGGGTTGT TACGGGACGGGGCATTTGCCTGGACCTGGCCATTCTGGACAGTGCCTTTCTGCTGTCCCAGGTGGCTCCG TCCCTGTTCATGGGCTCCATTGTCCAGCTGAGCCACTCTGTCACTGCCTATATGGTATCAGCTGCAGGCT TGGGTCTGGTCGCCATTTACTTTGCTACACAGGTAGTGTTTGACAAGAACGACTTGGCCAAATACTCAGT GTAGAATTGTGTAAGGCATCAAAGAGAGGGTCTGCCTCATGGGTTCTCAGCCCTTAGAGGGCTGCAGAGC TGGCCTCTCCAGGTCTTTGTCGCCTAAGTGGCTCTCTGCTGCCACCCTAAGGCAGTGAGGTGTATTGTTG CACAGATAGGAGCCAGAGCTTTCGGGGCTCTGGCTTCAGAGTCTGGCTGGCCTACTGGCAGCCTCTCGCA TGGAAGTCATTCTGAAGTTCTGCAGGGATTGAAATGTGGTCTGTTCAGGTGTCCTGTATCAAGGCTCCAG GTTAACAGCTAACCTCCTGCTTAAGTCACCAGAGGGAGGATTTGGGGGACCTGAACAAACTTCCCTCCAA GTCCCTTGACCAGATTCTGCCACTGTTGCTCTTGCATGGGAGTTTCTTGTAGGATGCTCTCCTCCACAGG GTTTGGCAGGGTGGAAGTTGGAAGGAGGAACCCTAGCAGGCAAGATGAAACCCCGTGATCTACAGCACTG CCTTTCTGCTGAGTCTCCCACCCCCCACTTGTTTCAGGAAATGGCTCAGCTTGTTGGCCACAGAGACACA GGCCTTTAAATATTTAATTTATTATTTATTTAACAAATGAGAAGAAAATTCATTCCCAGCCTTTTTATGT TGTGCCTAGGAGTTGAGTAGGGTGGGATTCCCCAAAACTGGGTCCCCTAAAATAGCTGGTCACTGTGTTA GATATTGCCAGACTCCTCTTCTGGGGTGATAGATATGGTCCCCCAAACTGCTCAGCCCAGTACCTCGGGC AATCTCTTCATCCTAATTGAGAGCCATAAGATGTTCCTGCTCAAGGATTGGGACCCAAGGTCTCCACAGC ATACCAAATCCCAATTCTCTCTTGACCCAGTTTGGTTCCCTGATTGCCCACTTCTCCCTATGGGAAAGGA CTGTGAAGAGATTGCCCTTCCAAGTCTGTCCCCTGGCTCCAGGACCTCTCTATGGGGCTTTCTGAATCCT AAGCACAAATGTGGTTCCTTAGGCTTCTGCCCATCTCAGCCTCTGGGGCATATCTGTCACAGCCTGGGGA ATCTCACACAAACTCAGGAGTACTCCCCGCCAGAGCCAGTGAGCTCATCACGTCGAGGGGGATTTCAGTG CTGATTGCAATAATGTTATCTATGTCTTATTTATTTAGCAGAGTAAATATTTTATACCGTATGTGATAAA TCAGAGTATAATGTTTATGGTGATGAAATTAAAAGCTTCGTATATGTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5050610 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5050610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17185 same embryo
	EMAGE:17184 same embryo
	EMAGE:17187 same embryo
	EurExpress:euxassay_001583 same experiment
	MGI:4828237 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source