EMAGE mouse gene expression spatial database

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EMAGE:17196

5430407P10Rik RIKEN cDNA 5430407P10 gene ( MGI:2442238)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001603. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001603_01	euxassay_001603_02	euxassay_001603_03	euxassay_001603_04

euxassay_001603_05	euxassay_001603_06	euxassay_001603_07	euxassay_001603_08	euxassay_001603_09

euxassay_001603_10	euxassay_001603_11	euxassay_001603_12	euxassay_001603_13	euxassay_001603_14

euxassay_001603_15	euxassay_001603_16	euxassay_001603_17	euxassay_001603_18	euxassay_001603_19

euxassay_001603_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17196_wholemount_strong.wlz

17196_wholemount_moderate.wlz

17196_wholemount_weak.wlz

17196_wholemount_possible.wlz

17196_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17196_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 01 02 03 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 06 07 12 13 14

foregut-midgut junction

weak

regional

weak expression: see section 10 11

vibrissa

weak

regional

weak expression: see section 04 05 16 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 14

trigeminal v ganglion

weak

regional

weak expression: see section 07 12 13 14 15 16 17

vagus x ganglion

weak

regional

weak expression: see section 06 12 13

otic capsule

moderate

regional

moderate expression: see section 01 02 03 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14

stomach

weak

regional

weak expression: see section 06 07 08 09

midgut

weak

regional

weak expression: see section 09 10 11

basisphenoid bone

moderate

regional

moderate expression: see section 15 16

exoccipital bone

moderate

regional

moderate expression: see section 02 03 04 15 16 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 06 15 16 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4795
Entity Detected:	5430407P10Rik, RIKEN cDNA 5430407P10 gene ( MGI:2442238)
Sequence:	sense strand is shown >T4795 CGATCGGCGTGCGTGCGTGCACGACTTGCTGGCCGGAGGACGGCGTGAGCCCCGAGGGTCTCAGTCGGCG TGGACACCTGCGACCCGCGGAGGGGACTTGGCTCGGTGAGTTCCGCCCTACTGAGCGAGGCCAGGCCAAG CGCGGGACACCGGGAGCGGCAGCAGCTGTCGGGATCATCCAGGGCTCCCGGTCCGGGTCTTGGTAACTGG GGAAGCCCTTTGCGCGCAGCTGGGGCTCTGTCGTCGCTGAGAAGACCTCCTTCCCTGCCTCAGAATGGAA CCCTGGCTCCTACCCTGTTTTCTGTGAGCTAGCTAGTGACAGCTTTGTGTGGGGATGCCTGGGAACCAGC AGAAACCAGAGTCTCTGGAGACGGACTCTGACACGTCGCCCAGCTGCGGGCTCAGCGACTTGAGCAGAGG TGGCAGTCTGGAGAGCCGGTGCAGCAGTTCGCGGTCCAGAAGTTTCACTATGGATGACGAGAGCCTGAAG CACCTCACACATGAGGAAAAGGACGTTATCCTGTTCTTTGAAGAGACTCTTGATTCCCTGGAATATGACT TTGACGAGCCAGCCCTGTGTGACAGTGGCATTCACTGCCACTCTCCACAGTCTCTGGAAGAGAGCCCTTC CAGCCACTCTGAGCCCGAAGATGTCATCGATTTAGTGCAGCCCGCACCTGCGTCTGGGGAAGCTGAGAGC CTTCCAGATATGCCTCAAGTAACAGGGGCCCCATCTGATACAAAACATGGCACTCCATTTCTTGAAGGTG GGAAACAAGCCGCTGAGAATTCCCTGCCACCACCAGACTCCAGGGGCCCTGAGGTGTTTCCTTTGCCGCC CTCCCTGCCTGTCCCCGCTCCCTCTGCCCCGCGAAAGGAGCTGATGTCCCCCTCTCCTCCAGCAGAGCAT CCCAAACTGCTGCGTTCTGTGCCCACTCCTCTGGTGATTGCTCAGAAAATATCTGAGAAGTTGGCGGGCA ATGAAGCCCTGTCTCCCATATCCCCTTCAAAGGAAGGCAGGCCTGGGGAATGGAGGACACCGACTTCTCC AGCTTCTAGAAACGGAGACCATGTCGGCGTGTGGCATCGACACACGACCCAGTCAGCACCCAAGGTCCAT CGCTTCCCCAGCAACATAAGCGTGACCAACAGCGCAGGCAAGGACTTCAACAAGACCATCTCCAAGGCGG CTGTCAATGTGCAGGAGCGCAAGGCCCAGGTCCTGGCCAACATCAACGGCATGTCCTTCATCGCTGCGGG CGACACATCATCAGAAGAGCGGTGGCAGAAGGCGGAAGAGCAGAGGAGTGGCTCTGCGGATGGAGCACGC ACCCTGGGCAGAGCGGGCATGGCCGGCGAGCCCGGGGCACCTTGTGCAGGAGTCCCGGCCAGGGCGCAGC AGTCCCGGGCTGTGCAGACAGAGCAGCCACCGGCGCTGGCGAACGGCTTCCAGAGCGTACACGAGGCCCT GCGCAGCGAGCCCAGCTCCTTCGTCCCCACCAGCAAGACCATCACCTTCCGGCCTGACCCCGCTGTGACC GGCAAGCTGGCGCGCCAGAATGCCAGCCGCAGCCTATACGAACCGCGCCCGGACGGCAGCCAAGATGCCC GCAAGCGCACGGGCTCGCTCCCCCGGGCGGTGGGTTTCCGGCCGCAGGGCATCACAGTGCAGTTCTCTGG CCGCGGCTCCACTGAGGAAGCTCGGCGCGAGGCTCTGCGCAAACTCGGGCTACTGAAGGAGAACTTGTGA GGGCCATGGGTGAGACTGCTGGAATGGCCTGGGTGGAACTTGTATGTCCACACTGGGGGGTCCAGTTCTG GAGCTGGGTGGCACGAAGTGCTGCTTGTCGGGAGAGAAGCTTCTGCCCCAATCCCAGTGCAGCGCGGAGT CAAGAGACCTGCAGGGCAAGGACAGCGCCCCCCCCCCCCGTGCCCCCCAGCTGTCCTTTTCCTTTTCCGG GGTCTTGGAATTAGGACCTCTGGGCCTCCATGACTGCGACTTGGTCCAGACTCTGATACTCAGGATTCCC TCTCCTCTTCGAAAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5052194 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5052194 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17197 same embryo
	EMAGE:17198 same embryo
	EurExpress:euxassay_001603 same experiment
	MGI:4822761 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source