EMAGE mouse gene expression spatial database

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EMAGE:17214

Edn1 endothelin 1 ( MGI:95283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008867. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008867_01	euxassay_008867_02	euxassay_008867_03	euxassay_008867_04

euxassay_008867_05	euxassay_008867_06	euxassay_008867_07	euxassay_008867_08	euxassay_008867_09

euxassay_008867_10	euxassay_008867_11	euxassay_008867_12

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17214_wholemount_strong.wlz

17214_wholemount_moderate.wlz

17214_wholemount_weak.wlz

17214_wholemount_possible.wlz

17214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

vibrissa

strong

regional

strong expression: see section 01 02

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

pons mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

midbrain mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12

trigeminal v ganglion

strong

single cell

strong expression: see section 01 02 03 04 10 11 12

trigeminal v nerve

strong

single cell

strong expression: see section 09

spinal cord mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09

cochlea

strong

regional

strong expression: see section 04 05 07 08 09

utricle

strong

regional

strong expression: see section 01 02 03 04 09 10 11 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05

rectum

strong

regional

strong expression: see section 06

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4817
Entity Detected:	Edn1, endothelin 1 ( MGI:95283)
Sequence:	sense strand is shown >T4817 GCCGGGTCTTATCTCTGGCTGCACGTTGCCTGTGGGTGACTAATCACACAATAACATTGTTTAGGGCTGG AATAAAGTCAGAGCTGTTTACCCCCACTCTATAGGGGTTCAATATAAAAAGGCGGCGGAGAACTGTCCGA GTCAGAAGCGTTCCTGCACCGGCGCTGAGAGCCTGACCCGGTCTGCTCCGCTGTCCTTGCGCGCTGCCTC CCGGCTGCCCGCGACGCTTTCGCCCCAGTGGAAGGGCCACTTGCTGAGGACCGCGCTGAGATCTAAAAAA AAAACAAAAAACAAAAAACAAAAAAACCCAGAGGCGATCAGAGCGACCAGACACCGTCCTCTTCGTTTTG CATTGAGTTCCATTTGCAACCGAGTTTTCTTTTTTTCCTTTTTCCCCACTCTTCTGACCCCTTTGCAGAA TGGATTATTTTCCCGTGATCTTCTCTCTGCTGTTCGTGACTTTCCAAGGAGCTCCAGAAACAGCTGTCTT GGGAGCCGAACTCAGCACCGGAGCTGAGAATGGAGTGCAGAGCCCCCCTCCCAGCACACCCTGGAGACCC CGCAGGTCCAAGCGCTGTTCCTGTTCTTCCTTGATGGACAAGGAGTGTGTCTACTTCTGCCACCTGGACA TCATCTGGGTCAACACTCCCGAGCGCGTCGTACCGTATGGACTGGGAGGTTCTTCCAGGTCCAAGCGTTC CTTGAAAGACTTACTTCCCAATAAGGCCACAGACCAGGCAGTTAGATGTCAGTGCGCTCACCAAAAAGAC AAGAAGTGCTGGAATTTCTGCCAAGCAGGAAAAGAACTCAGGGCCCAAAGTACCATGCAGAAAAGCTTAA AAGACTCCAAGAAAGGAAAACCCTGTTCCAAGTTGGGAAAGAAGTGTATCTATCAGCAGCTGGTGGAAGG AAGGAAACTACGAAGGTTGGAGGCCATCAGCAATAGCATCAAGGCATCTTTTCGTGTTGCAAAGTTGAAA GCTGAGCTCTATAGAGACCAGAAGTTGACGCACAACCGAGCACATTGACTACAGAGCTCCCCCAGTGTTT TGGAAGTCATCCCTTATATAGTGCGAGCCCCATGGCCAGCTCTGCACTCTCCATGCTGGCTGGGATCTTA GCAAGACCATCTGTGTGGCTTCTACAGTTTCTTGTTCAGACGGGCAGGACCAGCATCCTTGATCCAAACA TTCCAAGAAAGGCTGAGGTGTTCCCTAGCCTGTCTGCGTCCGCTGGGAGCGAGTGCCTTTCTGCCTCTTC TTGCCGGTTGGGAATGACAGAGGACTTCTCAGAGAGCAGAGACACGATGCCATTCTAGAGTGGCATCACT CAGAGAGCCAGGAGATTCCACAGCAGGGCGGGGTTTCTGTAGAAAGTCCTTAGGGAGTGTTCGTGTCTGA CTCAGGCGCCTGGCACATTTCAGGGAGAAACTCCAAAGTCCATGCAAAAATTTTTCTGAGGAATGCACAA ATTGAAAACATACTCGAAGGACAAACACTTGAGTTTAAAAAAAAAAAAAAGACAATTTTTAAGTTGTAAA ATGCAAAACTCAAAGCAACTGTTACTACTGTACATTGGGATGTATTTCATGAATATGAGTCTACCTCACC TTTCTGCACTGTCATACACATTCTCACCTTTAATTATTGCCTCCCTGTATTCTCTCCTCTCCGCTATCCG GGTTCCCAGTCCCCTATACTGAATCCTTGCCTCACAGAAATTGAGTCAGAAGTAGCTGAAACATTGCCAT GCTAATCTGCTAGCTCTAATCTATGAGAAAAAAGGATGACAGAATCTGGCCCCTGCCCTTGACCTTGGGA AACACAATGGTTTAGAGTTGTTTGTGTACATGTTGAAAACCTGGTCTGTGCTTTTTAGCAGTAAAAATAT GTTCCCGTATGTATTGTAACTGGCTAATGGAAGAGGTTAGATTGAATCTTGATGTACTTATTTTTTTATA GATATTTATATTCAAACAATTTATTCCTTATATTTACCATGTTAAATATATGTCTGGGCAGGCCATATTG GTCTATGTATTTTTTAAAAATATATATTTCTGAATGAAATTGAGAACATGCTTTGTTTTGCCTGTCAAGG TAATGATTTTAGAAAATAAATATTTTTCCCTACTGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5059522 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5059522 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17212 same embryo
	EMAGE:17210 same embryo
	EMAGE:17213 same embryo
	EMAGE:17211 same embryo
	EurExpress:euxassay_008867 same experiment
	MGI:4824473 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source