Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17218

5730469M10Rik RIKEN cDNA 5730469M10 gene ( MGI:1917814)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218
"Pseudo-wholemount" of euxassay_008864. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008864_01 euxassay_008864_02 euxassay_008864_03 euxassay_008864_04
EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218
euxassay_008864_05 euxassay_008864_06 euxassay_008864_07 euxassay_008864_08 euxassay_008864_09
EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218
euxassay_008864_10 euxassay_008864_11 euxassay_008864_12 euxassay_008864_13 euxassay_008864_14
EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218
euxassay_008864_15 euxassay_008864_16 euxassay_008864_17 euxassay_008864_18 euxassay_008864_19
EMAGE:17218 EMAGE:17218 EMAGE:17218 EMAGE:17218
euxassay_008864_20 euxassay_008864_21 euxassay_008864_22 euxassay_008864_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17218Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17218_wholemount_strong.wlz
17218_wholemount_moderate.wlz
17218_wholemount_weak.wlz
17218_wholemount_possible.wlz
17218_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17218_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
sublingual gland primordium
strong strong
regionalstrong expression: see section 09
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 08 16 17 18 19
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 02 03 04 05 06 16 17 18 19 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 19 20
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 17 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 16 17 18
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 08
spinal cord
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 16
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 09 16 17
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12 13 14
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 18
cochlea
strong strong
regionalstrong expression: see section 05 06 07 08 09 16 17 18
utricle
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 17 18 19 20 21
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 20 21 22 23
cornea epithelium
strong strong
regionalstrong expression: see section 01 02 03 20 21 22 23
retina
moderate moderate
regionalmoderate expression: see section 01 02 03 20 21 22 23
naris
strong strong
regionalstrong expression: see section 10 11 13 14 15
nasal cavity
strong strong
regionalstrong expression: see section 13
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18
naso-lacrimal duct
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 16 17 18 19 20
stomach
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10
rectum
moderate moderate
regionalmoderate expression: see section 13 14
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
lower jaw incisor
strong strong
regionalstrong expression: see section 09 10 11 14 15
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 17 18
upper jaw incisor
strong strong
regionalstrong expression: see section 09 10 11 14 15
upper jaw molar
strong strong
regionalstrong expression: see section 06 18
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
bladder
strong strong
regionalstrong expression: see section 13 14
kidney calyx
strong strong
regionalstrong expression: see section 07 08 09 10 11 16 18 19
kidney pelvis
strong strong
regionalstrong expression: see section 09 17
ureter
strong strong
regionalstrong expression: see section 10 11 15 16
renal cortex
strong strong
regionalstrong expression: see section 07 08 09 10 16 17 18 19
lung
strong strong
regionalstrong expression: see section 09
left lung
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 10 11
right lung
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4820
Entity Detected:5730469M10Rik, RIKEN cDNA 5730469M10 gene ( MGI:1917814)
Sequence:sense strand is shown

>T4820
GGCACCTGCGTCCGCTGGCGCAGCTCAGGGGCCAGTGAGGTCCACGGAGGCGGGGTCCTTGGGGCGCAGG
ACCCAAGTTTCTTCTCCATGGGTATGTGGTCCATCGGTGTGGGAGCTGTGGGGGCCGCTGCTGTGGCACT
GCTCCTGGCCAACACAGACATGTTTCTGTCCAAGCCCCGAAAAGCAGCACTGGAATATTTGGAAGACATA
GACCTGAAAACACTGGAGAAAGAACCAAGGACATTCAAAGCAAAGGAGCTGTGGGAGAAGAACGGGGCTG
TGATTATGGCTGTACGCAGGCCAGGCTGCTTTCTCTGCCGAGCGGAAGCAGCAGACCTGATGTCCTTGAA
GCCCAAGTTGGATGAGCTAGGTGTCCCTCTCTATGCGGTGGTGAAGGAGCAGGTGAAGAGAGAAGTGGAA
GATTTCCAACCTTACTTCAAAGGGGAAATCTTCCTGGATGAAAAGAAAAAATTCTATGGTCCAGAGAGGC
GGAAGATGATGTTCATGGGCCTCATCCGATTGGGGGTGTGGTATAACTCCTTTCGAGCCTGGAATGGAGG
CTTCTCTGGAAACCTAGAAGGCGAAGGCTTCATCCTTGGAGGAGTTTTTGTGATAGGATCTGGAAAACAG
GGCATCCTTCTTGAGCACCGAGAAAAAGAATTTGGAGACAGAGTGAACCCACTCTCTGTTCTGGAGGCTG
TAAAGAAAATCAAGCTACAGACTCCAGCGTCCGGGAGAAGCTGATCCTGTACAGCTGCTGGCTGGGAGAG
AACGCACGGGGCCTGTGCTGTGTTCATCAGATGAGCTGTGCTTTCCCCAGGGCCCCAAGAGCCAGAAGGC
CCTTGCACCATATTTACTAAGAATTGGTGATGCATTTTAACATTGTCTGTTTAGGTCCCAGAGCCTTTAA
CATTCCGTTTAGGCCCAGTAGGGCAAATAGGGTCCCAGACAGAACAGAGTTCAATCTAACAAACCAGTGA
GAGTTATTTGAGGAAAGATCTAGAAAATTTAATGTTTAAAGTTTACTGCTGAAGCTTCTTTCAAAGGAAT
ATGTCCTAAGTGCCGGTATTTGAAGTGAAGTTTATTTTCATGCCTAGTTAAAAAACAAACAAACAAACAA
ACAAACCTGAAATCTGAGAAAAGTGCGGCTAAGAAACAGCTCAGTGGTGGAGCATTTACCTAACATGCAC
ATGGCCCTAAGTGGGATACCCAGACAGACAGACAGACAGACACACACACACACACACACACACACACACA
CACACTCTCTCTCTCTCTCCTAAGAAAAGCTGATGGCTGACTTGATAATTAGAGAATTATCCTTTTGTTT
GATGTGTTTTCTGTTAACAACTTGTTACTTAAGGTCAGAGTACCACATAAAGCAATTGCTGTGCTGTGTT
CTTGGATAAATTACACAGGTAAATTACAAAGTGAAGGTTCTGTGGTCTTCCCTTATAGACGCAGAGATTT
GCTTCCTTGGGAAAAACTGTCACCTGGGAAACTGTCACCTCTAATAAAAATATTCACCAGGAAAAAAAAA
AAAAAA
Notes:The probe template was PCR amplified from IMAGE:5064081 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064081 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17216 same embryo
 EMAGE:17215 same embryo
 EMAGE:17217 same embryo
 EurExpress:euxassay_008864 same experiment
 MGI:4822767 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS