EMAGE mouse gene expression spatial database

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EMAGE:17257

4930506M07Rik RIKEN cDNA 4930506M07 gene ( MGI:1918903)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001630_01	euxassay_001630_02	euxassay_001630_03	euxassay_001630_04

euxassay_001630_05	euxassay_001630_06	euxassay_001630_07	euxassay_001630_08	euxassay_001630_09

euxassay_001630_10	euxassay_001630_11	euxassay_001630_12	euxassay_001630_13	euxassay_001630_14

euxassay_001630_15	euxassay_001630_16	euxassay_001630_17	euxassay_001630_18	euxassay_001630_19

euxassay_001630_20	euxassay_001630_21	euxassay_001630_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17257_wholemount_strong.wlz

17257_wholemount_moderate.wlz

17257_wholemount_weak.wlz

17257_wholemount_possible.wlz

17257_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17257_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 16 17 18

foregut-midgut junction

strong

regional

strong expression: see section 10 11 12

diencephalon

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 19 20 21

telencephalon mantle layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 13 15 16 17 18 19 20 21 22

telencephalon marginal layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

olfactory cortex mantle layer

strong

homogeneous

strong expression: see section 08 15 16

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 14 15 16

hindbrain

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 06 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 16 17

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

cervical ganglion

moderate

regional

moderate expression: see section 07 14

thoracic ganglion

moderate

regional

moderate expression: see section 10

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 13 14 15 16

cornea stroma

moderate

regional

moderate expression: see section 01 02 03 21 22

neural retina

strong

regional

strong expression: see section 01 02 03 21 22

naris

moderate

regional

moderate expression: see section 11 12 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 10

stomach

moderate

regional

moderate expression: see section 01 02 03 04 06 07

hindgut

strong

regional

strong expression: see section 10 11 12

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

bladder

strong

regional

strong expression: see section 10 11

urethra of male

strong

regional

strong expression: see section 11

lung

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 10 11 14 15 16 17 18 19 20 moderate expression: see section 12 13

trachea

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5672
Entity Detected:	4930506M07Rik, RIKEN cDNA 4930506M07 gene ( MGI:1918903)
Sequence:	sense strand is shown >T5672 CCACGCGTCCGCGGACGCGTGGGTCGCAGCCGCACGCCGCGCCACCGGCATCTTGCTTTCTGCTCCCCCT CCCCCGTGTCCCCCTCGCCTCTCCCTCCTTGCCTTTTATCCCCAGCCCCACCCGCCAAAATGAACAGCTC GGACGAGGAGAAGCAGCTGCAGCTCATCACCAGCTTGAAGGAGCAAGCCATTGGCGAATATGAAGACCTT CGAGCAGAGAACCAGAAAACAAAGGAGAAGTGTGACAAAATTAGGCAAGAACGAGATGAAGCTGTTAAAA AACTGGAAGAGTTTCAGAAAATTTCACATATGGTTATAGAGGAGGTGAATTTCATGCAGAACCATCTTGA AATAGAGAAGACATGTCGAGAGAGCGCTGAAGCCTTGGCGACAAAGCTAAATAAAGAAAATAAAACACTG AAAAGAATCAGCATGCTATACATGGCCAAACTGGGGCCAGACGTAATTACAGAGGAGATAAACATTGATG ATGATGACCCAGCCACCGACACAGATGCTGCGGCTGAGACCTGTGTGTCTGTTCAGTGTCAGAAGCAAAT CAAAGAACTTCGAGATCAAATTGTGTCTGTTCAGGAAGAAAAGAAGGTGTTAGCCATCGAGCTGGAAAAC CTCAAGAGCAAACTCGGGGAGGTGATGGAGGAGGTAAATAAAGTTAAGCAAGAAAAAGCTGTTTTAAATT CAGAAGTCCTTGAGCAGAGGAAAGTCTTAGAAAAATGCAACAGAGTGTCCATGTTGGCTGTTGAAGAGTA TGAGGAACTGCAAGTGAACCTGGAACTGGAGAAGGACCTTCGCAAGAAAGCAGAGTCTTTTGCACAAGAG ATGTTCATTGAACAAAACAAACTGAAGAGACAAAGCCACCTTCTGCTGCAGAGCTCCCTTCCTGACCAGC AGCTTTTGAAAGCTTTAGACGAAAACGCAAAACTTATCCAGCAGCTTGAAGAAGAGAGGATCCAGCATCA GAAAAAGGTCAAAGAGCTGGAGGAGCGGCTGGAGAATGAAGCACTTCACAAAGAGATCCATAACCTCAGA CAACAGCTGGAGCTTCTGGAAGACGACAAGAGGGAGCTAGAGCAGAAATACCAGAGCTCGGAGGAGAAGG CCCGGAACCTGAAGCATTCAGTGGATGAACTTCAGAAGCGAGTGAACCAGTCTGAGAATTCGGTACCTCC CCCGCCTCCTCCTCCTCCACCTCTCCCCCCTCCACCTCCCAATCCAATCCGGTCCCTCATGTCTATGATC CGGAAGCGATCTCACCCCAGTGGCAATAGTGCTAAGAAAGAAAAGACAACTCAGCCAGAGACAGCTGAGG AAGTCACAGACCTGAAGAGGCAAGCAGTGGAAGAGATGATGGACAGAATTAAGAAGGGAGTTCATCTTAG ACCGGTTAACCAGACAGCCAGACCCAAGGCAAAGCCAGACTCTCTCAAGGGCTCAGAAAGTGCGGTGGAT GAGCTGAAGGGAATCCTGGGGACACTTAACAAATCCACTAGTTCGAGAAGCTTAAAATCCCTTGGCCCTG AGAACAGTGAAACTGAGTTAGAGAGGATTTTACGTCGCAGAAAGCTGACGGCAGAAGCAGATAGCAGTAG TCCTACCGGGATATTAGCCACCTCAGAGTCCAAGTCCATGCCAGTGTTGGGTTCTGTATCCAGTGTAACA AAATCAGCCTTGAACAAGAAAACTCTGGAGGCAGAATTCAACAACCCGTGTCCCCTAACACCTGAGCCAG GTGAAGGGCCCCGTAAATTGGAAGGATGCACGAATCCCAAGGTTACCTTTCAGCCTCCCAGTAAAGGTGG ATACAGGAGAAAATGTGTGGGCAGTGAAAACCAAGCTGAGCCAGTTGTAGTATTAGATCCTGTTTCCACA CACGAACCCCAAACCAAAGACCAGGCTGCTGAAAAAGACCCAACTCAATTCGAGGAGGAGGGAGGTGAAA CCCAACCAGAATACAAAGAAGACAGCGGTGGGAAAACAGGAGAGACGGACAGTTCCAACTGCTGATCTGA AACCAGAGGCTGGCAGGTTGGTGGGGCCTTCTCAAGGAGCACGTGGCTCGTCTGCGTGTAACCGGCAGGA ACTGTTGGACATTCCTTTGTTCTGGCCACACACTTCTTTGCTGGTATCACTTTGTAAGTAGCAATCATAA GTAAGCTGTTTAGCAAAATGCATCTTCTGGGTAGTTTTTAATCTTTACAGGAGTGAGGGTTCTTTCCGGT TTCTATATGAAGTATGACTTCCCTGAGGCGGTGGCGGAACTCAGAGGTTGGTGTCTTTACAGGGAACATA TGTACCCCTCATCAAATTATTCACGGGAAAATTCAAGGTGGTTGCTGGTAACTGTATAAACAGAGACCAC CAAACTTAACAATTATCTTAAAGGCACCCTTTAATCCAAGCCCTAGGGCATGGAGAGACCAAGGCAAGAG GATCACGTGAGCCTGAGTCTTAGTTTGGATCACACAGTACAACTCCATATAAAGAACATATAAAGAAAAG AAGGCCCGCAAGAGTCTCTGGGGTCTGGTCTTCTGATGGGAAACTGCAGCAGCCTCAACGTCAGTGGGTC CTATAGCGTCTCTGTTTTGAAGGGTGCGTGAGACCTTCCGTCTCTGAGCTCAGGAGGTGGCATCATGGAC TCTTAGGTCTCAATTGAACACAGTTGACTATTGTGTGGAAATCATGACCCTGTGTCCAAGGCTTTACAGA GTCCTTCAGGGTGATGTCTTGGCAGCATCAACAGAGAATTGGCTTAGAGGGACGCTCAAACCTTGAATTT AACAGGGATTCCTCGGTAAGCTACTAGTTCTGTTCCATGAGTTAACTGTGTTTTCCCACACATTCACGTG CCACAAGTACCATATTTTACACCAGCTCTCACGCACAGTGAAGATAAGTGACAAGCACACATTGTTTTTC TCCCTTCACTGCGGATCTATACTACATGGGTCTTTTCTTGTTTTGTTTTGTTTTGTTTTGTTTTTTAGAG GAAATTAAGTGGTAGTTTCTAAGAATACAATGTTTCACACTACCACGGTGAATAAATAGAAATGTAATCA GGGATTTAAAAAAAAAAACTTATGCAGGTTTTCAAAGTTGATTGTTTCAAAATTGGTGTTTATTTAAAAT AAGTGGTAATGTACTTGAGTGCACTTTTTATGATAATGATTCAGTAATGGTAATTTTACTATTAAGGAAA TGGAAAGGCTTAGTTTTGTTAGCATGACTCGGCATGTAGCTGTCAGGTGCTCTGCACCTGAGGGCAAAAG AAAATGATAACAATTGCAGTAGTTGTATCCTATTGTATTTTTGCACGTGTGCTACTAGCGTGGGCCTGGG GAGGTGGGAAGGCAGGTGAGGACACTTCCTCGGCTTGGGGACAATTCTCTTTCTGTAGGGTCAATGGGCT TGACTGTTTTCCCTGTCTGTCAGTGCTGATTTTATATCCCTGTCAGTTTACTCATGGGGAATTAAAACGC GATTTTTTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2599101 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2599101 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17256 same embryo
	EurExpress:euxassay_001630 same experiment
	MGI:4822732 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source