EMAGE mouse gene expression spatial database

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EMAGE:17266

Psat1 phosphoserine aminotransferase 1 ( MGI:2183441)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001596_01	euxassay_001596_02	euxassay_001596_03	euxassay_001596_04

euxassay_001596_05	euxassay_001596_06	euxassay_001596_07	euxassay_001596_08	euxassay_001596_09

euxassay_001596_10	euxassay_001596_11	euxassay_001596_12	euxassay_001596_13	euxassay_001596_14

euxassay_001596_15	euxassay_001596_16	euxassay_001596_17	euxassay_001596_18	euxassay_001596_19

euxassay_001596_20	euxassay_001596_21	euxassay_001596_22	euxassay_001596_23	euxassay_001596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17266_wholemount_strong.wlz

17266_wholemount_moderate.wlz

17266_wholemount_weak.wlz

17266_wholemount_possible.wlz

17266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 moderate expression: see section 09 10 11 12

submandibular gland primordium

strong

regional

strong expression: see section 05 06 16 17

vibrissa

strong

regional

strong expression: see section 02 03 04 05 18 19 20

hypothalamus ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 14 15

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 19 20 21

pons ventricular layer

strong

regional

strong expression: see section 07 08 11 12 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12

cranium

strong

regional

strong expression: see section 01 02 03 04 05 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5681
Entity Detected:	Psat1, phosphoserine aminotransferase 1 ( MGI:2183441)
Sequence:	sense strand is shown >T5681 CCGACTCAGCAGGCTGAAGCCCGCTCTGCCTTTGCATCCGGAGACGCGGGCTGTTCGCGTGGTCTCTGCT CCTGCCACTTCCCCTGCTGTCGCCTTAGCACCATGGAAGCCACCAAGCAAGTGGTTAACTTTGGGCCCGG GCCTGCCAAGCTGCCACACTCGGTATTGTTGGAGATCCAGAAGCAGCTACTAGACTACAGAGGACTCGGC ATCAGTGTGCTCGAAATGAGTCACAGGTCATCAGATTTTGCCAAGATTATTGGCAATACAGAGAATCTTG TGAGGGAATTGCTAGCTGTTCCCAACAACTACAAGGTGATCTTTGTACAAGGAGGTGGGTCTGGCCAGTT CAGTGCTGTCCCCTTAAATCTGATTGGCCTGAAAGCTGGAAGGAGTGCTGACTACGTGGTGACCGGAGCT TGGTCAGCTAAGGCTGCGGAAGAAGCCAAGAAGTTTGGAACGGTGAACATTGTCCACCCTAAACTTGGAA GTTACACAAAAATTCCAGACCCAAGCACCTGGAACCTCAACCCGGACGCCTCCTATGTATACTTCTGTGC AAACGAGACTGTGCACGGGGTGGAGTTTGACTTCGTACCTGACGTCAAGGGAGCGGTGCTGGTCTGTGAC ATGTCCTCAAACTTCTTATCCAGGCCGGTGGATGTTTCCAAGTTTGGTGTGATTTTCGCTGGTGCTCAGA AGAATGTTGGCTCTGCCGGGGTGACGGTGGTGATTGTCCGGGATGACCTGCTGGGGTTCTCGCTCAGAGA GTGCCCATCAGTCCTTGACTACAAAGTGCAGGCTGGGAACAACTCTTTGTATAACACACCTCCGTGCTTC AGCATCTACGTCATGGGCATGGTCCTGGAATGGATCAAGAACAACGGCGGGGCCGCAGCCATGGAGAAGC TCAGCTCCATCAAATCCCAAATGATTTATGAGATCATTGATAATTCTCAAGGATTTTATGTATGCCCAGT GGAGCGCCAGAATAGAAGCAGGATGAACATCCCATTTCGCATTGGCAACGCCAAAGGAGACGAAGCTTTG GAAAAGCGGTTTCTTGACAAGGCTGTAGAACTCAACATGATCTCCTTGAAGGGGCACAGGTCAGTGGGAG GCATTCGTGCCTCTCTGTATAACGCTGTCACAACCGAAGACGTTGAGAAGCTGGCGGCCTTCATGAAGAA TTTCTTGGAGATGCATCAGCTGTGAACATGACCAGCCACGCTCTGCCTGTGAGCAGCACGCAGCGTCATT TAGAGTAACTTGGGATTGACTACAGAAACTTAACAGACATGGATTTTTTTTTCCCAAATACATGCTTATT ACAGATTCCTTTCTCAAAGAATAACGACTAAACATCAGGGCACTGCAGAGCTGAGGAAACTCCCAGCTAA CAGCCACCTTCATTCTCATGTGACCTGGAAGCATTTTGAAAGCTTTCTTTCCTGTTGCTCTTCTAAATGA ATTCAGCCTGTCAGCCATCTTTGCTGCCCCCTTCTCTAGGTGGACTGCCTTGGGCTTTGCCCTGCTGGTT CCAACTGGTTATTATTAGGAGGTTGGAGGCCCTGTGGGCTGGATTGACAGCAGTGAGTTTGCTGGGTTCT GCAGCAGTTGGTTCAAGTCAACAGCGACTCACGAGCGTTTTGGATCTGTGTACTGCAATTCCCATAAAGA GATAGGTGTGTTATGCTGTGTTTATGTAGTGGGTATTGTTTTAAACACCGTACGGTTTATGCCTATGCCT TTATATTTCTAATGAAACAGTTTTGTTCAGCCCATGACTTTGTGCATGACTGTCGTCCGTTCTAGTCACC TGTGCTCTCCATCTACTGCCTTTTAAAGCTGCGTCATGAGAAGGATCTACACGTTCCACCATGACTCTCG TTTCTTGCCACAAGTAGAAGAAATGGTTGATTCTTTGCTTTTTGGTTAGGCCGTTAAACAAAACTCAGTC ACACCCCTGCCTTCCACCCTCAAACTGTGATCACGTGGCTGTTCTTGTAGTTTAACCAGGGCAACACTAT ATATTCCAGGTCTATACATTGCTGAGGTCTTTTTCTGGTTGTTCATGTATCAGTGTTTTCAATCGACTGC ATGGAAAACATTAAAGTCCTAAGTCATCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2616298 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2616298 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17270 same embryo
	EMAGE:17267 same embryo
	EMAGE:17269 same embryo
	EMAGE:17265 same embryo
	EMAGE:17268 same embryo
	EurExpress:euxassay_001596 same experiment
	MGI:4827464 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source