EMAGE mouse gene expression spatial database

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EMAGE:17270

Exoc6 exocyst complex component 6 ( MGI:1351611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001597. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001597_01	euxassay_001597_02	euxassay_001597_03	euxassay_001597_04

euxassay_001597_05	euxassay_001597_06	euxassay_001597_07	euxassay_001597_08	euxassay_001597_09

euxassay_001597_10	euxassay_001597_11	euxassay_001597_12	euxassay_001597_13	euxassay_001597_14

euxassay_001597_15	euxassay_001597_16	euxassay_001597_17	euxassay_001597_18	euxassay_001597_19

euxassay_001597_20	euxassay_001597_21	euxassay_001597_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17270_wholemount_strong.wlz

17270_wholemount_moderate.wlz

17270_wholemount_weak.wlz

17270_wholemount_possible.wlz

17270_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17270_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11

diencephalon

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 12 13 14 15 16 17 18 19 20 21 22

hindbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 14 15 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 14 15 16 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 06 14

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 13 14 15

tongue

moderate

regional

moderate expression: see section 10 weak expression: see section 09 11

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

moderate

regional

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5684
Entity Detected:	Exoc6, exocyst complex component 6 ( MGI:1351611)
Sequence:	sense strand is shown >T5684 GCCAAGATGGCGGAGAGCTGCGAGGCTCTGGGCACGGTCCCGGAGCACGAGCGGATCTTGCAGGAGATCG AAAGCACCGACACCGCCTGCGTAGGGCCCACCCTCCGATCTGTGTATGATGATCAGCCAAACGCACACAA GAAGTTTATGGAAAAGCTAGACGCTTGTATCCGCAATCATGACAAGGAAATTGAAAAAATGTGTAACTTC CATCACCAGGGCTTTGTGGATGCGATTACGGAACTCCTCAAAGTAAGGGCCGATGCGGAAAAACTGAAGG TTACCGATACCAACCGAAGGTTTCAAGATGCAGGAAAAGAGGTGATAATCCAAACAGAAGATATTATTCG GTGTAGGATTCAGCAGAGAAATATTACAACCGTAGTAGAGAAATTACAGTTATGCCTCCCAGTGTTGGAG ATGTACAGTAAGCTGAAGGAACAGATGAGCATGAAAAGGTATTACTCCGCACTTAAAACAATGGAACAGC TAGAGAATGTGTACTTTCCCCGGGTCAGCCAGTACCGCTTCTGCCAGCTCATGATGGAGACACTCCCTAA ACTCCGGGAAGACATGATGAACTACTGTATGTCTGATCTCACGTACGGCCTGGAAAGCATCCGAAAGCAT TCTGACAAAATAGGTGAAGCAGCCATGAAGCAGGCACAACAGCAGAAGAGCTTCAGTGTTGCTCTGCAGA AGCAAAATAATATGAGGTTTGGGAAAAACATGCATGTAAATAATGACAGGATTCTAGAAGAGAAGAGTGA TGTTATACCGAAGCATGCACTTGAAGAGGAGGCGGAGAACGACGAGGAGGTCTTAACTGTTCAAGATCTC GTTGATTTTTCCCCTGTTTACCGATGCTTGCACATTTATTCTGCTTTGGGTGATGAAGAAACATTTGAAA ATTATTACCGGAAGCAAAGGAAGAAGCAGGCACGCCTGGTTCTGCAGCCACAGTCGAGTGTGCATGAAAC AGTTGATGGCTATAGAAGATATTTCACTCAAATTGTAGGGTTCTTTGTTGTGGAAGATCACATTTTACAC GTGACCCAAGGATTAGTAACCAGGGTCTACACTGAGGAACTCTGGAACATGGCCCTCTCAAAGATAATTG CTGTCCTCAGAGCTCACTCATCTTACTGCACTGACCCTGACCTTGTTCTGGAGCTGAAGAATCTCATCGT AATTTTTGCAGACACTCTGCAGGGTTATGGCTTCCCAGTCAACCGGCTCTTTGACCTTTTATTCGAAATA AGAGATCAATATAACGAAACACTGCTGAAGAAGTGGGCTGGTATTTTCAGGGACATTTTTGAAGAAGATA ATTACAGCCCCATCCCTATTGGCAGTGAGGAAGAATATAAAGTAGTCATCAGTAGATTCCCCTTTCAGGA TCCAGACCTCGAAAAGCAATCTTTCCCAAAGAAGTTTCCTATGTCCCAGTCTGTGCCTCTTATTTATATT CAAGTTAAAGAATTTATTTATGCCAGTCTTAAATTTTCAGAGTCGCTACACCGAAGCTCAACAGAAATAG ATGACATGCTTAGGAAATCAACCAATCTGCTGCTGACCAGAATTCTGAGTAGCTGTTTACTGAACCTTAT TAGAAAACCTCATATAGGTTTGACAGAGCTGGTGCAAATTATCATAAACACAACACACCTGGAACAAGCT TGCAAATACCTAGAGGACTTTATAACTAACATTACAAATATTTCTCAAGAAACCGTTCACACCACAAGAC TTTATGGACTTTCTACTTTTAAGGATGCTCGACATGCGGCAGAAGGAGAAATATATACCAAACTCAATCA AAAAATTGATGAATTTGTTCAGCTGGCTGATTATGACTGGACCATGGCTGAGTCAGATGGGAGAGCAAGT GGTTATTTAATGGATCTTATTAATTTTCTGAGAAGCATCTTTCAAGTGTTTACCCATTTGCCTGGGAAAG TTGCCCAGACAGCTTGCATGTCAGCCTGCCAACATCTGTCCACATCCTTAATGCAGATGCTACTGGACAG TGAGTTAAAGCAGATCAGCATGGGAGCCGTGCAGCAGTTTAACCTCGATGTCATACAGTGCGAATTGTTT GCCAGCTCTGAGCCTGTGCCAGGATTCCAAGGGGACACCCTGCAGCTGGCCTTCATTGACCTCAGACAGC TCCTTGACCTGTTTATGGTTTGGGATTGGTCTACTTACCTGGCTGACTATGGGCAGCCGGCTTCCAAGTA CCTCCGAGTGAACCCACACGCAGCCCTCACTCTTTTGGAGAAGATGAAGGACACCAGCAAGAAGAATAAC ATATTCGCCCAGTTCCGGAAGAATGACCGAGACAGACAGAAGCTGATAGAGACAGTGGTGAGACAGCTCA GAGGCTTGGTGACTGGTATGTCCCAGCACACGTAGACCAGACCCATGCCTGAGTGCTTCTCTGTGGCCAG CACTGGTCGTGAGACAATAACTTGTTTACAGAAGCCAAAAACTGTAGTAGAGGAAAGACACTGATGAGGG CTTAAACAAGCAATGGCGGCAAACATCATTTGTATGGATTTTTAAATGACTGATTACTATTTTATATTTA AAAATATAACAACTTTTTTCAATTTTAGAAGAAAAGTCAAAGTGTAATATATAATCCCAATAAATTGTGT ACAAAACTGATTGCAAATATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2631201 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631201 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17266 same embryo
	EMAGE:17267 same embryo
	EMAGE:17269 same embryo
	EMAGE:17265 same embryo
	EMAGE:17268 same embryo
	EurExpress:euxassay_001597 same experiment
	MGI:4824646 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source