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EMAGE:17342

Vill villin-like ( MGI:1201781)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342
"Pseudo-wholemount" of euxassay_003905. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003905_01 euxassay_003905_02 euxassay_003905_03 euxassay_003905_04
EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342
euxassay_003905_05 euxassay_003905_06 euxassay_003905_07 euxassay_003905_08 euxassay_003905_09
EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342
euxassay_003905_10 euxassay_003905_11 euxassay_003905_12 euxassay_003905_13 euxassay_003905_14
EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342
euxassay_003905_15 euxassay_003905_16 euxassay_003905_17 euxassay_003905_18 euxassay_003905_19
EMAGE:17342 EMAGE:17342 EMAGE:17342 EMAGE:17342
euxassay_003905_20 euxassay_003905_21 euxassay_003905_22 euxassay_003905_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17342Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17342_wholemount_strong.wlz
17342_wholemount_moderate.wlz
17342_wholemount_weak.wlz
17342_wholemount_possible.wlz
17342_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17342_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23
cervical intervertebral disc
moderate moderate
regionalmoderate expression: see section 07
radius
moderate moderate
regionalmoderate expression: see section 23
ulna
moderate moderate
regionalmoderate expression: see section 23
humerus
moderate moderate
regionalmoderate expression: see section 03 04 05 18 19 20
hand
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 18 19 20 21 22
foot
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 18 19 20 21 22
leg
moderate moderate
regionalmoderate expression: see section 21
fibula
moderate moderate
regionalmoderate expression: see section 21 23
tibia
moderate moderate
regionalmoderate expression: see section 21 23
femur
moderate moderate
regionalmoderate expression: see section 06 07 20 22
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 12 13
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 15 16 17
pancreas
moderate moderate
regionalmoderate expression: see section 07 08 09
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 20
4th ventricle
moderate moderate
regionalmoderate expression: see section 11 12
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 14 15 16 17
otic capsule
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 21 22
cochlea
moderate moderate
regionalmoderate expression: see section 07 16 17
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 20 21 22 23
naris
moderate moderate
regionalmoderate expression: see section 10 14
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
nasal septum
moderate moderate
regionalmoderate expression: see section 12
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
esophagus epithelium
strong strong
regionalstrong expression: see section 11 12
pharynx epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08
rectum
strong strong
regionalstrong expression: see section 11
midgut
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15
palatal shelf
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
bladder
moderate moderate
regionalmoderate expression: see section 10 11
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 07 08 14 15 16 17 18
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 11
urethra of male
moderate moderate
regionalmoderate expression: see section 11
lung
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 10 13 14
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 08 10 11 12 13
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 07 08 10 11 13 14
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22
sternum
moderate moderate
regionalmoderate expression: see section 11
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7002
Entity Detected:Vill, villin-like ( MGI:1201781)
Sequence:sense strand is shown

>T7002
GGAAGGTGCAGAGGCAGTTCCCAGAAGCCCAGGATGGACATCAACCAGGACCTCCCAGCCATCGACAGCC
ACAGAGCCTTGCAAATATGGATTACTGAGAACCTAAAGATGCTGCCATTGCCTGAAAGGGCTCATGGGAA
TTTCTTTGAAGAATGCTGCTATGTCGTCCTTCATGTCCCTCAGAGTCCAAAAGCTACCCAGGGAGGATCC
AGTGACCTGCACTACTGGATTGGAAAGGATGCCAGCGCAGAGGCCCGGGAGGCTGCGGTCTCCTTTGTGC
AAAGTCTGCAGGAGGACCTAGGAGACCAGACTGTGCTGCACCGAGAGTCACAGGGCCACGAGTCCGACTG
CTTCCACAGCTATTTCCACCCTGGAGTCATCTACAGGAAGGGAGGCAGAGACTCTGCTCTCAAGCTTGCG
GAGACCAACATGTACAATGTCCAGCGACTGCTTCACATCAAGGGAAGGAAGCACGTGTCTGCCACTGAGG
TGGCCTTGTCCTGGAACAGCTTTAACAAGGGGGACATCTTCGTGTTGGACCTGGGCAAGGTGATGATCCA
GTGGAATGGACCCAAAGCCAGCATTTCTGAGAAAGCACGGGCTCTGACCCTGACTTGCAACCTCAGAGAC
AGGGAGCGAGGTGGCCGTGCCCAAATTGCTGTGGTAGATGCGGAGAATGAAGCCACCAACCTTCTGCGCA
TCATGGAGGCTGTGCTGGGCTGCAGATCAGGCAGCCTGTGTCCCTCTGTACCCAGCAACAGTGTTAGCCA
GCTACAAAAGGCCAATGTCCGCCTCTACCATGTCTGTGAGAAGGGGACAGACCTGGTGGTCCAGGAACTG
GCGACCCGCCCACTGACCCAGGACCTCCTGCAAGAGGACGGTTGCTATCTCCTGGACCAGGGTGGCTTCA
AGATCTACATGTGGCAGGGACGAAAGTCCAGCTCCCAGGAGAAGAAGGCTGCCCTCAGCAGGGCTGTGGG
CTTCATCCAGGCCAAGGGTTACCCGAACTACACCAATGTGGAGGTGGTGAACGACGGCGCGGAGTCCACA
GCTTTCCAACAGCTCTTCTGGTCGTGGTCGAAGGAGCTAGATAGGAAAAAACACCCAGAGAAGAGTAAAC
TGGTGCAGGGAAACCTGGAGGTAGGCAAGCTTCACACCCAGCCTGAGCTCGCGGCCCAGCTCAGAATGGT
GGACGATGGCTCTGGGAAGGTGGAGGTGTGGTACATCCAGGACTTACAAAGACAGCCTGTAGATCCCAAG
CACTATGGCCAGTTGTGCTCAGGAAACTGCTACCTTGTCCTCTACACATACCAGAAACTGGGCTGTGTCC
AGTACCTCCTGTACCTATGGCAGGGCCACCAAAGCACTGTAGAAGACACCAAGGCCCTGAACTGCAGTGC
TGAAGAGTTGGACCTCATGCACCAGGGTGCACTGGCGCAGGGGCATGTGACCATGGGCAGTGAGCCTCCC
CACTTCCTAGCCATCTTCCAGGGGCGGCTGGTGGTCTTCCAGGGGAATGCAGGCAACAAAGGGGAAAGAC
CGCCAGTATCCGACACCAGGCTTTTCCACGTGCAAGGGACCGAGAGCCACAACACCAGAACTATGGAGGT
GCCGGCCCGTGCCTCCTCCCTCACTTCCGGTGACGTCTTCTTTCTGATCACAAGTCATGTTTGCTATCTC
TGGTTTGGGAAGGGCTGTCATGGGGACCAACGTGAGATGGCGCGGACGGTGGTCACTGTCTTCCCAGGGA
ATAACAAGGAGACAGTGCTGGAGGGTCAGGAGCCTCTCTACTTCTGGGAAGCCCTCGGAGGCCGGGCCCC
CTATCCCAGTAACAAGAGGCTTCCCGAGGAGGTGTGGAGCATCCAGCCCCGACTGTTCGAGTGCTCCAGT
CACACAGGCTGCCTGGTCCTCACGGAAGTGCTGTTCTTTGGCCAAGAGGACTTGGACAAGTATGACATCA
TGTTACTAGACACCTGTCAGGAGAACAGCCAGTCCTACGAGGAGATGGTGGGGAACAGCCTGGGACCGGG
ATCTGCAATCTCTGAGATGACAGCAGCCTTCAAGGGTTCCCAGGACAGTCCAGAGAATGAGCTGGGGCTG
GACCTCAGGGTGGATGGCGCGAACCCCAGCATGAACCACACCAGCAGCTGCAGTGACTCAATGGTCAATG
GGAGCCTGCCCCGGGAAAGGCTGATGCACCAGGCACTGGAGGACCTGCCACCGGGTGTGGACCCGGCCCG
TAAGGAGTTCTATCTCTCAGACTCCGATTTCCAAGACATCTTCGGGAAATCCAAGGAGGAGTTCTACAGC
ATGGCCAAGTGGAAGCAGCAGCAAGCGAAAAAGAAGCTGGGCTTCTTCTGATGTGGGGAACCCTGCGCCA
CATACCCTGAAATTCCTCCTGGGGTAACTCCCACTTCCTAATAAAAGCCAGTGGCTGGCAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3991439 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3991439 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17341 same embryo
 EMAGE:17343 same embryo
 EMAGE:17340 same embryo
 EurExpress:euxassay_003905 same experiment
 MGI:4829160 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS