EMAGE mouse gene expression spatial database

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EMAGE:17379

Mapk15 mitogen-activated protein kinase 15 ( MGI:2652894)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003819. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003819_01	euxassay_003819_02	euxassay_003819_03	euxassay_003819_04

euxassay_003819_05	euxassay_003819_06	euxassay_003819_07	euxassay_003819_08	euxassay_003819_09

euxassay_003819_10	euxassay_003819_11	euxassay_003819_12	euxassay_003819_13	euxassay_003819_14

euxassay_003819_15	euxassay_003819_16	euxassay_003819_17	euxassay_003819_18	euxassay_003819_19

euxassay_003819_20	euxassay_003819_21	euxassay_003819_22	euxassay_003819_23	euxassay_003819_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17379_wholemount_strong.wlz

17379_wholemount_moderate.wlz

17379_wholemount_weak.wlz

17379_wholemount_possible.wlz

17379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22 23 24

upper arm

moderate

regional

moderate expression: see section 22

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 18 19 20 21 22 23

upper leg

moderate

regional

moderate expression: see section 22

diencephalon

moderate

single cell

moderate expression: see section 13

telencephalon

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 18 19

choroid invagination

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 17 18 not examined expression: see section 05

4th ventricle

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 14 15 16 17 18 19 20 21

medulla oblongata

moderate

single cell

moderate expression: see section 08 17

metencephalon

moderate

single cell

moderate expression: see section 06 07 08 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 weak expression: see section 06

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13

nasal septum

moderate

regional

moderate expression: see section 11

viscerocranium

weak

regional

Expression in the turbinate bone.

metanephros

moderate

spotted

moderate expression: see section 06 07 08 09 15 16 17 18 19

orbito-sphenoid

weak

regional

weak expression: see section 01 02 04 05 06 17 18 19 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7759
Entity Detected:	Mapk15, mitogen-activated protein kinase 15 ( MGI:2652894)
Sequence:	sense strand is shown >T7759 CGCGGCTGCTGGTTCCCAAGCCAACGGATCAACAGGTCCAGCTCCAGAGAAAGAATCGGTGGACCCCTAG AGCCAGCGCCTGCCTCTCGCCATGTGTGCTGCCGAGGTGGACCGTCATGTAGCCCAGAGATACCTGATCA AGCGGAGGCTTGGGAAGGGGGCCTATGGCATTGTGTGGAAGGCCATGGACCGGAGGACTGGCGAGGTTGT GGCCATCAAGAAAATCTTTGATGCCTTTAGGGACCAGATAGATGCTCAGAGGACCTTCCGTGAGATTATG CTTCTCAAGGAGTTTGGAGGCCATCCCAACATCATCCGCCTGCTTGATGTAATCCCAGCGAAGAATGACA GGGATATTTACCTGGTGTTTGAGTCCATGGACACCGACCTGAATGCAGTCATCCAGAAGGGCAGACTACT GAAGGACATCCACAAGCGGTGCATCTTTTACCAGCTCCTGAGAGCCACCAAGTTTATCCATTCAGGGCGC GTCATCCATCGGGATCAGAAGCCAGCCAATGTTCTACTGGATTCTGCTTGCCGGGTGAAACTCTGTGACT TTGGCCTGGCACGCTCCCTCGGTGACCTCCCTGAGGGGCCTGGGGGTCAGGCCCTGACAGAGTATGTGGC CACACGCTGGTACCGAGCTCCAGAGGTGCTTCTGTCTTCCCGATGGTATACCCCTGGGGTGGACATGTGG AGCCTGGGCTGCATATTAGGAGAGATGCTTCGAGGGCAGCCACTGTTTCCCGGGACATCTACTTTCCACC AGCTGGAGCTGATCCTGAAGACCATCCCATTGCCTTCCATGGAGGAGCTCCAGGACCTTGGCTCAGACTA CAGTGCTTTGATTCTGCAGAATCTTGGGTCCAGGCCACAGCAGACGCTGGACGCCCTCCTGCCGCCAGAC ACCCCCCCAGAAGCCCTGGACCTCCTCAAGCGACTCTTGGCGTTTGCTCCGGACAAGCGCCTTAGTGCAG AGCAGGCGCTGCAACACCCCTACGTGCAGAGATTCCATTGCCCCGACCGCGAGTGGGCACGGGAGTCCGA CGTGCGGCTCCCGGTGCACGAAGGAGACCAGCTCTCTGCACCAGAGTATCGCAAACGCCTGTACCAGATT ATCCTGGAGCAAAGTGGGAACAGCCGCAGCCCTCGAGAGGAAGGCCTGGGGGTTGTGGCCTCGCGGGCTG AGCTCAGGGCTTCCCCGGCCCGGACGCAATCGCTCAAGTCGGGAGTCCTCCCCCAGGTCCCGGCGGAGAC GCCAGCGCGAAAACGCGGACCCAAACCTCCGCGTAGCCCTGGTCATGATCCTGAGCATGTGGAAGTTCGC AGGCAGAGCTCAGACCCCCTGTTCCAACTTCCGCCGCCAGGAAGGGGGGAAAGGCCCCCAGGGGCCACAG GGCAGCCACCCTCGGCACCCTCAGGGGTGAAGACTCAAGTGAGGGCGATGGCGCCGTCCCTGACTTCACA GGCAGAGGCTCAGGCGGCCAATCAGGCTCTGATCCGCAGTGATCCGGCCCGGGGCGGTGGGCCGAGGGCG GTCGGCGCGCGACGGGTCCCTTCCCGCCTGCCCCGGGAGGCCCCGGAACCCCGACCCGGCCGAAGGATGT TTGGCATCTCGGTCTCGCAGGGGGCCCAGGGCGCAGCCAGAGCTGCGCTTGGCGGCTACTCCCAGGCCTA CGGGACCGTGTGCCGCTCGGCGCTGGGCCGCCTGCCCCTGCTCCCCGGACCGCGTGCGTGAGCCACCCAC CCACCTCCTTGAGGCAACCCGGCGCCGTCCCCCAGACCCCAATGCATCTTCTCCCTCCTCTGCCTCCCTG CCTTCGAGTTCCCGGAGGGTTCTTTGAGCCTCTCTCCCCTAAGAAAGAGTAGATGGAAGTCTCCACTCCA CCCCCCAATACTTTCTTCCAATAAAGTTTTATCTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6312082 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312082 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17383 same embryo
	EMAGE:17382 same embryo
	EMAGE:17380 same embryo
	EMAGE:17381 same embryo
	EMAGE:17378 same embryo
	EurExpress:euxassay_003819 same experiment
	MGI:4826090 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source