EMAGE mouse gene expression spatial database

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EMAGE:17382

Nhlh1 nescient helix loop helix 1 ( MGI:98481)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003709. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003709_01	euxassay_003709_02	euxassay_003709_03	euxassay_003709_04

euxassay_003709_05	euxassay_003709_06	euxassay_003709_07	euxassay_003709_08	euxassay_003709_09

euxassay_003709_10	euxassay_003709_11	euxassay_003709_12	euxassay_003709_13	euxassay_003709_14

euxassay_003709_15	euxassay_003709_16	euxassay_003709_17	euxassay_003709_18	euxassay_003709_19

euxassay_003709_20	euxassay_003709_21	euxassay_003709_22	euxassay_003709_23	euxassay_003709_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17382_wholemount_strong.wlz

17382_wholemount_moderate.wlz

17382_wholemount_weak.wlz

17382_wholemount_possible.wlz

17382_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17382_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 08 09 10 11 moderate expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 12 13 14

cerebral cortex marginal layer

strong

regional

strong expression: see section 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cerebral cortex ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05

olfactory cortex marginal layer

strong

regional

strong expression: see section 08 09 10 13 14 15

hindbrain meninges

strong

regional

strong expression: see section 06 07 08 16 17 18 moderate expression: see section 09 10 11 14 15

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 20 21 moderate expression: see section 09 10 11 15 16 17 18 19

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 20 21 moderate expression: see section 09 10 11 15 16 17 18 19

pons ventricular layer

strong

regional

strong expression: see section 07 08 moderate expression: see section 06 09 10 14 15 16 17 18 not examined expression: see section 12

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 17 18 19 weak expression: see section 05 06 07 08 16

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 moderate expression: see section 07 14 15 16

not examined

regional

not examined expression: see section 01 02 21 22 23

neural retina

moderate

single cell

moderate expression: see section 01 02 21 22 weak expression: see section 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7758
Entity Detected:	Nhlh1, nescient helix loop helix 1 ( MGI:98481)
Sequence:	sense strand is shown >T7758 GTGAGTGTGTGAATGTGTAGGTGTGTGTATGTATGTGTGAGTGTGGGTGACTCCAGTTCTGGACTAAGTA AGAGCATCCCTTTTGCCTAGGCCTTACAAATCCTCATCATGTGTACCCTTCTCATCTGTCCTGGATCCTG TTCAGCCACAAGCTGCCCCTAAGAGCTCTAGAAACTGCTCACTAACTTTGCAGACAGATGACCCTTGAGC AGCGAGAAGAGACTGGGGCTCTCTACGCTGTCCCTCTCACTTGCCAGCTTGGATCCCTTGGCAGAGTCCT TCTAGGCTCCAGGCTGGAATCTTGACCATGGAACTTTGAAGTAACTGCTTCCCAGAGCTCAGTGGCAGAC CCCGGCACTCCAGGCCCTTGCTGCTTTCCTCCTAACCCTCTTTGAAGCTCCTAAAGGGAGGGGTGGGGAG AGGATCCGCATTTCCCTAGGTGGGGGGTTTTCCACCATGATGCTCAACTCCGATACCATGGAGCTGGACC TGCCTCCCACCCACTCGGAGACCGAGTCGGGCTTTAGCGACTGTGGGGGCGGACCGGGCCCCGATGGTGC TGGATCCGGGGATCCAGGAGTGGTCCAGGTCCGGAGCTCAGAGCTTGGAGAGTCCGGCCGCAAAGACCTG CAGCACTTGAGTCGTGAGGAGCGCAGGCGCCGGCGCCGCGCCACGGCCAAGTACCGCACGGCACACGCCA CGCGGGAGCGCATCCGCGTGGAAGCCTTCAACCTAGCCTTCGCCGAGCTGCGCAAGCTGCTGCCCACTCT GCCCCCGGACAAGAAGCTCTCTAAGATTGAGATCCTACGCCTGGCCATCTGCTATATCTCCTACCTGAAC CATGTGCTGGACGTCTGAACTCAGCCCGCATCCCACCCTGGATTCTCCCCATTTCCCTGGGCCTCTCCAG AGCCCCCTGTCACCATACATTACTTAGAATGGCCGGCCCCCTCCCATCCCAGAGGACCAGGCTCACATCG CTAGTCCTGAAGGCGGTTTCTTTTCATTGGCCCAGGAATGTGAAGGATGTTCTTATGGGTTCCTTCAGAG AGTTGTTCCGGCCTAGCTTGGTCAGAGTTGCTTGCCAGAGTGGTCAGAATGGAGTCCGTGCTGGGTTTAC TAAGCCTAGCCTCTCTGCACAGCTGCTGCCTCATCTATCCTATGCCCAAGTTTTCAGAGACCCCAGACCC TAACTCTCTTTCTGGCAATGGTGGGACTCAAAATCGGAATGGGAACTGACTAGCGGCAGAGGTTGAAGGA GCGGGTCCCCAACTCCCAGACTTTTCGGTCCATTGGGGGGGGGGCAGTGGCGGGGGTGGGGGGGGGGGAG GAATGGGCTCTTGGGATGGCAGAGAGATGACGGAGGTGAATCCTGGGTAGCCAGGGATTATAGGAAAATT CTGCCTTTCTTTTGTCTCTCTCAGTCCTGAGGCTTAGGATATACAGACCTCAGATCTAACTTGGTAGTGA GTGCCTTGCCTTCTTGGAGCTGTCCGGCCGCTCCCGGTCCCCTCCCACCCCTGCCACCCAGAATTCTCCC TTTCCCTGTGCCGGCTAGAAAAAGGAAAACCTTAGTCCTGGGATAAGGATGACACCCCCAAACAGCCCAG GGCACCTTGCAGAAGGCTCAGGCCCTGGGTGGGGCCGCTCCACAGCAGCTCCTTCCATCCCAGGGGACCC TTGAAAACATGCAAACCCCATCAGCTCCTCCCTCAGATGCAAGGGCATGCCTGCTACTGCCCTCTACGAA TGCTCTAGGGCCTTCTTATTACATTTTTCCCCTTCCTTTCTTAAACCGCCGTGGGAAGGAAGGTGACCCG CCCATGCTGCTGAGCCCCACCCACCTGCCCATTGTAGCCTTGCGACCCAGAGTCTATGGGCCTTAAATTC CCTGTAGAAGAACTCACTGCTTTTCTCTGCTCCATCCATCCTCACACCAAACTCCTAGCTCTACAAGGAT ATTTATTTATGTATTTATTTATTCACTTATTTATTTATGTATTTATTTATTTATAAATATTACTATTTAT TACCGAGTTATGCACTTTGGGGTAGGGTGAGGGGGGCTCCTTGCAGCTTGCTTTAGCTGAGGTCCTCTTG CTCTCTCCCGGGTCACTTCTCTTCTTCTTTCTCTAGTGCAAGTATGTGTGGGGATCCCTTCACCCACCAT ACTGGTACCCCTTCTTCAGCTCCATCTGTCCCTATCCCCTTTCCTCTGGAATAGTGTCCCTTCTCAGCCT CCCAGCTTCTAGGGGGTCCTTTCTCAATCTCTCCCTGCTGCCCCCACCCCGACAATCCCCCTCCAGTCCT GTGTACTCCATTCCTCTTACCAGCCCTGCTTTTCCCCAAACCACCAAGAGCAGACCCTGGGATCTGTGTC TGGTGTCCTGTGTGTCTTTGTCTGGTTGCATTCCTAATTTCCTACAAAAAGAAAAATTAAAGTGACCTCG ATCTAGTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312358 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17383 same embryo
	EMAGE:17380 same embryo
	EMAGE:17381 same embryo
	EMAGE:17378 same embryo
	EMAGE:17379 same embryo
	EurExpress:euxassay_003709 same experiment
	MGI:4826704 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source