EMAGE mouse gene expression spatial database

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EMAGE:17383

Elavl2 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) ( MGI:1100887)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003701. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003701_01	euxassay_003701_02	euxassay_003701_03	euxassay_003701_04

euxassay_003701_05	euxassay_003701_06	euxassay_003701_07	euxassay_003701_08	euxassay_003701_09

euxassay_003701_10	euxassay_003701_11	euxassay_003701_12	euxassay_003701_13	euxassay_003701_14

euxassay_003701_15	euxassay_003701_16	euxassay_003701_17	euxassay_003701_18	euxassay_003701_19

euxassay_003701_20	euxassay_003701_21	euxassay_003701_22	euxassay_003701_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17383_wholemount_strong.wlz

17383_wholemount_moderate.wlz

17383_wholemount_weak.wlz

17383_wholemount_possible.wlz

17383_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17383_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 08 09 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 11 13 14

cervical ganglion

strong

regional

strong expression: see section 08 09 15 16

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 15 16 17

neural retina

strong

regional

strong expression: see section 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16

esophagus

strong

regional

strong expression: see section 12

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

not examined

regional

not examined expression: see section 08 09 10 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7754
Entity Detected:	Elavl2, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) ( MGI:1100887)
Sequence:	sense strand is shown >T7754 GCCGCCGCCAGCGTGACTCCTCTGAGCGGTGCTGTCGGCCGGCGGCGCGAGCCGTGGGAGCTGCAGCCGA GCCGCAGCAGCAGGTAATTGCTGCCATGGAAACACAACTGTCTAATGGGCCAACTTGCAATAACACAGCC AATGGTCCAACCACTGTAAACAACAACTGCTCCTCACCAGTTGACTCCGGGAACACAGAGGACAGCAAGA CCAACCTAATAGTCAACTACCTTCCTCAGAACATGACACAGGAAGAACTAAAGAGTCTTTTTGGGAGCAT TGGCGAGATAGAGTCCTGTAAGCTTGTAAGAGACAAAATAACAGGGCAGAGCTTGGGTTACGGATTTGTG AACTACATTGACCCCAAGGATGCAGAGAAAGCTATCAACACTCTGAATGGATTGAGACTTCAAACCAAAA CAATAAAAGTTTCCTATGCTCGCCCAAGCTCAGCCTCAATCAGAGATGCAAACTTATACGTCAGCGGGCT TCCAAAAACCATGACCCAGAAGGAGCTGGAACAGCTGTTTTCACAGTATGGGCGCATCATTACTTCTCGG ATTCTTGTCGACCAGGTCACTGGCATATCAAGGGGTGTAGGGTTTATTCGATTTGACAAGCGGATTGAGG CAGAAGAAGCTATCAAAGGCCTAAATGGCCAGAAACCTCCTGGTGCCACAGAGCCAATCACTGTAAAGTT TGCTAATAATCCAAGCCAAAAAACCAATCAGGCCATCCTTTCCCAGCTGTACCAGTCTCCAAACAGAAGG TACCCAGGACCGCTAGCTCAGCAGGCACAGCGTTTTAGTAGGTTTTCTCCAATGACGATCGATGGAATGA CCAGTCTGGCTGGAATTAATATTCCTGGGCACCCTGGAACAGGCTGGTGCATATTTGTGTACAACCTGGC GCCTGATGCAGATGAAAGTATCCTGTGGCAAATGTTTGGGCCTTTTGGAGCTGTCACCAACGTGAAGGTC ATCCGTGACTTTAACACCAATAAGTGCAAAGGTTTTGGATTTGTGACTATGACAAACTATGATGAGGCGG CCATGGCGATAGCTAGCCTCAATGGATACCGCCTGGGAGACAGAGTCCTGCAGGTCTCCTTTAAGACAAA CAAAACGCACAAAGCCTAATGAGCTCTTGTCCTCAGTCCATTTATATATGAAAACTATACAACAAAAGCA AGTTAAGAGAAACTTTATACATTAGTAAATGTCTTTGTAAGTCAGTGTTGAGATGGGGATAAATGACTAC TTAGCATCCTAAGAAATATGTGAGATTTTTTATTGCTAGTATTTGAATTAAAACTTCTTAAATATCTTTT ATGTTTGAATATGGACAAGAGGTACAGGGTTTTTACCTGTCACATTGCATTCTATTGCCTTCTTTGAAGA AGGTGGACCTTTTAAAGTGTTTCAGCTAAGGGAAGACATTTCTTTTCTTTTTACATAACTGCCTTGATCC TGTGAGTAAGTACTGAGGCTTTGTGTTGTAGCTCTTCAGTTGGTTGTCTTTCCCCACCTCCTTATCTTTC CTTTTCTTTCTCTTTCTTTTGTCTTTGTTTTTTCCTTTCCATTCTTTAATTAGCTTTGTGTTTGGTTTAC ATTTCAAGCATTGCTGTTATGTCTCAGAAAAGCATTTTGAAGTTTACATTTTTATTTATGAAGTTACAAA CAGTATTTATTTTGTAATTCTGATTTGGGCTGGGGCAGGGGGGGCGACATTATAAACGCTTATTGTAAGA ATACTGGAGAACTTTTCGTAAAGCAGTACCTTGCCAAAGAGATAAGAGCCTCTTTGATGTGGGTTTAAAA AAAGCATCTATTTTTATAAAAAAGAAAATTTGGAGAAACTTTTTACTGGTCCTGGAACAAATATTTTGAC TTGAATACTTTGAGAAATCTCTTCATATGACACCTAGTGAGCTTTTAAAATTTACCAGGAAATTTGCAAC GGTTGGAAAATTTAGAAAGATTTATGATGTAGAAAAATACTTTTGAGATCTTTGTATGAGAGGAATTGAG TCAGTGAGGGAACACTGCTGGTTTCATTTTCGTAATCACCAGTGGGGCGTCTGATCATCCTGGTTGTTAT GTGATAGATGGCTCACATTGATTTGTGATTTTGAAACAAATAAAAAAAATTTACAAAAGAATATATAAGA GCAGGCAAGAAATTTAAATTACCAAGAGATGGGGGAAAATCTGTTCTTCCTAAAGAAATCCCTTCAGATA GAGCTCATGGTGTTTAGTGATGTACTTGCAGTATCGTTTGAAGAATTGTTTTGTCTTAAGGAAGATGAGA TGTTGCACACGTTTGGATGGCAGCAAGTCAGCAAAGGCGACCTGAGTTGGATATATTTGGAGGTATTTTG AGAGTTACGTTTAAGGCTAACACCTGAGCTTTGTGTGATGTAAATAAGACCTTGTGTTTATGAACCTTTC AGCTAATTTTATTTTTTTCCCTTACATATCAAGTGATGTTCAGGTTTTGAATGTTTTGTATCAGTTCTTT TCTTTGTAAATGGCATTAACATTGTTACTTGAGGTCTTGCTTATTCAGTTCTGTTGTCCTGAGGACTTGA ATTTACAGTGCATCAGATTCGTTGGCTAATTCTGTCTGTAGATAGTCTAGCTTCAGCTGTTTATGGTGAT GCTACATTTTCGTTTATAAATATGTTTGTGGTAAAAAAAAAAGAAAAAAATGAGTATAACCATAGGTTTT GAACAAATTTCCTTACATTTTTCATACAAAAATCATAAATATCTGTATGCTATTGAAATTTAACTTTGTA TGATGCTTAAACCACTATTTGGGGAAATAATAAAATAAGTCTTTACCATGTATGAAAGAAATTTTAAAAA ATACAAAATATTTTCTGATTAGCATCTAGCTTATAATAAATTTTCAAAAAAGCTGAAGGCAACTGTGGCC TTCATCAGGATGCACTGAGAACTATATAGTTACGTCCTGCGTTTTGTATAAACTGAGATGCTCATGTGCT TCCCCTTAGAACAGGCAATGTGCTATGCATAACATAGTTGTACATTATCTTTGCAGTTGCTTTGAGTTTT ATTTTTTATTATTTAAAACTGTAGTTATAAAAATTTTCAGTATAGTACAGTACATATACTGTGAGGCGCG TGCTAAAGTGAACAAGCGAGTTTTCATGCTGACCCACTCAATGCTATTCAGAACTCAATTGGCTTAGCAC TTTCTCATATCCTTAGGTGCATTTAGATTGTCAGAGTTAACCTGCGTTAAAAAAAAATAAAATAAAATAC ATGTATATATTTAAAAAAAAAAATAAGGTTTCCCTTGTGGGCAAACAGCAGCTACACGCTTCTTTCCTGT GCTACTGTAGCAAACCACAGCATTGATGAGAAGGCATGCGGACTGTGCTTCACTTCACAGTGTTTACCCG AGCACTTAATAAAATGTAAGGCTGGTATTTATTTGAAGTTGTACAGTATGACTTAATTCACATCTGTTGG AATAGAAAATATATTCTGTTGAGTATTTAAGAGGCTGTACATGTTTTCTTTTGTGTTTGGATTCTTTGTG CTTTTTCATGTTCGGTACATCAATAAACAAAGTTGAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312245 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312245 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17382 same embryo
	EMAGE:17380 same embryo
	EMAGE:17381 same embryo
	EMAGE:17378 same embryo
	EMAGE:17379 same embryo
	EurExpress:euxassay_003701 same experiment
	MGI:4824518 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source