EMAGE mouse gene expression spatial database

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EMAGE:17395

Tspan4 tetraspanin 4 ( MGI:1928097)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003838. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003838_01	euxassay_003838_02	euxassay_003838_03	euxassay_003838_04

euxassay_003838_05	euxassay_003838_06	euxassay_003838_07	euxassay_003838_08	euxassay_003838_09

euxassay_003838_10	euxassay_003838_11	euxassay_003838_12	euxassay_003838_13	euxassay_003838_14

euxassay_003838_15	euxassay_003838_16	euxassay_003838_17	euxassay_003838_18	euxassay_003838_19

euxassay_003838_20	euxassay_003838_21	euxassay_003838_22	euxassay_003838_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17395_wholemount_strong.wlz

17395_wholemount_moderate.wlz

17395_wholemount_weak.wlz

17395_wholemount_possible.wlz

17395_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17395_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20

viscerocranium

moderate

regional

Expression in the turbinate bone.

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 11 12

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 13 14 15 16 17 18 19 20

palatal shelf

moderate

regional

moderate expression: see section 09 10 11 12 13

upper jaw incisor

moderate

regional

moderate expression: see section 09 10

upper jaw molar

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17 18 19

bladder

moderate

regional

moderate expression: see section 10 11 12 13

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 14 15 16 17

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

sternum

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7786
Entity Detected:	Tspan4, tetraspanin 4 ( MGI:1928097)
Sequence:	sense strand is shown >T7786 CGCATCTCCCTGAATCCCGCCGCAGCCCTGCCCCGGCTCCCATGATTACCCTGAAGCGGCGGTGTTCCTT GCTCGTGCCCACCCGGGGCTCCGAGAGCCTCCGAGCCCGCGGCAGCATCCCGGTCCCCCCCCTCCCGCTG TGGTGGTAGATCCCGGCGCTACGGCGTCCGAGCAAGGCCGAAAGGTCCGCGGCCGGCGGTTCGCGTTTCT CGTGTTGCCTTGACTGACAGCTGCTCCGCCGTGCGGCGGCGCGCACAGAGCTGAAGCGCTGCAGCATGGC GCGCGGCTGCCTCCAGGGCGTCAAGTACCTCATGTTCGCCTTCAACCTGCTCTTCTGGCTGGGTGGCTGT GGTGTCCTGGGTGTTGGCATCTGGTTGGCTGCCACACAGGGAAACTTTGCCACCTTATCATCCTCATTTC CATCCTTGTCGGCTGCCAACCTGCTCATCGTCACCGGGACCTTCGTCATGGCCATCGGCTTCGTGGGCTG CATTGGGGCCCTCAAGGAGAACAAGTGCCTACTGCTCACTTTCTTTGTGCTGCTGCTGCTAGTGTTCCTG CTGGAAGCCACCATTGCTGTGCTCTTCTTTGCCTACAGTGACAAGATTGACAGTTATGCCCAACAAGACC TGAAGAAGGGCCTGCATCTGTATGGCACACAGGGCAACGTGGGCCTCACCAATGCCTGGAGCATCATCCA GACTGATTTCCGATGCTGTGGAGTTTCCAATTACACTGATTGGTTTGAGGTATACAATGCCACTCGTGTG CCTGACTCCTGCTGTCTGGAGTTCAGTGATAGCTGTGGGTTACATGAACCTGGTACCTGGTGGAAGTCGC CCTGTTATGAGACAGTGAAGGCCTGGCTCCAGGAGAACCTGCTAGCTGTGGGCATCTTTGGACTGTGCAC GGCACTGGTGCAGATTCTGGGCCTCACCTTCGCTATGACCATGTACTGCCAGGTGGTAAAGGCGGACACC TACTGTGCATAGGTCTCACAGCCTCTGCTTCTCTGCCAAAGGACCCGCCCACGGGAAGGAAGATGTCCAA ACCCGCTCCCCCTGCAGGACTGGTGTTCTGCTGAATGCAGGGGGAAGAGCTGGAGGCCAGAGCACCCAGC ATCCATCCCTGCACCTGAGGGCACTCTGCTTGGGTGGCAGCAGACACCAGACTATAGAGTTGGTGCCAAA TGGCACCCAGGGCTAAGGGCCCAGGGAACTTTGACACTTTTTATATCCATATATACCTTCCAGTGTTCAC ACAGGATGGGGTTCCCAGCCTTGCATGGTTGCTAGGTTGGGGGCAGGCCCAGTCTGCTCAAGGCCCACGA TGATCTTGGTGCTCCAGGCAGGGCCAGGTCCCATGATCCACATTCCATGACAGTGTCGTGGTTCCAGATG CATCTTAATAAAGTGTGTGAGCAGACTGTGGGTCTTGCCAACACACAAAGGTTGTACAGCCAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314296 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314296 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17394 same embryo
	EMAGE:17399 same embryo
	EMAGE:17398 same embryo
	EMAGE:17397 same embryo
	EMAGE:17396 same embryo
	EurExpress:euxassay_003838 same experiment
	MGI:4828968 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source