EMAGE mouse gene expression spatial database

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EMAGE:17438

Bglap-rs1 bone gamma-carboxyglutamate protein, related sequence 1 ( MGI:88155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003880. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003880_01	euxassay_003880_02	euxassay_003880_03	euxassay_003880_04

euxassay_003880_05	euxassay_003880_06	euxassay_003880_07	euxassay_003880_08	euxassay_003880_09

euxassay_003880_10	euxassay_003880_11	euxassay_003880_12	euxassay_003880_13	euxassay_003880_14

euxassay_003880_15	euxassay_003880_16	euxassay_003880_17	euxassay_003880_18	euxassay_003880_19

euxassay_003880_20	euxassay_003880_21	euxassay_003880_22	euxassay_003880_23	euxassay_003880_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17438_wholemount_strong.wlz

17438_wholemount_moderate.wlz

17438_wholemount_weak.wlz

17438_wholemount_possible.wlz

17438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 16 17 18 19 20

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13

submandibular gland primordium

moderate

graded

moderate expression: see section 06 07 08 09 17 18 weak expression: see section 15 16

thyroid gland

moderate

regional

moderate expression: see section 09 10 14

adenohypophysis

moderate

regional

moderate expression: see section 12 13 14 15

vibrissa

moderate

regional

moderate expression: see section 05 06 07 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 15 16 17 18 weak expression: see section 09 10 11 12

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 15 16

trachea

moderate

regional

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7282
Entity Detected:	Bglap-rs1, bone gamma-carboxyglutamate protein, related sequence 1 ( MGI:88155)
Sequence:	sense strand is shown >T7282 CGGACGCGTGGGTCGACCCACGCGTCCGGACCCACGCGTCCGGAGACTTATTCTTGGAGAGAGACTCCTG CATGCATGTTGAAAGGTTCCTGAATAAACTGCTTTGAGAAGAACATAGTGTCGTCGTTTCTTTCTGCTGG TCAGAATCAGAGGCAACAGGAGGGTGCAGAACAGACAAGTCCCACACAGCAGCTTGGTGCACACCTAGCA GACACCATGAGGACCCTCTCTCTGCTCACTCTGCTGGCCCTGGCTGCGCTCTGTCTCTCTGACCTCACAG ATGCCACGCCCAGCGGCCCTGAGTCTGACAAAGCCTTCATGTCCAAGCAGGAGGGCAATAAGGTAGTGAA CAGACTCCGGCGCTACCTTGGAGCTTCAGTCCCCAGCCCAGATCCCCTGGAGCCCACCCGGGAGCTATGT GAGCTTGACCCTGCTTGTGACGAGCTATCAAACCAGTATGGCTTAAAGACCGCCTACAGACGCATCTACG GTATCACTATTTAGGACCTGTGCTGCCCTAAAGCCAAACTCTGGCAGCTCGGCTTTGGCTGCTCTCCAGA ACTTGATCCTCCCTGTCCTCTCTCTCTGCCCTGCAAGTATGGATGTCACAGCAGCTCCAAAATAAAGTTC AGATGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4166503 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4166503 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17440 same embryo
	EMAGE:17442 same embryo
	EMAGE:17439 same embryo
	EMAGE:17443 same embryo
	EMAGE:17441 same embryo
	EurExpress:euxassay_003880 same experiment
	MGI:4823464 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source