EMAGE mouse gene expression spatial database

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EMAGE:17451

Tspan13 tetraspanin 13 ( MGI:1913359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003891. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003891_01	euxassay_003891_02	euxassay_003891_03	euxassay_003891_04

euxassay_003891_05	euxassay_003891_06	euxassay_003891_07	euxassay_003891_08	euxassay_003891_09

euxassay_003891_10	euxassay_003891_11	euxassay_003891_12	euxassay_003891_13	euxassay_003891_14

euxassay_003891_15	euxassay_003891_16	euxassay_003891_17	euxassay_003891_18	euxassay_003891_19

euxassay_003891_20	euxassay_003891_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17451_wholemount_strong.wlz

17451_wholemount_moderate.wlz

17451_wholemount_weak.wlz

17451_wholemount_possible.wlz

17451_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17451_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

physiological umbilical hernia

moderate

regional

moderate expression: see section 11

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 16 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 06 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 14 15 16 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 12

cervical ganglion

moderate

regional

moderate expression: see section 07 13

thoracic ganglion

moderate

regional

moderate expression: see section 08 09

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 11 12 13 14 weak expression: see section 10

neural retina

moderate

homogeneous

moderate expression: see section 01 02 03 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 12 13 14 15 16 weak expression: see section 07

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

rectum

moderate

regional

moderate expression: see section 09

bladder

strong

regional

Expression in the fundus region.

male reproductive system

moderate

regional

moderate expression: see section 09

urethra of male

moderate

regional

moderate expression: see section 09 10

left lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

right lung

moderate

regional

moderate expression: see section 11 12 13 15 16 17 18 19 weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7280
Entity Detected:	Tspan13, tetraspanin 13 ( MGI:1913359)
Sequence:	sense strand is shown >T7280 GGGAGCCGCTGCGGGCGCGGCACTGCAGCCCTGATCCTGAACCCCGAACCCGAGCCCCGAGCCTAGCCCG GGCCACAGGCTCAGGAGGCCAGGATCCAGAAGCTACTCGCCCAGCCCGGCGGAAGACGCCTCGACCCGCA GGCACCCGCTAGCCCACGCGCCACGATGGTGTGCGGGGGCTTCTCGTGCTCCAAGAACTGCCTGTGCGCG CTCAACCTGCTGTATACCCTGGTCAGCCTGCTGCTCATTGGGATTGCTGCCTGGGGCATCGGCTTTGGGC TGATCTCCAGTCTCCGAGTGGTGGGTGTGGTCATTGCTGTGGGCATCTTCCTGTTCCTGATTGCCTTGGT GGGGCTAATTGGAGCTGTAAAACACCACCAGGTGTTGCTATTTTTTTATATGATCATTCTCCTACTTGTA TTTATTGTCCAGTTTTCAGTATCTTGTGCTTGTTTAGCTCTGAATCGGGAGCAGCAGGGTCAGCTGCTGG AAGTCGGATGGAACAATACAGCAAGTGCTCGAAATGATATCCAGAGGAATTTGAACTGCTGTGGCTTCCG GAGCTATAACCCAAATGACACCTGTCCAGCTAGCTGTGCTAAAAGTACCCAGAAGTGCTCCTCGTGCGCC CCCATAATCGGAGAGTATGCTGGAGAGGTTTTGAGGTTTGTTGGTGGCATTGGTCTCTTCTTCAGTTTTA CAGAGATCCTGGGTGTTTGGCTGACCTACAGATACCGGAACCAGAAAGACCCTCGCGCCAACCCGAGTGC TTTCCTTTGACGAGAAGACAAAGAAGACCTTTCCTCTGTTGATCTTGTTTTCTTTCTCTAATCTCAAATT AAACTAATTTGTTCATACGACAAAGGAAGCAGTATCTGAAAACCACATTATGTCAACGATGGAATTAAAA ATTTTACTCTTAAGTTTCTCCAGACATTTTTTTTTCTTTCCATTGCTAAAAAAGCAATCGAAGTTCCCCA TATTCCCTGGCTCTCAGTGGTCCCTGTAATCTACTGTTGTTGGTGTCTGGCACTGTCCACCGTGGCCTTT CTTAGCATTTTTACCTGCTGTAAACTGTGTATGGCACCCCTGGGTAGATGATATTTGACCCCAGATCTTC ATCTCACTGTCTAATTACCCAGAGCACTGGGGCTCCCACCCAAGACAGTTGATGTTTTATGAATCCAGAA AGGAAGAGACTCTGCTTTGTTCAGTTCATTAGGAGAAATCTACGTTCCTGATACTTTTTGCCTTTTGGAA AAGCTACATTGTAGTGAAAAGTGTTAGCACAGAAGTGATAATTTAGTTCTAGTAGTCTGTCAGGATTGCA TCCCTGTGTTCTAGAAATAGCTGTGTCTTTAGGAAAATGAAATTTAGTTTTTGACCTTTTTAGTCAAGGG CAAAGGAAGATACCATTTGGGAAATGCTTTGGTGTGTAACATCTTCAGCTCTCAAAATGGGATGAATTTG AGGAAGATAGAGCATATATCTACATAATCTGATGTAAAAAAAAAAAATACGAAGTTCAAAACACTGTATA TGTAAACAACTTGCCGTCAATACATTGGCCCAGGCCCATGTAACAAATAGATATTTGGTTATCTTTAAAA AATAACTTTTTTTTTTTCAAGACAGGGTTTCTTTGTGTAGCCTTGGTTGTCCTAGAACTAGCTCTGTGGA CCAGGCTGGCCTTGAACTCACAGGGATCCGCCTGCCTCTGCCTCCCAATTGCTGGGATTAAAGGCACGTG CCACCATCGCCTGTCAAAAAATAACTTTTTAATGACATTTCTTGGTATTGTGACAGCAAGTTGTCAAATC TAAATATATTTGAATGTACTACCTCCCATAAGTTGAGAGAGAAATGGTGTTTGTGGCTCTGTATATTACA TTAAAAAATAAAAGCTGAAAACCTTCCTTTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4166625 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4166625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17450 same embryo
	EMAGE:17449 same embryo
	EMAGE:17452 same embryo
	EurExpress:euxassay_003891 same experiment
	MGI:4828959 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source