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EMAGE:17464

Fbln7 fibulin 7 ( MGI:1917620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464
"Pseudo-wholemount" of euxassay_003912. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003912_01 euxassay_003912_02 euxassay_003912_03 euxassay_003912_04
EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464
euxassay_003912_05 euxassay_003912_06 euxassay_003912_07 euxassay_003912_08 euxassay_003912_09
EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464
euxassay_003912_10 euxassay_003912_11 euxassay_003912_12 euxassay_003912_13 euxassay_003912_14
EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464 EMAGE:17464
euxassay_003912_15 euxassay_003912_16 euxassay_003912_17 euxassay_003912_18 euxassay_003912_19
EMAGE:17464 EMAGE:17464 EMAGE:17464
euxassay_003912_20 euxassay_003912_21 euxassay_003912_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17464Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17464_wholemount_strong.wlz
17464_wholemount_moderate.wlz
17464_wholemount_weak.wlz
17464_wholemount_possible.wlz
17464_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17464_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 12 13 14 15 16 17 18 19 20
humerus
moderate moderate
regionalmoderate expression: see section 17 18 19 20 21 22
hand
moderate moderate
regionalmoderate expression: see section 01 02 04 19 20 21 not examined expression: see section 03
foot
moderate moderate
regionalmoderate expression: see section 01 02 04 05 06 07 08 09 10 19 20 21 not examined expression: see section 03
not examined not examined
regionalnot examined expression: see section 04
not examined not examined
regionalnot examined expression: see section 04
femur
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 15 16 18 19 20
thymus primordium
moderate moderate
regionalmoderate expression: see section 08
otic capsule
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 13 14 15 16 17 18 19 20
nasal septum
strong strong
regionalstrong expression: see section 13 14
viscerocranium
strong strong
regionalExpression in the turbinate bone.
pharynx
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13
hyoid
moderate moderate
regionalmoderate expression: see section 05 06 07 13 14 15
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19
palatal shelf
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
trachea
moderate moderate
regionalmoderate expression: see section 09
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14
basioccipital bone
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20
orbito-sphenoid
strong strong
regionalstrong expression: see section 04 05 06 07 16 17 18 19 20
scapula
moderate moderate
regionalmoderate expression: see section 17
sternum
moderate moderate
regionalmoderate expression: see section 10 11
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 06 07 21 22
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7297
Entity Detected:Fbln7, fibulin 7 ( MGI:1917620)
Sequence:sense strand is shown

>T7297
TGCAAGGGACCCGCGCACTGCGGCCAGAGAACAGCGACCTGCCTTCCCCAGCAGCAAGGCTGTGAGTGGC
AAGATGGGACCGGGCTCACAGCGCGCGCTTTTCCTCCTCTTGCTGCTCCTCGCCAGCCCCGGAGCGCGGG
CTTTCCAGAGCTGTCTCAACAAACAGCAGCTCCTCACCACCATCCGCCAGCTGCAGCAGCTGCTGAAAGG
CCAGGAGACCCGCTTCACCGAAGGCATCCGAAACATGAAGAGCCGGCTGGCTGCACTGCAAAACACGGTT
AACAAAATGACCCCGGATGCACCACCAGTTTCCTGCCCAGCTCTGGAGGCCCCTCCGGATGGCAAGAAGT
TTGGAAGCAAGTACTTAGTGGACCATGAAGTCTATTTTACCTGCAACCCTGGGTTCCAGCTGGTTGGGCC
CAGCAGCGTGGTGTGTCTTGCTAATGGTAGCTGGACAGGAGAGCAGCCCCGCTGCAGAGATATCAGTGAA
TGCTCCAGCCAGCCTTGTCACAATGGAGGGACGTGTGTGGAAGGCATCAACCACTACAGATGCATCTGTC
CTCCAGGAAAAACTGGGAACCGCTGTCAGCATCAGACCCAGGCTGCGGCCCCAGATGGCGGCGAGGCCGG
TGACCCCGCCTTCAGCCGCGCACCCCGCTGCGCGCAGGTGGAGCGGGAACAGCATTGCAGCTGCGAGGCG
GGATTCCACCTGAGCAGCACCACGGGCGGCCACAGCGTCTGCCAGGATGTGAATGAGTGTGAGATCTATG
GGCAGAAAGGACGCCCCCGGCTCTGCATGCATGCCTGTGTGAACACCCCTGGTTCCTACCGATGTACCTG
CCCGAGTGGATACCGGATCCTGGCTGATGGGAAGAGCTGTGAGGATGTTGATGAGTGTGCAGGCCCACAG
CACATGTGCCCCCGGGGGACCACATGCATCAACACTGGAGGAGGCTTCCAGTGTGTCAACCCTGAGTGTC
CTGAGGGCAGCGGCAATATAAGCTACGTGAAGACATCTCCCTTTCAGTGCGAGCGAAACCCTTGTCCCAT
GGACAGCAGGCCATGCCGCCACCTGCCCAAGACCATCTCCTTCCATTACCTCTCTCTCCCTTCCAAGTTG
AAGACACCCATCACGCTCTTCCGCATGGCCACAGCCTCAATTCCTGGCCATCCTGGGCCCAACAGCCTGC
GCTTTGGGATCGTGGGTGGGAACAGCCGTGGCCACTTCGTAATGCAGCGCTCAGACCGGCAGACAGGAGA
GCTCATCCTTACACAGACCCTGGAGGGGCCTCAGACTCTGGAGGTTGACGTAGACATGTCGGAATACCTG
GAGCGCTCCTTCCAGGCCAACCATGTATCCAAGGTCACCATCTTTGTTTCTCGCTATGACTTCTGAGGAT
GCCATGGCAGTCGGGAGGCTGGGGTTTGAGATCTGGGCTGATTTCACTTCCCCAAGGACACATTGTGGGC
AAGAGCTGTGGTGGTCATTCTTTTCTTGGTCATGTTCTTACCTACTCTGCTTGTTCATTCAGGCTTCTAG
ATCAATGCTGTGTTCTGACCCAGGGGCTGCCACAGAAGGGAAGCCACAAACAAATGCTGTTTCTACCATC
AAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4167912 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4167912 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17463 same embryo
 EMAGE:17462 same embryo
 EurExpress:euxassay_003912 same experiment
 MGI:4824791 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS