Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17520

Pebp4 phosphatidylethanolamine binding protein 4 ( MGI:1920773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520
"Pseudo-wholemount" of euxassay_005860. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005860_01 euxassay_005860_02 euxassay_005860_03 euxassay_005860_04
EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520
euxassay_005860_05 euxassay_005860_06 euxassay_005860_07 euxassay_005860_08 euxassay_005860_09
EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520
euxassay_005860_10 euxassay_005860_11 euxassay_005860_12 euxassay_005860_13 euxassay_005860_14
EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520
euxassay_005860_15 euxassay_005860_16 euxassay_005860_17 euxassay_005860_18 euxassay_005860_19
EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520 EMAGE:17520
euxassay_005860_20 euxassay_005860_21 euxassay_005860_22 euxassay_005860_23 euxassay_005860_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17520Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17520_wholemount_strong.wlz
17520_wholemount_moderate.wlz
17520_wholemount_weak.wlz
17520_wholemount_possible.wlz
17520_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17520_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 17 18 19 20 21 22 23
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 21 22 23 24
hindlimb digit 2 metatarsal
weak weak
regionalweak expression: see section 04
hindlimb digit 3 metatarsal
weak weak
regionalweak expression: see section 04
hindlimb digit 4 metatarsal
weak weak
regionalweak expression: see section 04
fibula
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 24
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 23 weak expression: see section 24
femur
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 23 weak expression: see section 24
not examined not examined
regionalnot examined expression: see section 21 22 23
mandible
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
basioccipital bone
weak weak
regionalweak expression: see section 01 02 19 20
basisphenoid bone
weak weak
regionalweak expression: see section 01 02 03 23 24
temporal bone
weak weak
regionalweak expression: see section 01
temporal bone
weak weak
regionalweak expression: see section 02 03 17 18 19 20 21 22 23 24
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 03 05 06 07 18 19 20 21
viscerocranium
weak weak
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9766
Entity Detected:Pebp4, phosphatidylethanolamine binding protein 4 ( MGI:1920773)
Sequence:sense strand is shown

>T9766
ATTCTCTAAGAATTATATAGGTCCTCAATAGCAGTTAACCTTCATCCCATTAGACAAATACTGTGAGGGT
ACCCCGAAGGGACCCCTGCCAGCTTCAAAACGGATCAAGATTCCCCAGGAGAGAACAACGGTAGAGAATC
TAATGTAAACCTGTTGCCAAGAACTATGACAATGAAGCTGGTCGCAGCAGCACTTTGCTTGAGCCTCCTT
GCAGCCGGCCTGTGGGTGGGCCTCTCATTGACAGCTGAGTCCATTGAAGAAGGGAAACCTGGAGGAGAGA
AACCTGGAGGAGGGAAACCTGGAGGCAGCGGACGAGGATGTTTTCTCCCACCACTGCCAAAAGAAGATGT
TTCACTATGCAGGAACCTAGAAGTTTTCTACATGGAGATGGGGAACATCAGCTGTAAAATTGTTCCTAAG
TGCAATCTATACAGACAGAAGATCACGGCCTGGCAGGCGCCGATAGTCAAGTTCCACACGGCTTTGGATG
TGAGTGAACTGGGTTGGCTGAAGGAGAACGTGGGGCCCTGACTTGCCTTTAGTTCACTTTTCTTGGGAAC
TTGGAGGGCCAGGTTTGCAGGAGTCAGGCAGCTATTGCCTGGAGTGGTGGTCAACCTCCCTAATCCTAAT
CCTTTATATAGTTCCTCATGTGGTGGTGACTCCCAACCATAAAATTACTTTTTGTTGCTACTTCAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6742909 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6742909 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17519 same embryo
 EMAGE:17518 same embryo
 EMAGE:17521 same embryo
 EMAGE:17522 same embryo
 EurExpress:euxassay_005860 same experiment
 MGI:4827149 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS