EMAGE mouse gene expression spatial database

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EMAGE:17522

1700029P11Rik RIKEN cDNA 1700029P11 gene ( MGI:1913596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005859. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005859_01	euxassay_005859_02	euxassay_005859_03	euxassay_005859_04

euxassay_005859_05	euxassay_005859_06	euxassay_005859_07	euxassay_005859_08	euxassay_005859_09

euxassay_005859_10	euxassay_005859_11	euxassay_005859_12	euxassay_005859_13	euxassay_005859_14

euxassay_005859_15	euxassay_005859_16	euxassay_005859_17	euxassay_005859_18	euxassay_005859_19

euxassay_005859_20	euxassay_005859_21	euxassay_005859_22	euxassay_005859_23	euxassay_005859_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17522_wholemount_strong.wlz

17522_wholemount_moderate.wlz

17522_wholemount_weak.wlz

17522_wholemount_possible.wlz

17522_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17522_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 15 16 17 18 19 20 21 22

humerus

weak

regional

weak expression: see section 01 02 20 21 22 23 24

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 04 22

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 04 22

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 04 22

fibula

moderate

regional

moderate expression: see section 01 weak expression: see section 24

tibia

moderate

regional

moderate expression: see section 01 02 weak expression: see section 24

femur

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 17 18 19 20 21 22 23

otic capsule

weak

regional

weak expression: see section 01 02 19 20 21 22 23

orbito-sphenoid

weak

regional

weak expression: see section 07 18 19 20

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 02 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9793
Entity Detected:	1700029P11Rik, RIKEN cDNA 1700029P11 gene ( MGI:1913596)
Sequence:	sense strand is shown >T9793 GCAGCTGTCATGGCGGCCTGGCGGTTAAGTTTGTGCGCTCGCAGCCTTTCCGCAATTCGACGGCTCCAGG CTGCCCCACTTTGCTGGAAATCCAGCTCCTCCAGGGCTGTGGTCGCCCGGTCCGCTGCGGCCCGAAAGGA GCAGGAAGAACCGACCGTGCAGTGGATGGAGGACCCAGATCCCGAGGACGAAAACGTGTACTTGAAGAAC CCACACTTCCATGGTTACGACGACGACCCGAAGGTGGATGCCTTGAATATGCACGCCGTCTTCTTCTTTG GTTTTTCCATCGCCCTGGTCCTTGGCACTACCTTTGTGGCTTATGTGCCAAACTATAAGATGATCGAGTG GGCCCGCCGGGAAGCTGCGATGCTGGTGAAACACCGAGAGGCCAGTGGCCTCCCCATCATGGAGTCCAAC TATTTTGACCCCACCAAGATCAACCTGCTGGAGGACGACTGAGTGGGGCTTAAGCAGCGCTACCCTGCTC ACTCCCCAGAGAATCTACCCAAGGGATTGAAAGAAGAAAAGAGTGTTACTCCAGCTAGGCCTGTCAATTT TTTTGGCCAAGGGCCCACACCTCAGTAACACTGATTGACCTAAATATGCTAAAGAGATGGGAGGAACAAT TATCTCCTTAATGAGAGAATGTATTTTCCTTGGCCGAAACTCTGTGGGCTCTAGTCACCTGTGGGGCCTT CACCTTGTGGTCTGCTTACTTCCTGGAGTTCTAAGTTCCAAAACGCATCTGTTTCTTTTCCCCATCTTTT GAATACTTGCCAATGTCTGGGCTCCCTCCCCCCACCATTGATGTACTGGGCTCTCTTGCTTTATTTGTAT CCCCCTCTTCTGCTTCTGCCATGGGGCTGCTTGGGAGCCTCCATTACTAAACTTCATATGTAACCCAAGA AGGCGTGGCTTTCTCCGTATTTCTTCTGGAATCGGCCTGGATTTTCAGATTGCCTGCCCCGCTATGTTTC TTTTAAACTATAATAAAATGGTTCTTAAGCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6770795 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6770795 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17519 same embryo
	EMAGE:17518 same embryo
	EMAGE:17521 same embryo
	EMAGE:17520 same embryo
	EurExpress:euxassay_005859 same experiment
	MGI:4822629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source