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EMAGE:17606

Papss2 3'-phosphoadenosine 5'-phosphosulfate synthase 2 ( MGI:1330223)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606
"Pseudo-wholemount" of euxassay_005940. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005940_01 euxassay_005940_02 euxassay_005940_03 euxassay_005940_04
EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606
euxassay_005940_05 euxassay_005940_06 euxassay_005940_07 euxassay_005940_08 euxassay_005940_09
EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606
euxassay_005940_10 euxassay_005940_11 euxassay_005940_12 euxassay_005940_13 euxassay_005940_14
EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606
euxassay_005940_15 euxassay_005940_16 euxassay_005940_17 euxassay_005940_18 euxassay_005940_19
EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606 EMAGE:17606
euxassay_005940_20 euxassay_005940_21 euxassay_005940_22 euxassay_005940_23 euxassay_005940_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17606Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17606_wholemount_strong.wlz
17606_wholemount_moderate.wlz
17606_wholemount_weak.wlz
17606_wholemount_possible.wlz
17606_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17606_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24
humerus
strong strong
regionalstrong expression: see section 04 05 20 21 22 23 24
hand
strong strong
regionalstrong expression: see section 23 24
forelimb digit 1
strong strong
regionalstrong expression: see section 01 02 03 04 05
forelimb digit 2
strong strong
regionalstrong expression: see section 01 02 03 04 05
forelimb digit 3
strong strong
regionalstrong expression: see section 01 02 03 04 05
forelimb digit 4
strong strong
regionalstrong expression: see section 01 02 03 04 05
forelimb digit 5
strong strong
regionalstrong expression: see section 01 02 03 04 05
foot
strong strong
regionalstrong expression: see section 05
hindlimb digit 1
strong strong
regionalstrong expression: see section 06 07 08 09
hindlimb digit 2
strong strong
regionalstrong expression: see section 06 07 08 09
hindlimb digit 3
strong strong
regionalstrong expression: see section 06 07 08 09
hindlimb digit 4
strong strong
regionalstrong expression: see section 06 07 08 09
hindlimb digit 5
strong strong
regionalstrong expression: see section 06 07 08 09
fibula
strong strong
regionalstrong expression: see section 01 02 03 04
tibia
strong strong
regionalstrong expression: see section 01 02 03 04
femur
strong strong
regionalstrong expression: see section 04 07 08 09 18 19 20 21 22 23 24
naris
strong strong
regionalstrong expression: see section 12 13 14 15 16 17
nasal septum
moderate moderate
regionalmoderate expression: see section 12 14 15
viscerocranium
strong strong
regionalExpression in the turbinate bone.
heart valve
strong strong
regionalstrong expression: see section 11 12 13 14
hyoid
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
meckel's cartilage
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16
axial skeleton
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 19 20 21 23 24 not examined expression: see section 09 10 11 12 13 14
basisphenoid bone
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 22 23 24 not examined expression: see section 01 02 03 04 05 06 07 08 19 20 21
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23
vault of skull
strong strong
regionalstrong expression: see section 01 02
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 18 19 20 21
clavicle
strong strong
regionalstrong expression: see section 09 16
scapula
strong strong
regionalstrong expression: see section 20
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 10 11 16 17 18
axial skeleton tail region
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10059
Entity Detected:Papss2, 3'-phosphoadenosine 5'-phosphosulfate synthase 2 ( MGI:1330223)
Sequence:sense strand is shown

>T10059
ATTCCCATTCACACCCACCTCACAGACCTTCACAGACTCTGCAGCTCATTCATTCACCCCAATGGCCAGC
AAAGCCAGTTTGTAACCGAGTATTCTCAACATCAGATATCATGTCTTGGAGGAAGTTACCTAAACTCTGA
AGAATTATCATGTCTGCAAATTTCAAAATGAACCATAAAAGAGACCAGCAAAAATCCACCAATGTGGTCT
ACCAGGCCCATCATGTGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATG
TACCGTGTGGCTAACAGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTT
GTATCTCACGCCATCCCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGG
GATTCTCTGCGGGGGACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGC
CGGCCTGGTTTGCATCACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCAC
GAATCAGCAGGACTCCCATTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACG
TAAAAGGACTCTACAAACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGA
GAAACCTGAAACTCCAGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTG
GTGGAACTTTTGCAGGAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGC
CAGAAAACAAAGTCGATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGA
TCTGCAGTGGGTGCAGATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAG
TACTTGCAAACTCTACACTTCGACACTCTACTGGACGGCGTGGTTCCCCGTGATGGAGTCATCAACATGA
GTATTCCCATTGTATTGCCCGTTTCTGCGGATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTT
GATGTACGAAGGTCGGAGGGTCGCTCTATTACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGC
TGTTCTCGTGTGTGGGGAACAGCCACTGCAAAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACT
GGCTTGTTGGTGGAGACCTACAGGTGCTAGAGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCT
TACGCCTCTGGAGCTCAAACAGAAGTGTAAAGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGC
AATCCTGTCCACAATGGTCATGCCCTCCTGATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACA
AGCACCCAGTCCTCCTGCTCCACCCTCTTGGGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAG
GATGAAACAGCATGCAGCTGTACTGGAGGAAAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTT
CCATCTCCTATGTTATATGCTGGTCCCACAGAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAG
CCAATTTCTACATTGTGGGTAGGGATCCCGCAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGA
ACCCACCCACGGGGGCAAGGTCTTGAGTATGGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGA
GTGGCTGCCTACAATAAAATTAAAAAGGCCATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACT
TCATCTCAGGAACTCGCATGAGGAAGCTCGCCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCC
GAAAGCGTGGAAAGTGTTGACAGATTACTACAGGTCTCTGGAGAAGACCAACTAGGTGCTCCTGGCTCTG
GCTTCTTCCTCAAGTGCTCTCTGACGATTTTTTTTTTCTATTTTTGTGATTTAGCTGCTCTGTATCCAAT
TGCTTCTCGGTGACTTTTTAAAGCTAGTATTTTTGCAATGAAGTAAAAAGCTGTAACCATAATTTAAAAC
CAAGTTCATTATGTCTATGAAGCTCACACTGGAGACTGAGTCTTAGGTGAAAGCAATATCGTTGTACGTT
CCAGAATGAAAGCATTTGCACTGTACACTTCATCTCAGCCACTATGTCTCAGATTTCTTAAAAATGTAAC
TGTGTGGCTAGCTGACTAGCCCAGAACAGAATCATACTTTCCAGCTTACTTGTGCCAGCCTGGAATCTCA
ATGTCACTTACCAAAAGAAAACACACACACACACACACACACACACATACATACACACACACACACACAC
ATATACATACACACACACTGTCGCTTTGAAGGAAATGTGTTATATCAGGACTCTTACTTCATAACTACAC
ATACCATCACTGCCCAATGTACGAGCTGAAGATGCAACTGGAAGAAAAATTCATGTGAAGATGTAAATTA
AGTCTTAAAGAACAATGTAATTTATGTCTGCCATGAAAATGTCATCATCCAATAGAGAGAGACGCTCTAA
TGGCCTTCTATGAATAGTTACTCTAGTTTGGCTGGCACTTTTCAAAAGCATGGTATGCTTGGATCAAAAC
AACCCCCCCCCCAGACTAAACCACCAGGGTATATTGTAGACTACTTTCCCCACACTTTGCTGTGGCCATA
GTTATTATAGAAGCACCTAAGTGATCGCTGTAATCAAAACCAACCCACATTATTAATGAGAAGCTAAAAC
TTGATTTCTGTAGCAAAGATAAAATCCAAACATCATCCTAGTGAAGATATATACACATAAGCTTTGAGAG
CTGCTGAGCACTGTAATATACAAAGTTGACATTAGTGAATACTGCCGACACTGAAAGACTGACCATCCTC
CTAAGCTAGGGCCCTGCCTACCCTAGACAACACAGGAAGAGGTTCCGATTTCCTGAAGCTACTTAGTGAA
GCTCTCCTTGAGGATGCTAAGAAGCAACCACCTTGGAGACCGAAGGTTTAGGGTATCCCAAACTCTTGAT
GTTATAAGTTTCCAGAATGAACCTGTTGTGAGATGTCCTTTGATGGTTTATTTGCTAAGAGGGAATTTTG
GTATTTTATGCATGGTTCATTGTTGCCAAGAAATACATAAGAGAAAAGGCATCTTTACATACAGGTTTCT
TTGGAAGAGACCACAGACTGAAAAGAGAAACTGATTTTTTTCATACTGCCACGTTTTTTAAAAATGCTAG
GAAAAGCTCTACTGAGAGTAAGTTATATACTGTGACCACAGCAAAGTGAGTCTTCGACTGTATGGCGCCT
GTGCTCACTTACATCAGAGCTATTTATTCACTGCTGCCTTTAAAACTATGTCTTCACTGAAAACGTGACA
GGGTTGTAGTGCCTTCTTAGCATATGAAGTTGTGATTTTAAGACAGTAGAGTGCCTTTCTGCTGTAGCAC
ACAGCATAACTGTTTCAGTGTCCCGCTGGTACCTGTTCTTATTCAATAAATCTCTCAAAAGACCAAAAAA
AAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30121331 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30121331 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17603 same embryo
 EMAGE:17605 same embryo
 EMAGE:17604 same embryo
 EurExpress:euxassay_005940 same experiment
 MGI:4827044 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS