EMAGE mouse gene expression spatial database

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EMAGE:17640

4930432K21Rik RIKEN cDNA 4930432K21 gene ( MGI:1921916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005969. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005969_01	euxassay_005969_02	euxassay_005969_03	euxassay_005969_04

euxassay_005969_05	euxassay_005969_06	euxassay_005969_07	euxassay_005969_08	euxassay_005969_09

euxassay_005969_10	euxassay_005969_11	euxassay_005969_12	euxassay_005969_13	euxassay_005969_14

euxassay_005969_15	euxassay_005969_16	euxassay_005969_17	euxassay_005969_18	euxassay_005969_19

euxassay_005969_20	euxassay_005969_21	euxassay_005969_22	euxassay_005969_23	euxassay_005969_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17640_wholemount_strong.wlz

17640_wholemount_moderate.wlz

17640_wholemount_weak.wlz

17640_wholemount_possible.wlz

17640_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17640_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 15 18 19 20 21 22 23 24

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19 20

vibrissa dermal component

strong

regional

strong expression: see section 05 06 07 08 09 10 20 21 22 23

diencephalon meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

choroid invagination

strong

regional

strong expression: see section 07 08 09 10 11 12 13 17 18 19 20 21

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

perioptic mesenchyme

strong

regional

strong expression: see section 04 05 06 21 22 23 24

anterior naris epithelium

strong

regional

strong expression: see section 12 13 14 16 17

external naris epithelium

strong

regional

strong expression: see section 13 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vomeronasal organ epithelium

strong

regional

strong expression: see section 13 15 16

kidney pelvis

strong

regional

strong expression: see section 08 09 17 18 19 20

ureter

strong

regional

strong expression: see section 10 11 12 15 16 17

renal cortex

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19 20 21

clavicle

strong

regional

strong expression: see section 07 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9537
Entity Detected:	4930432K21Rik, RIKEN cDNA 4930432K21 gene ( MGI:1921916)
Sequence:	sense strand is shown >T9537 GGTGTGGATGCTCCTGCTCTCCCGTCTGTGGCGCAGGTCTTCAGGAGCCGAGTCCACAGTGTCAGGCCTC AGGCCTTCCTGTCTCCCACCGCCAGTTTGCCACGCGGTCCCCGTGACTGCCTATGTTGGTATTTTCTCAG AACGTTACGCCAGTGACCTAGGCGACCTATTCCCCATCAGATCCAGGTTGACCTCGTCCCGACTAAGGAC AGAGCAACTTCCATTACTGCTGTGGCCCAGATTTCTCAGGGAAAGATAAAATGAATAAGAAGAAACAGCG GAACTCAGGGGTCGGCCTCCATCCCTCAAAGCCTTCTAAGAACCCACGGCTGAGAGACTCCCATAGCTCC ATGATGGTCCATTCACACTACTCCAGAGAGTCGGAAGACAGCTCAGAACCTGCTCCTTCTGTAGAGCTAG GCGGGGAGGAACCACTACACGAAGCTTTCAGCTGCCCAGTGGAGGACACAGGAGCTGCCTCTGACCTCGC AGGATCACCCAAAGAGCTGGTCCCCCTGCCTCCTTCCCAGAACTCAGTTGGGAAGTTTGTCCCTCAGTTT GCCAAACCAAGAAAGACAGTAACTAGAAAAGCCAAGGCATGGGAAGAGGATCTAGAGGGCTGCACCACAA GCCAGGAAACAAGGCCAGAGCTGGGAGCCCTCAAAGCAGCCAGCCAGCCACAGAGAGAGTCCCTGAGGTT CCCTCCCCATGACATCAGACCAGAAGTCCAGACACAGCCAGATGGAACCCTTTCAAAGGAGAGGACAATC TCACTAGATAATAGAAGCCTGGGGAACAATGGTTTTGAGATGGCCACAGTTCAAGACAGTAGTAGCCCCC TGTCAGATGCTGCTGCTGAAGGCAGGGAGGCAGACAGCAGAGACCCACAGGAAAGAGATGCCCAAGGAGG GGAAGCAGGGGCCCAGCATTCTGGGGAACCCCAGGAAGGAGAGGACATCCTCTACACATCAGCATTAGCT CCTGCCTCAGAGCCCACCTGGTCTGTGGCTCAAGACCTCTCTGTCCCCACATATACTCTCAGCAGCACAG CTGCAGCACCCAGCTCCACCAGCCCAGCAGATGCCTCTCTTATGGACACTGTCATTACAGAAGCGAGCTT AGATCCCTCTGTGCTGCAACAGAGTGCTCCACAGGTGGCCAAGCTGCTGGGCTCTCTGGATGAGCAGATC CCTGATGGAGGCTGCATTGGAACTCTGCTGAGCTCCACACCTCTTGCTGAGGAGACCACAGCAGGCAGAG AGGAGGCCAGATGGGAAGAGAGGTGTCATGGTGATACCCTGGCAAGCTTCACTGAGACAGAGCCTGAGAA ACAAGAGCCCGTGACGGAAGCTGGAGATTCCGGCCATATAGCCCAAGAAATGGATCCAGTGGTCAAGACC AAGGACTCTGGCTCTGATGAGCAGTCGCCAGGGGATATAGGAATGCTGCCTTTACCAGCACAGTCCATGA ATCAGATGTTGGTAGAGTTACGTGGCCTAACTTGTGATCAAGACTTAGAGGGCCTCAGTACCCCTCATAC CTCCTCTCAGCTAGAGCACACTTGTGCAGCTAGTGACCCTCCCCAGAGCACCAAGGACTGTCACAGCAGC CCAGGCATTCCTGTACACCTGGCAGCTCCCTGTCCCAGAGACCAGGCTGCATGGCAGGAGTCCTCAGCAA TGGAACTAGACTTCCTGCCTGACAGCCAGATACAGGATGCCCTGGATGCCACTAACATGGAGCAGGGTTT TCCTTCAGGGAGTATGCCAGACCTTGGCTGGCCTGTCCCTAGCTCACAGTCCATCGGAGGATCCCCAAAG GCAGTCACCAAACCTCAATCCAGATCCCATGTGGAAACCTGGGCCCAGGAGACCTACAGGATGCAGGATG CAACAGACACAGTGCGAGGCCTCGTGGTGGAGCTCTCCGGTCTGAACCGGCTGATTATGAGTACCCACCG GGACCTGGAGGCCTTCAAGCGCAGGAAGACCAAGTCTCTGCCTTACCTGACAAAGGGGTTGGGGAGCCTT CCTAGAGGGGATCAGAGCTGGAGGGACTTGTAATCTGCTGCAGCCTGTAGGGGTCGGCTTACCATGGCTC CAGATGCAGGAGGCTGAGGGTGGCCACTTGGCCAGGATCACTATTGCTCCCATGCAACAGGGTTGCCTGA GCTTTAAGGAAGTCACCTGACAGAGCATGGCCCTACTCCCGATGCTCATCCCGGTTTGAGCATGCAGGAG TCTGAGCTGAGCTGCAGTTGTCGTTGAGTTTGTTTCTGGAGGAGAGAGGTGCCCTCTGTCTGCCTATCCT TCCCCCATGCTCTCTGTTACATTTGGGAAGGTGGGGAATAAATGATGCAGTCATGAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30607069 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30607069 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17643 same embryo
	EMAGE:17642 same embryo
	EMAGE:17641 same embryo
	EMAGE:17639 same embryo
	EurExpress:euxassay_005969 same experiment
	MGI:4822723 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source