EMAGE mouse gene expression spatial database

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EMAGE:17652

8430427H17Rik RIKEN cDNA 8430427H17 gene ( MGI:1918765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006045. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006045_01	euxassay_006045_02	euxassay_006045_03	euxassay_006045_04

euxassay_006045_05	euxassay_006045_06	euxassay_006045_07	euxassay_006045_08	euxassay_006045_09

euxassay_006045_10	euxassay_006045_11	euxassay_006045_12	euxassay_006045_13	euxassay_006045_14

euxassay_006045_15	euxassay_006045_16	euxassay_006045_17	euxassay_006045_18	euxassay_006045_19

euxassay_006045_20	euxassay_006045_21	euxassay_006045_22	euxassay_006045_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17652_wholemount_strong.wlz

17652_wholemount_moderate.wlz

17652_wholemount_weak.wlz

17652_wholemount_possible.wlz

17652_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17652_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ear

moderate

spotted

moderate expression: see section 01 02 03 04 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 05 06 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9584
Entity Detected:	8430427H17Rik, RIKEN cDNA 8430427H17 gene ( MGI:1918765)
Sequence:	sense strand is shown >T9584 AGATTTCTTTGACATCATCTACTCGATGCACGTGGAGAGCTCTGCAGAGCCAGGCAAAGCCCCCAAGCAC GCTGGGCAGAAGAAAACCTACCGAGCGATCGCGGAGACCTACGCCTTCCTCCCCAGAGAGGCTGTGACTC GGTTCCTCATGAGCTGCACGGAATGCCAGAAGAGGATGCACTTTAACTCCAGCGGCCCGGAGCCCAAAGA GAATGAGCCTCCATCCCCTCTGGTGTCTGGCATTATTGATTACAACATGCCCCTCACCTCTACCTACTTG AAGCAGATGAAGCTGCGAGTGATGAACTCTCAGGAGCAGGATGAAACGTCTGTGAGCAGTGAAGACTTTG ATATGAATGACTCCGCATGGATGTCAGCTGACCCCCACCTGGCCTCCAGCCTTAGCCCCAGCCAAGAGGA GAGGATGCGGAGCCCACAGAACCTCCATAGCCAGGAGGATGATGACTCCTCCTCTGAGAGCGGCAGCGGC AATGGCTCTTCTACCCTGAACCCATCCACTTCAAGCAGCACACAGGGGGACCCCGCCTTCCCTGAGATGA ATGGCAACGGAGCTGCAGCCCCCATGGACTTCACCGCCACTGCCGAGGACCAGCCCATTAACCTGTGTGA GAAGCTCCCGCCCGGCACAGCTCTGGGCACGCCGTCCTACCCCTCAGACGGCTGCGGCACTGATGGGCTG CGGAGCCGCGTCAAGTATGGGGTAAAGAACACCCCCGAGTCACCCCCATACAGCTCCGGGAGCTATGATT CCATCAAGACTGAAGTCAGTGGCTGTCCTGAGGACCTGACAGTGGGCCGGGCCCCTGCAGCAGATGACGA TGACGATGACCATGATGACCATGAGGACAATGACAAGATGAATGACTCAGAGGGCATGGACCCCGAGCGG CTCAAGGCCTTCAACATGTTCGTGCGGCTCTTCGTGGATGAGAACCTGGACCGCATGGTGCCCATTTCCA AGCAGCCCAAGGAGAAGATCCAGGCCATCATCGAGTCCTGCAGCCGGCAATTCCCTGAGTTCCAGGAGCG GGCCCGCAAGCGCATCCGCACATACCTCAAGTCCTGCCGCCGCATGAAGAAGAACGGCATGGAGATGACC AGACCTACTCCTCCGCATCTGACCTCAGCCATGGCAGAGAACATCCTAGCAGCTGCCTGTGAGAGTGAGA CGAGAAAGGCAGCCAAGAGGATGCGCCTGGAGATCTACCAGTCCTCACAAGAAGAACCCATAGCCCTGGA TAAGCAGCACTCTCGGGACTCTACAGCCATCACCCACTCCACCTACTCACTGCCAGCCTCTGCCTACTCC CAGGACCCCGTGTATGTCAACGGTGGCCTCAATTACAGCTACCGTGGCTATGGGTCCTTGAGCAGCAACT TGCAGCCCTCTGCTTCCCTCCAGACAGGAAATCACAGTAATGGGCCCACAGACCTCAGCATGAAAGGCGG GGCCTCTACCCCAACTCCTCCCACGCCTACCCCTTCCAGCAACAGCACCAGCAGGACCATGCCCACAGCC CAGCTTAGCCCCACGGAGATCAGTGCTGTGCGGCAGCTCATCGCAGGCTATCGAGAGTCTGCAGCCTTCC TGCTGCGTTCTGCAGATGAACTGGAAAACCTCATTTTACAGCAGAACTGACCCTGCTGGCCCCCAGAGTG CACGACGCCAGGAGGCTGTCCCAGCCTGGACCCAGCTTCCTGCCTCACCAGTTGGTACACTGTTTTCTGA AAGAGGTGGGATCCAAAAGAGCTGTTTCTAGCTGCACTCCAAGCATCTGAGACTTTGGGCACAAGGACAC TTTTCTTTTCTTTTTTTTTTTTAATTTCACCACATGGGTGCTCTGACCTGCTTGGAAAGAGGCCAGTGGG GCAGCTCTAAAAGGGTTGAGGTTCCCCTGAAAAGGCACAGGGGCTACAGCTCTATTTAGAATCACCTTTG TGGACCACGATGTTAGAACTCCCCCCCCCCATAATTACCTTTCAAGTCTTAGGTGAGCAGAATTGCATAT TTATTGAGAAGAGCAAAAGTGGACCCTTTCTCTCCCCTTAGTAATTTATTTTTCTGAAATGGATTCTTTT GTGTTTGTACAGATTGCCAGTCTGTGTCTGTCTAATCAGAAGGATGGATCCCTGTACCTAACATTCCATA TCACTACACTGATGTGAGCTGGGGGCCAGCAGAGTTGGGGGCCAGCTCACTGTCCCGAGTGCCCAGCATA CCCTTGGGAAGGCCACCACACTCCACCACTAGGCTGTCTTGTTGCAGATCCCAGTGCTGCCACAGGGACA CTGCCAAACAGAGGGACCTCACCAACACTCACTACAAACAAAAGCAGCCACCCATCCCATCCGACAGAGT CCCAGTCACACGGTCACCTGTACTCTACCTCACACTCCAGCGTGGGCTTTTCCAGGACGTGCTCCAAGTG TGTTCTGAACGGCTGTCTCCCCCAACTCCCACACAATGTAGCTATCTTCTGGGAGGTAAATGAGACCCAG TGTCCAGGAGCCAGAGCCCAGAGGGGCTTGGCCCTTAAAACCAGCAGCTTGCCATAGCCTGGGCTCTTGG AGATGCTTGACAGGATAGTGTGTCTGGAGCTGGTCCAGGTCCCACACAGCCCCCAGGTAACCTGCTGAGG GGCCACTTCTCTTGGCCAGGCATCTGTGTGCCCAAGGGTGAATTATGGTTTCCACTCCTCACCTCACGAT GGGAGTAGAGGGATCAGATCGTTGGTGGAAGTGCAGAACTACTTGCATTCTCCTAAGATCAAACTTTCTT CTGGAAAGGCCTGTCCTGTAAGAAACCTAGTCCCTGGAGTGTGACAAGGGACAAGAAAAAGCTGACCTGG TTTCCTTCCTCCCTGACAGGCACTTCCTCTTGCTCTGTGCAATACCTACCAGTGCTGCTAAAACCAGGGA TTCACCCAGACCTCAGCAGGCCAGGGGGCCTCTCCCTCAACACTCCGTAGCCATGACAGCAGCACTCTGC TGCCTGCCCCTGCCCAGAGATGGCAGAAGCCCTGTTGCCTTTCTTCCCTCAGAGCTAGGAGGCCTCAAGG GAGCCAGGGCTGAGTGGACACTAAGCTAACCACAAAGAGCCACTCTTGGTCCCAGAGCGAGTGCCCCTTC CCAAACTGGTCTATGGCAGGGACTGCTTGTGGCAGCCTGACAGACTACCCTTCAAACAGGTAGGCAAGCA GTGCCAGTGCAGATACCCTACCTGTTCAGATTCAGGGCTGTAAGGCAGGGTTCCCCACCCTGCACACTTC CACACTGGGGTTAAATGTGGTCTTTCACATGGCCTACAATGCCTACCCAGCCACACCACCTCTGCCTTCA TGTGCCCGCTCACCAGGCCTCTGGACTGGGCCAGGACACCTGAGCCCCTTCATACAGCACAAACTCCAGT GCCCAGGCCTCCCCACAGCTGGCCAAACATGTGCCCAGTTCCCCACCTGCTCAGTAGGACTCCTGGATGT TTGAATTTGTGTAAATATTTATTTTTGTGTGTGAATACTACAAAGTAATCAGTAGATCTTTTTGCAAAAT GTTACTCCAGGCAATTTGTGAAAATGTTGAAAATTGGCAGAGACAATCGCCACCCTAGAATTTCTGTTAT CAATTGCTTACCGCATTCTGTCTGCTCCCAGAGGCAGTACCCAAGCTAGACACAAATGGCTTGCATTTTT GGAAGAAGATAGAAGAGACTAGGCAGGGGCTCCTCCCTCTATCCCCACAGGACACATCTGTAAGGACGAG CATAATCTATATCTAAGTGGTATTTTCTAACTGCAAGCAGTCTTGACCAGCCACTAGCAGCCTGCTCCTA GAGTACGGTGTGAGCATAGGTTCCATGTGTTTTGCTTTCAAGAGAAATAAAACCAATGCCAGTGCTGATG GTTTCCAGAGGGGCTGCAGGACAGTAGTAGATGCTGGCATGCTGACCTGGGCTTCTTCCCAGAGAAGTGG TCTCCAAAGCCTCAGAGCAGCCATACCAGGAGCAAGTCAGTGTGGACACAGGATTCCATTACCTAGTTAA GTCAGGGAACTTGTTCTGTTTTCCTTTTGTTTTAAAAGATGCAGCGTCAAGCCTCCCTTCCTGACTGTGG CCCGCCTTTTCCCAGGAGGAGCTTCCCAAAGCCTTGTCCTGCAGCCACTGCAGTGTGATGGAAGCCTCAG GCACACAGTAGTCATTGGTGATCTCAAGTCATAGTGAACATGCCAGTGCAGGATGCACCCCCCCTCACCC CCGAGTCCTTACAGGCAGATCCCTGCAGGCTATCAAGAGAAGTGGTGGATGATCATCTGTTAGACCAGGG ATAGTATACAGGACCCATCTGAAAGCATTTTTTTTTCCTCTCACCACTCAACAAATGGACAGTTTATTCC CAAGGACGGTGTTGGGGAGAGGCAGGCAGGGCCAGGAGGGTCTTGGAGGACTTTGAACAGGTGGAACCCA TCTGTCCCTCCCTATTTCTCAAGGGCCACAAATGACTCCACAGGTGGTTATAGGATTAACTACCAGTTCA TTCTCAAAATGCTGCTTTGAACTCAGAGGGTTGATACTTTTAATTTGTAATTTTTTGTAAAACTTTTTAC AAGATAGTAAAGTATTTCACCAGAATACCAGTTTCAATCCGTCCGTGGTCTGATTTTATATAATTTAGTG GTGCTTTAGAGACTTTGTTTTGTTGTTTCTGAGCGCAACAGCTCAATCCTCTCTTGCCTGTATCTACCTA AGACCAATGTGAACCTTTGTATTTTGCTCCTAATTTTGGACTCAGTGGAAGTGTACTGTTTACATGTACA GATGCCCCATCCCTGTGTTTTCTGCAAGACGCACGCTCGAGGAGGAAGATTGCACCTCACTAGCTCATCT GCTTAGCAAAGCCACAAAACAAAACCTCTCACTTTGTACCCGTTTCCACCTAAAAAACAAAAACCTTCAC CACCTGGAACTCAGATTTGCCAACACATTCTGCCTGGTAGATGCCCCATGCCTCTATCATGTGTTCCCTT AGGAATCAAGGCAGCACCTCCTGCCAGCTGGGTGGGGGATAGCACACAGTGGAGACATTAATGCAGGACT TTACTGCCCAGCTCTGGGAGGAAGTTCTTCTTTGGGCAGGAGGCAGTATGAAGGTCAACTCATGGGCCTT CAGGAGCAAGCCAGGCAGGATTTAGGGACAACACAAGAGGCCATCAATCTGCTGGCATGATTGAGACACA CTTTGGAAGAGCATGAGTTCCATGTAAGGTGAGACTGTGACTTTGAGTGCAGATGACACAGCACTTCAGG GGAGGGTCAGGGGCTCCAGACAGTAAGCATAAGGCACTTACCGCAAGCAGTGGATGGCACCAACTTTTAA AGGTGACTTTATTAATCAATGGTTTCACCGCTAAATTATATTTTTACAGATATGCACCTATGCAACTTAA TGTGGCTCTTCTAAGCAGGTGAGGACCTCCTCCTCAATGCTCTATAAAGTGTTTGTGTTCTGTATAGTGA GTTTCCCAGTAAATGTGGCTTAATACTTTAATTCTTAGGATGTGTCTTTCTACGTGATGCAGCTACTAAA TTCTGTTTTGTTTGTGGAATGACTAGCACAGGCCGACGCCCTCTTTCCCTCACTTACCAGAAGACCAATG AGCTGTTAATGAGCGGTTATCTCCATGGTATTACTGCTAAATGCACTGATGTCATAAGTATGTGGAATCC TTTTCCTTTTGAGACTGTATATCATATACCAGACTGAATCTACTTAATAAACACTGAACAACAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30620653 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30620653 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17655 same embryo
	EMAGE:17651 same embryo
	EMAGE:17654 same embryo
	EMAGE:17653 same embryo
	EurExpress:euxassay_006045 same experiment
	MGI:4822793 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source