EMAGE mouse gene expression spatial database

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EMAGE:17667

Nes nestin ( MGI:101784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006000. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006000_01	euxassay_006000_02	euxassay_006000_03	euxassay_006000_04

euxassay_006000_05	euxassay_006000_06	euxassay_006000_07	euxassay_006000_08	euxassay_006000_09

euxassay_006000_10	euxassay_006000_11	euxassay_006000_12	euxassay_006000_13	euxassay_006000_14

euxassay_006000_15	euxassay_006000_16	euxassay_006000_17	euxassay_006000_18	euxassay_006000_19

euxassay_006000_20	euxassay_006000_21	euxassay_006000_22	euxassay_006000_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17667_wholemount_strong.wlz

17667_wholemount_moderate.wlz

17667_wholemount_weak.wlz

17667_wholemount_possible.wlz

17667_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17667_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23

foot mesenchyme

moderate

regional

moderate expression: see section 05 06 07 18 19 20

hip mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23

upper leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14

diencephalon meninges

moderate

regional

moderate expression: see section 08 14 15 16 17 18 19 weak expression: see section 09 10 11 12 13

telencephalon meninges

strong

regional

strong expression: see section 21 22 23 moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 weak expression: see section 09 10 11 12 13

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 14 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 06 07 08 14 15 16 17 18 19 20 21 weak expression: see section 09 10 11 12 13

midbrain meninges

moderate

regional

moderate expression: see section 07 08 14 15 16 17 18 19 20 21 weak expression: see section 09 10 11 12 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

spinal cord meninges

moderate

regional

moderate expression: see section 14 15 16 17 weak expression: see section 10 11 12 13

tongue

strong

regional

strong expression: see section 09

tongue muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

kidney calyx

strong

regional

strong expression: see section 07 08 moderate expression: see section 09 17 18

tail mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T120
Entity Detected:	Nes, nestin ( MGI:101784)
Sequence:	sense strand is shown >T120 AGGATGCTGATGAAGAAGAAGGAGCAGAGTCAGGGACTCAGTGGTGGGGGCCAGGGCCCTCTGGTGGAGG TGTCAAGGTCCAGGATGTCACCCAGAGAGGGGACCTGGAACATGAATCTGTGGGTGACAGTGGCCTCTGG GATGATGGCTTGAGTGGGGCTGCAGCTAATGTTCTTGTAACTGCCCTAGAGACGGTGTCTCAGGACAGTG CTGAGCCTTCCGGGTCAGAGGGGTCTGAGTCTGCTTCCTTGGAGGGGGAGGAAGGTCAAGCGATTGACCA TTTAGATGCCCCCCAAGAGGTGACTAGCGTGGTCCCAGGGGCAGGAGACACCTTTGATATCAGTGGCCAG GGCCCCAACCTGGAGTCAGAGCAAGTGAATGGGAGGATGGAGAATGGACTAGAGCAGGCTGAGGGTCAGG TGGTTCTGCATGGGGACGAGGATCAAGGCATCCCTTTACAGGAACAGGGTACCCTCAAGGCCCCTTTAGT AGGGTCTCCTGTGCATCTAGGCCCAAGC
Notes:	The probe template was PCR amplified from IMAGE:614992 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:614992 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17666 same embryo
	EMAGE:17665 same embryo
	EurExpress:euxassay_006000 same experiment
	MGI:4826668 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source