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EMAGE:17685

Perp PERP, TP53 apoptosis effector ( MGI:1929938)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685
"Pseudo-wholemount" of euxassay_006025. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006025_01 euxassay_006025_02 euxassay_006025_03 euxassay_006025_04
EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685
euxassay_006025_05 euxassay_006025_06 euxassay_006025_07 euxassay_006025_08 euxassay_006025_09
EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685
euxassay_006025_10 euxassay_006025_11 euxassay_006025_12 euxassay_006025_13 euxassay_006025_14
EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685
euxassay_006025_15 euxassay_006025_16 euxassay_006025_17 euxassay_006025_18 euxassay_006025_19
EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685 EMAGE:17685
euxassay_006025_20 euxassay_006025_21 euxassay_006025_22 euxassay_006025_23 euxassay_006025_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17685Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17685_wholemount_strong.wlz
17685_wholemount_moderate.wlz
17685_wholemount_weak.wlz
17685_wholemount_possible.wlz
17685_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17685_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 10 11 12 13 14
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 09 16 17 18 19
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 21 22 23
lateral ventricle choroid plexus
weak weak
regionalweak expression: see section 06 07 08 09 10 11 13 14 16 17 18 19
4th ventricle lateral recess
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 16 17 18 21 22 23 24 moderate expression: see section 19 20
cornea epithelium
strong strong
regionalstrong expression: see section 01 02 03 04 05 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 moderate expression: see section 18 19
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 13 14 15 16 17
heart ventricle
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15 16
esophagus
strong strong
regionalstrong expression: see section 10 11 12
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07
rectum
strong strong
regionalstrong expression: see section 12 13 14
lower jaw incisor
strong strong
regionalstrong expression: see section 12 13 14 15 16 17
oral epithelium
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
upper jaw incisor
strong strong
regionalstrong expression: see section 13 17
bladder
strong strong
regionalExpression in the fundus region.
urethra of male
strong strong
regionalstrong expression: see section 12 13 14
trachea
strong strong
regionalstrong expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T280
Entity Detected:Perp, PERP, TP53 apoptosis effector ( MGI:1929938)
Sequence:sense strand is shown

>T280
CTGCTCCTGCCCGGCGTTCCTCCGCTCCGCGCCCGCCGCCACCGACGACATGCTGCGCTGCGGCCTGGCC
TGCGAGCGCTGCAGGTGGATCCTGCCCCTGCTGCTGCTCAGCGCCATCGCCTTCGACATCATCGCGCTGG
CCGGCCGTGGCTGGCTGCAGTCTAGCAACCACATCCAGACATCGTCGCTTTGGTGGAGGTGTTTCGACGA
GGGCGGCGGCAGCGGCTCCTACGACGATGGCTGCCAGAGCCTCATGGAGTACGCATGGGGACGAGCAGCT
GCGGCCACGCTCTTCTGTGGCTTTATCATCCTGTGCATCTGCTTCATTCTCTCGTTCTTCGCCCTGTGTG
GACCCCAGATGCTTGTTTTCCTGAGAGTTATTGGAGGCCTCCTCGCACTGGCTGCCATATTCCAGATCAT
CTCCCTGGTAATCTACCCCGTGAAGTACACACAGACCTTCAGGCTTCACGATAACCCTGCTGTTAATTAC
ATCTATAA
Notes:The probe template was PCR amplified from IMAGE:2655672 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655672 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17686 same embryo
 EMAGE:17687 same embryo
 EurExpress:euxassay_006025 same experiment
 MGI:4827157 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS