EMAGE mouse gene expression spatial database

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EMAGE:17730

Kbtbd11 kelch repeat and BTB (POZ) domain containing 11 ( MGI:1922151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008912. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008912_01	euxassay_008912_02	euxassay_008912_03	euxassay_008912_04

euxassay_008912_05	euxassay_008912_06	euxassay_008912_07	euxassay_008912_08	euxassay_008912_09

euxassay_008912_10	euxassay_008912_11	euxassay_008912_12	euxassay_008912_13	euxassay_008912_14

euxassay_008912_15	euxassay_008912_16	euxassay_008912_17	euxassay_008912_18	euxassay_008912_19

euxassay_008912_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17730_wholemount_strong.wlz

17730_wholemount_moderate.wlz

17730_wholemount_weak.wlz

17730_wholemount_possible.wlz

17730_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17730_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 08 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19

spinal cord

strong

regional

strong expression: see section 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 17

cervical ganglion

strong

regional

strong expression: see section 10 18

thoracic ganglion

strong

regional

strong expression: see section 16 moderate expression: see section 13 14

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 16 17 18 19

retina

strong

regional

strong expression: see section 01 02 03

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35395
Entity Detected:	Kbtbd11, kelch repeat and BTB (POZ) domain containing 11 ( MGI:1922151)
Sequence:	sense strand is shown >T35395 GGAGTGGCACTATTAGGAGGTGTGGCCTTATTGGAATGGGTAGTCACTGTGGGCATGGTCTTAAGACCCT CATCCCAGCTGCCTGGAAGCCAGTCTTCTAGCAGCCTCTAGGTGAAGATGTAGAACTCTCAGCTCCTCCT GCACCGTGCCTGCCTGGATGCTGCCATGCACCCACATTGATGACAAAGCACTGAGCCTCTGAACCTGTAA GCCAGCCCCAGTTAAATGTGGTCCTTATAAGACTTGCCTTGGCCATGGTGTCTGCTCACAACAGTGAAGC CCTAACTAAGACACTGCTGCTCAGCTCAGCTCCCTGTCTCCATTTCCAGGACCCAGTCAGGAAATGCTGC TACCATAGCGAGCAGCTCTTTCCCTTCTAATTAATGTAATCAAGGCAACTCCTCACAGGCATGCTCAGAG ACCTATGTTAACACCCACCTTTGCCCCCACAACAAGTGGGAGTGAATTTGTTAAGGTGTTAGTTTGGTAG TGAGGGGAGTGAGCTTTGCCGAGATGTGGCTCCAGTGTGTGAACCAGGTAGGTGCTTCCTACACATGGGT CCCATTCTGCCTCTTAACTCCACCTAGGAGTTTGGTTGGCATGAGCACAGATCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81183. Forward Primer - name:081183_F_cDNA_4930465M17Rik, sequence:GGAGTGGCACTATTAGGAGGTG; Reverse Primer - name:081183_N_SP6_cDNA_4930465M17Rik, sequence:AGATCTGTGCTCATGCCAACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17731 same embryo
	EMAGE:17734 same embryo
	EMAGE:17732 same embryo
	EMAGE:17733 same embryo
	EurExpress:euxassay_008912 same experiment
	MGI:4825692 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source