EMAGE mouse gene expression spatial database

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EMAGE:17835

Galnt14 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 ( MGI:1918935)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009033. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009033_01	euxassay_009033_02	euxassay_009033_03	euxassay_009033_04

euxassay_009033_05	euxassay_009033_06	euxassay_009033_07	euxassay_009033_08	euxassay_009033_09

euxassay_009033_10	euxassay_009033_11	euxassay_009033_12	euxassay_009033_13	euxassay_009033_14

euxassay_009033_15	euxassay_009033_16	euxassay_009033_17	euxassay_009033_18	euxassay_009033_19

euxassay_009033_20	euxassay_009033_21	euxassay_009033_22	euxassay_009033_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17835_wholemount_strong.wlz

17835_wholemount_moderate.wlz

17835_wholemount_weak.wlz

17835_wholemount_possible.wlz

17835_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17835_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

single cell

moderate expression: see section 11 12 13 14 15

pituitary gland

moderate

regional

moderate expression: see section 09 10 11 12 13 14

telencephalon

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08

hindbrain

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 17

midbrain

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 05 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 07 08 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 06 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 16

spinal cord

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 10 11 12 13 weak expression: see section 14

neural retina

weak

regional

weak expression: see section 02 03 04

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 10 11 12 13 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36414
Entity Detected:	Galnt14, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 ( MGI:1918935)
Sequence:	sense strand is shown >T36414 AGGAGATGGAGCAAAGTCTCAGGTGTGGGCCTTCACATACACCCAGCAGATCATCCAAGAAGAGCTGTGC CTGTCCGTGGTCACCCTGTTTCCTGGTGCCCCCGTGGTTCTTGCTCTATGCAAGAATGGGGATGAAAGGC AGCTATGGACCAAGACTGGTGCCCGAATTGAGCACATAGCATCTCACCTCTGCCTGGATACAGACATGTT TGGTGACAGCACCGAGGATGGCAAGGAGGTCGTTGTCAACCCTTGTGAGTCGTCGCTCATGAGCCAGCAC TGGGACATTGTGAGCTCATGAGGACCCTGGGTGCATTCAACTGTGGACCAGGAATGATGCTTCCCTGGAC CAAACCAAACTGGAACCCAGGTTGTGAACAGCTCATGACTGCACCCGATGTCCTCGACTGGAGGTAGAGC AAAGGCTCCCAGGACAGGAGCAACAGTCTCAGGGAAGATGGAGGAAAAGGTCACAAACCAGCAGGGCTCA GTGACACCCTCATGTGTGCAATATTATGAAGGTCCAGTCCTGGCCAGGTTTTCTGATTGGGACCTGGAGA TGGAGATCCGATGGGAAGTGTTTGTTGTACCCTTTCTTACAAAACAAGACACCTTATGGAGGCTTGTCTT GAAAGCTGTCCTTGTCACTGTGCCAGGACGACATGGAGAGATGCACAATAGAGATGGTTTACAGAACAAA TAAAGCTAACTTTGGGAATTGTCTTCGATAACTCATGCTGCTTCTTCAGCTAGCTGGGCACCTCATCTGA GCTCCCCTCTGAATGTGGGTGGGGCGTCTTGTGGTAGAATGAATGAAGAAGACAGGAAGATGGAAACCTT GTGTCCTTGTCCTGGGTTACCACCTACAAGCTATGTGATCTTTAGAATGATACTTTAGGGAGCTGGCAAG ATGGCTCAGTGGGTAAGGGTGCTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85546. Forward Primer - name:085546_F_cDNA_Galnt14, sequence:AGGAGATGGAGCAAAGTCTCAG; Reverse Primer - name:085546_N_SP6_cDNA_Galnt14, sequence:CAAGCACCCTTACCCACTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17838 same embryo
	EMAGE:17839 same embryo
	EMAGE:17837 same embryo
	EMAGE:17836 same embryo
	EMAGE:17834 same embryo
	EurExpress:euxassay_009033 same experiment
	MGI:4824982 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source