EMAGE mouse gene expression spatial database

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EMAGE:17925

Il17rd interleukin 17 receptor D ( MGI:2159727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011690. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011690_01	euxassay_011690_02	euxassay_011690_03	euxassay_011690_04

euxassay_011690_05	euxassay_011690_06	euxassay_011690_07	euxassay_011690_08	euxassay_011690_09

euxassay_011690_10	euxassay_011690_11	euxassay_011690_12	euxassay_011690_13	euxassay_011690_14

euxassay_011690_15	euxassay_011690_16	euxassay_011690_17	euxassay_011690_18	euxassay_011690_19

euxassay_011690_20	euxassay_011690_21	euxassay_011690_22	euxassay_011690_23	euxassay_011690_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17925_wholemount_strong.wlz

17925_wholemount_moderate.wlz

17925_wholemount_weak.wlz

17925_wholemount_possible.wlz

17925_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17925_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

moderate

regional

moderate expression: see section 15 weak expression: see section 13

olfactory cortex ventricular layer

weak

regional

weak expression: see section 12 13 17 18

telencephalon ventricular layer

strong

regional

strong expression: see section 14 moderate expression: see section 15 weak expression: see section 11 12 13 16 17 18

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 07 08 09 11 12

midbrain ventricular layer

strong

regional

strong expression: see section 07 11 12 moderate expression: see section 06 08 13 14 15 weak expression: see section 09 10

spinal cord ventricular layer

weak

regional

weak expression: see section 08

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 17 18 19

vomeronasal organ

weak

regional

weak expression: see section 12

lower jaw incisor

weak

regional

weak expression: see section 10 11 15

upper jaw incisor

weak

regional

weak expression: see section 11 12 15 16

metanephros

moderate

regional

moderate expression: see section 04 weak expression: see section 05 06 07 15 16

lung

moderate

regional

moderate expression: see section 03 04 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36502
Entity Detected:	Il17rd, interleukin 17 receptor D ( MGI:2159727)
Sequence:	sense strand is shown >T36502 CCAGCCGACTCTTATTCATACCTGGAGAGTCAGCATGTAGGCCTGGACCAAGACACTGAGGCCCAGCCCT CCTGTGATAGTGCCCCTGCCTTGCAGCCCCTGTTACACGCAGTGAAAGCTGGCAGTCCCTCAGAGATGCC ACGGGACTCAGGCATATATGATTCTTCTGTACCCTCATCAGAGCTCTCTCTGCCTCTGATGGAGGGACTC TCTCCGGATCAGATAGAAACATCTTCTCTGACCGAGAGTGTATCTTCCTCCTCTGGCCTAGGTGAGGAGG ACCCCCCTACCCTCCCTTCCAAGCTCCTTGCCTCTGGGGTGTCCAGAGAACATGGTTGCCACAGCCACAC TGACGAACTGCAAGCGCTTGCTCCTTTGTAAGGACTCGGAAGAGTCTAAGCATCGCCACTTTAGCTGCTG ATCTCTCTGGCTCCCCAGTTCACCTCTGTGGTTGTGCAGCCTACTTGGAGCTGAAGGCGCACACGGGGAT ATCTGGAATGAAATTCTGGCTAGCACTCGATCTGTTCTGCTCCAGCCTTCTAAGGGATACTTAGCAAAGT CTCCAGTTTCCCAAAAGCATATGGAACCCTGAAAGGCATGTCCATTAGATCTGACTGCAGCTGTCCATCT CAGACTGATCCTTGGATCTGAGCCTGCTCTGAGAGGGGTAGAGTGGAAACAGGTAACGAGAAATAGGAAA GCTCCCACACAGCCTGCTCTGGCTTCACTTGAACTCTGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92454. Forward Primer - name:092454_F_cDNA_Il17rd, sequence:CCAGCCGACTCTTATTCATACC; Reverse Primer - name:092454_N_SP6_cDNA_Il17rd, sequence:GCCAGAGTTCAAGTGAAGCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17926 same embryo
	EMAGE:17924 same embryo
	EMAGE:17928 same embryo
	EMAGE:17927 same embryo
	EurExpress:euxassay_011690 same experiment
	MGI:4825571 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source