EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17931

Stk32b serine/threonine kinase 32B ( MGI:1927552)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011693. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011693_01	euxassay_011693_02	euxassay_011693_03	euxassay_011693_04

euxassay_011693_05	euxassay_011693_06	euxassay_011693_07	euxassay_011693_08	euxassay_011693_09

euxassay_011693_10	euxassay_011693_11	euxassay_011693_12	euxassay_011693_13	euxassay_011693_14

euxassay_011693_15	euxassay_011693_16	euxassay_011693_17	euxassay_011693_18	euxassay_011693_19

euxassay_011693_20	euxassay_011693_21	euxassay_011693_22	euxassay_011693_23	euxassay_011693_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17931_wholemount_strong.wlz

17931_wholemount_moderate.wlz

17931_wholemount_weak.wlz

17931_wholemount_possible.wlz

17931_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17931_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19

foot

weak

regional

weak expression: see section 04 06 20 21

hip

weak

regional

weak expression: see section 15

fibula

weak

regional

weak expression: see section 03 23

tibia

weak

regional

weak expression: see section 03 23

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 19 20 21 22 23 24 weak expression: see section 02 09 10 11 12 16 17 18

olfactory cortex marginal layer

weak

regional

weak expression: see section 11 12 16 17 18

ventral grey horn

weak

regional

weak expression: see section 07 08 09 10 11 12

otic capsule

weak

regional

weak expression: see section 07 08 09 17 18 19

nasal septum

weak

regional

weak expression: see section 12 13 16 17

thyroid cartilage

weak

regional

weak expression: see section 10 11 12

trachea

weak

regional

weak expression: see section 11 12

exoccipital bone

weak

regional

weak expression: see section 01 02 03 04 19 20 22 23 24

temporal bone petrous part

weak

regional

weak expression: see section 01 02 03 04 19 20 21 22 23 24

vault of skull

weak

regional

weak expression: see section 01 02

orbito-sphenoid

weak

regional

weak expression: see section 02 24

viscerocranium

weak

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37089
Entity Detected:	Stk32b, serine/threonine kinase 32B ( MGI:1927552)
Sequence:	sense strand is shown >T37089 GCAGATATGTAAACTCCTGGGCATTGATCAAGTGTTTATACATGTAAATTATCTCAATATCTAAAGTGTC CTCCCTAGCCCCCACACAATTTTAACCATGAGAACTTTGCAATTTGGCCCATCCTAGTTTGCTTGATGGA ACTTTCAACAACATAAATAGCTGATATGTTTGTCCCTCTTATGGGACTTCCAGGTTCCAAAGCAACCAGG AAGAGTCACACACAGACTTCCTGGCCTTCTGGAATGGTGCAGCATAAAGACCAAGCCCAGGAGTTTCTGC TGCAAACAGGTTTTTCCCTGACTCGTGGAAGGCTGCCCCCACATTTGGATTCCAGCAATCACCAGCTATC ACCATATCCCCACATCCCCGTGTTAGATGGCACTGTGTCTCTGGCCACTCCTCCCCACCACAGAGTGTCC TGGGATCCCAGAGGAGACTCATCCTGCAGCGTGACCTCAGTCCCCACCAGGAAGAATATAAGAGTCCAGT CAGCCTGTCATGCAAAGGCTCCCTGGCTGGGTAGTCAGAGCTTCCTGTTGATACCTTAGGAAGCCTCGGT GCTTAGAGTCTTCTGTAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91036. Forward Primer - name:091036_F_cDNA_Stk32b, sequence:GCAGATATGTAAACTCCTGGGC; Reverse Primer - name:091036_N_SP6_cDNA_Stk32b, sequence:TCTACAGAAGACTCTAAGCACCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17930 same embryo
	EMAGE:17929 same embryo
	EurExpress:euxassay_011693 same experiment
	MGI:4828501 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source