EMAGE mouse gene expression spatial database

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EMAGE:17966

Fam59a family with sequence similarity 59, member A ( MGI:2685790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011729. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011729_01	euxassay_011729_02	euxassay_011729_03	euxassay_011729_04

euxassay_011729_05	euxassay_011729_06	euxassay_011729_07	euxassay_011729_08	euxassay_011729_09

euxassay_011729_10	euxassay_011729_11	euxassay_011729_12	euxassay_011729_13	euxassay_011729_14

euxassay_011729_15	euxassay_011729_16	euxassay_011729_17	euxassay_011729_18	euxassay_011729_19

euxassay_011729_20	euxassay_011729_21	euxassay_011729_22	euxassay_011729_23	euxassay_011729_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17966_wholemount_strong.wlz

17966_wholemount_moderate.wlz

17966_wholemount_weak.wlz

17966_wholemount_possible.wlz

17966_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17966_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 11 15 16

medulla oblongata floor plate

moderate

regional

moderate expression: see section 12

metencephalon floor plate

moderate

regional

moderate expression: see section 12

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

spinal cord

moderate

spotted

moderate expression: see section 09

spinal cord floor plate

moderate

regional

moderate expression: see section 12

heart valve

strong

spotted

strong expression: see section 13

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 14 15 16

tongue muscle

moderate

spotted

moderate expression: see section 11 12 13 14 15 16

metanephros

moderate

spotted

moderate expression: see section 06 07 08 09 17 18 19

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37725
Entity Detected:	Fam59a, family with sequence similarity 59, member A ( MGI:2685790)
Sequence:	sense strand is shown >T37725 CCCATGATATCCTTCCCTATCAAGACTCTGGTGACAGTGGGAGCGACTACCTTTTCCCGGAAGCTAATGA GGAGTCAGCGGGCATCCCGGGGAAAACAGAAGTTCCCTATGAAGAGCTGTGGCTGGAGGAAGGCAAGCCC AGCCGCCAGCCTCTCACTCGCTCACTCAGTGAGAAGAGCAGGTGCGACACCTTGAGAGGCTCCACGAGGT CCACGTGTGCACCCTCTTCTCCTCCCACCCCTGCGACCCTGGGAGCCACAATTAAGTCTTCAGAAATCGC CCTACCTCCACCTCCAGTGCCTCCCAAATCTGAAGCTGTCAGGGAAGAATGCCGGCTCCTGAACGCCCCA CCTGTGCCGCCCCGAAGTGCAAAGCCTTTGTCCACAAGTCCCTCCATCCCTCCTCGAACAGTCAAGCCAG TCCGGCCACAGACGAGATCTCCAAGCCCCACCCTGTCTTACTATTCTTCAGGACTGCATAACATCATCAC TCAGAGTGATACAAGTCCTCCCAACAGTGCCCCTGTGTCCTGCTACCCCTGCACCCGAGTGAAGAGTGAC TCTGTGGACCCCAAGTCCCCATTTGGAAGTCCTTCCGCTGAAGCTCTGTCCTCCCGGCTCTCATGGCCCA ACCATTACTCAGGAGCATCGGAGAACCAGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85701. Forward Primer - name:085701_F_cDNA_Gm944, sequence:CCCATGATATCCTTCCCTATCA; Reverse Primer - name:085701_N_SP6_cDNA_Gm944, sequence:GTCTGGTTCTCCGATGCTCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17965 same embryo
	EMAGE:17967 same embryo
	EMAGE:17968 same embryo
	EurExpress:euxassay_011729 same experiment
	MGI:4824749 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source