EMAGE mouse gene expression spatial database

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EMAGE:17970

Gpc5 glypican 5 ( MGI:1194894)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011731. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011731_01	euxassay_011731_02	euxassay_011731_03	euxassay_011731_04

euxassay_011731_05	euxassay_011731_06	euxassay_011731_07	euxassay_011731_08	euxassay_011731_09

euxassay_011731_10	euxassay_011731_11	euxassay_011731_12	euxassay_011731_13	euxassay_011731_14

euxassay_011731_15	euxassay_011731_16	euxassay_011731_17	euxassay_011731_18	euxassay_011731_19

euxassay_011731_20	euxassay_011731_21	euxassay_011731_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17970_wholemount_strong.wlz

17970_wholemount_moderate.wlz

17970_wholemount_weak.wlz

17970_wholemount_possible.wlz

17970_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17970_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot

strong

regional

strong expression: see section 20 moderate expression: see section 03 04 05 19 21

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 19 20 21 22

olfactory cortex marginal layer

strong

single cell

strong expression: see section 10 11 12 13 15 16 17 18

medulla oblongata alar plate mantle layer

strong

single cell

strong expression: see section 08 09 10 11 12 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 10 11 12 14 15 16

rest of cerebellum mantle layer

strong

single cell

strong expression: see section 10 11 12 14 15 16

pons mantle layer

strong

single cell

strong expression: see section 10 11 12 14 15 16

midbrain mantle layer

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord mantle layer

strong

single cell

strong expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37728
Entity Detected:	Gpc5, glypican 5 ( MGI:1194894)
Sequence:	sense strand is shown >T37728 TCCACACTGGCATGCTTATATCCGGTCTTTGGAAGAGTTGTCAGATGCGATGCACGGAACCTACGACGTT GAACACGTGCTCCTGAACTTCCACTTGCTTGTTAATGATGCCGTGTTGCAGGCTCACCTCAATGGGCAGA AATTATTGGATCAGGTGAATACGATTTGTGGCCATCCAGTGAGAACACCAACACAGAGCCCCCGCTGTAC TTTTGACCCAAGCAAAGAGAAACATGGAATGAAGATCTCTGCAAGGAATGGTGAAGAGACACTTGCCAAC AGAAGAAAAGAATTCATCAATAGCCTTAGGCTGCATGGGTCCTTCTATGGTGGCCTGGCTGACCAGCTTT GTGTTAATGAATTGGCTGCCCCTGAAGGAAGGCCCTGCTGGAATGGGGAGGAAATAGTCAAAAGCTATGC TCAGCGTGTGGTTGGGAATGGAATCAAAGCCCAGTCTGCAAATCCTGAAGTCAGAGTCAGAGGAACCGAT CCTGTGGTAAATCAGATCATTGATAAACTGAAGCATGTCATCCAGTTGTTACGGGGGAGATCACCCAAGC CCAACAAGTGGGAGCTCCTTCAGCCAGGCAGTGGAGGCGGCATGCTTGAGAACTCTAGTGGAGACTGTGA TGACGAAGATGGCTGTGGAGGATCAGGAAGCGGAGAAGTAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68777. Forward Primer - name:068777_F_cDNA_Gpc5, sequence:TCCACACTGGCATGCTTATATC; Reverse Primer - name:068777_N_SP6_cDNA_Gpc5, sequence:TTACTTCTCCGCTTCCTGATCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17971 same embryo
	EMAGE:17972 same embryo
	EMAGE:17969 same embryo
	EurExpress:euxassay_011731 same experiment
	MGI:4825158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source