EMAGE mouse gene expression spatial database

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EMAGE:18020

Hpse heparanase ( MGI:1343124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011782. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011782_01	euxassay_011782_02	euxassay_011782_03	euxassay_011782_04

euxassay_011782_05	euxassay_011782_06	euxassay_011782_07	euxassay_011782_08	euxassay_011782_09

euxassay_011782_10	euxassay_011782_11	euxassay_011782_12	euxassay_011782_13	euxassay_011782_14

euxassay_011782_15	euxassay_011782_16	euxassay_011782_17	euxassay_011782_18	euxassay_011782_19

euxassay_011782_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18020_wholemount_strong.wlz

18020_wholemount_moderate.wlz

18020_wholemount_weak.wlz

18020_wholemount_possible.wlz

18020_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18020_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

homogeneous

weak expression: see section 01 02 03 04 05 14 15 16 17 18 19 20

humerus

weak

homogeneous

weak expression: see section 01 02 03 04 18 19 20

femur

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 18 19 20

otic capsule

weak

homogeneous

weak expression: see section 05 06

viscerocranium

weak

homogeneous

Expression in the turbinate bone.

meckel's cartilage

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 12 16 17 18 19

axial skeleton

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15

basioccipital bone

weak

homogeneous

weak expression: see section 01 02 03 15 16 17 18 19

basisphenoid bone

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12

temporal bone petrous part

weak

homogeneous

weak expression: see section 01 02 16 17 18 19 20

vault of skull

weak

homogeneous

weak expression: see section 18 19

orbito-sphenoid

weak

homogeneous

weak expression: see section 01 02 03 04 05 16 17 18 19 20

clavicle

weak

homogeneous

weak expression: see section 05 06 16 17

scapula

weak

homogeneous

weak expression: see section 01 02 18 19

pelvic girdle skeleton

weak

homogeneous

weak expression: see section 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37739
Entity Detected:	Hpse, heparanase ( MGI:1343124)
Sequence:	sense strand is shown >T37739 CGAGAGCAGTACCAAAAGGAGTTCAAGAACAGCACCTACTCAAGAAGCTCAGTGGACATGCTCTACAGTT TTGCCAAGTGCTCGGGGTTAGACCTGATCTTTGGTCTAAATGCGTTACTACGAACCCCAGACTTACGGTG GAACAGCTCCAACGCCCAGCTTCTCCTTGACTACTGCTCTTCCAAGGGTTATAACATCTCCTGGGAACTG GGCAATGAGCCCAACAGTTTCTGGAAGAAAGCTCACATTCTCATCGATGGGTTGCAGTTAGGAGAAGACT TTGTGGAGTTGCATAAACTTCTACAAAGGTCAGCTTTCCAAAATGCAAAACTCTATGGTCCTGACATCGG TCAGCCTCGAGGGAAGACAGTTAAACTGCTGAGGAGTTTCCTGAAGGCTGGCGGAGAAGTGATCGACTCT CTTACATGGCATCACTATTACTTGAATGGACGCATCGCTACCAAAGAAGATTTTCTGAGCTCTGATGTGC TGGACACTTTTATTCTCTCTGTGCAAAAAATTCTGAAGGTCACTAAAGAGATCACACCTGGCAAGAAGGT CTGGTTGGGAGAGACGAGCTCAGCTTACGGTGGCGGTGCACCCTTGCTGTCCAACACCTTTGCAGCTGGC TTTATGTGGCTGGATAAATTGGGCCTGTCAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95604. Forward Primer - name:095604_F_cDNA_Hpse, sequence:CGAGAGCAGTACCAAAAGGAGT; Reverse Primer - name:095604_N_SP6_cDNA_Hpse, sequence:GGCTGACAGGCCCAATTTATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18022 same embryo
	EMAGE:18019 same embryo
	EMAGE:18021 same embryo
	EurExpress:euxassay_011782 same experiment
	MGI:4825448 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source