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EMAGE:18092

Cldn4 claudin 4 ( MGI:1313314)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092
"Pseudo-wholemount" of euxassay_011852. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011852_01 euxassay_011852_02 euxassay_011852_03 euxassay_011852_04
EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092
euxassay_011852_05 euxassay_011852_06 euxassay_011852_07 euxassay_011852_08 euxassay_011852_09
EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092
euxassay_011852_10 euxassay_011852_11 euxassay_011852_12 euxassay_011852_13 euxassay_011852_14
EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092 EMAGE:18092
euxassay_011852_15 euxassay_011852_16 euxassay_011852_17 euxassay_011852_18 euxassay_011852_19
EMAGE:18092
euxassay_011852_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18092Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18092_wholemount_strong.wlz
18092_wholemount_moderate.wlz
18092_wholemount_weak.wlz
18092_wholemount_possible.wlz
18092_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18092_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
sublingual gland primordium
strong strong
regionalstrong expression: see section 07 moderate expression: see section 12 13
pancreas
weak weak
regionalweak expression: see section 06 07
pituitary gland
weak weak
regionalweak expression: see section 08 09 10
epidermis
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20 weak expression: see section 01 02 03 04 05 06 07 08 09 10
outer ear
strong strong
regionalstrong expression: see section 18 19 moderate expression: see section 01 02 03 16 17 not examined expression: see section 15
inner ear
moderate moderate
regionalmoderate expression: see section 04 15 16
pharyngo-tympanic tube
weak weak
regionalweak expression: see section 01 02 03 04 05 06 15 16 17 18 19 20
cornea epithelium
weak weak
regionalweak expression: see section 01 02 03 04 18 19 20
naris
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 10
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 12 13 14 weak expression: see section 08 09 10
esophagus
weak weak
regionalweak expression: see section 08 09 10
stomach
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 04 weak expression: see section 05 06 07
hindgut
moderate moderate
regionalmoderate expression: see section 09 10
rectum
moderate moderate
regionalmoderate expression: see section 09 10
midgut
moderate moderate
regionalmoderate expression: see section 08 09 12 13 weak expression: see section 07 10 11
bladder
strong strong
regionalExpression in the fundus region.
metanephros
moderate moderate
regionalmoderate expression: see section 05 06 07 12 13
male reproductive system
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 09
urethra of male
strong strong
regionalstrong expression: see section 09 10
larynx
weak weak
regionalweak expression: see section 09 10
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36163
Entity Detected:Cldn4, claudin 4 ( MGI:1313314)
Sequence:sense strand is shown

>T36163
CAAGATGTACGACTCGATGCTCGCCCTGCCGCAGGACCTGCAGGCCGCCCGAGCCCTTATGGTCATCAGC
ATCATCGTGGGTGCTCTGGGGATGCTTCTCTCAGTGGTAGGGGGCAAGTGCACCAACTGCATGGAGGACG
AGACCGTCAAGGCCAAGATCATGATCACCGCCGGAGCCGTGTTCATCGTGGCAAGCATGCTGATTATGGT
GCCCGTGTCCTGGACCGCTCACAACGTCATCCGCGACTTCTACAACCCTATGGTGGCTTCCGGGCAGAAG
AGGGAAATGGGGGCCTCGCTTTACGTCGGCTGGGCGGCCTCCGGGCTGCTGCTCCTGGGAGGAGGCCTCC
TCTGCTGCAGTTGCCCACCTCGTAGCAACGACAAGCCCTACTCGGCCAAGTACTCCGCCGCCCGCTCTGT
CCCCGCCAGCAACTATGTGTAAGGTGGGCCACTCTGTCCACATTGCCTTTGTTATTTTTTTTCGGATTGA
GCTCATAACAGCCTGTGGCCCCTCACATTCTCCAGGACCTGCCCTGCTATGGGCCACTAACTGCTTGCTG
GGGACAGGCAAACCCGGACTGTGCAAAGTTACTAGCCCGTAGCTCTTGGGCTGCTCCACATGGCTCCTTA
CGGCCGGCAAGAATGGATGTAAAAATATCTTGCTGCTTACATCCAAATTGCGGTGGATATGGGCTGAAGG
CAGAAGCAGCTGGGAAGGGCAGTAGAGGCGCAAGCTGGGTCCTGCTGGCCGGGGTAGCTCAGCTGTGACT
TTGGACTCGGAGTGGATGTCCTCATGTTAGCAAACGTCCACTGTCCTTTCTCTATCCCCCTCACTCAGCC
TACACGTTACTCCAGCGCTACTCTTGCCATTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74277. Forward Primer - name:074277_F_cDNA_Cldn4, sequence:CAAGATGTACGACTCGATGCTC; Reverse Primer - name:074277_N_SP6_cDNA_Cldn4, sequence:TAATGGCAAGAGTAGCGCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18094 same embryo
 EMAGE:18091 same embryo
 EMAGE:18090 same embryo
 EMAGE:18093 same embryo
 EMAGE:18095 same embryo
 EurExpress:euxassay_011852 same experiment
 MGI:4823913 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS