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EMAGE:18113

Ttc16 tetratricopeptide repeat domain 16 ( MGI:2443048)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113
"Pseudo-wholemount" of euxassay_011862. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011862_01 euxassay_011862_02 euxassay_011862_03 euxassay_011862_04
EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113
euxassay_011862_05 euxassay_011862_06 euxassay_011862_07 euxassay_011862_08 euxassay_011862_09
EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113
euxassay_011862_10 euxassay_011862_11 euxassay_011862_12 euxassay_011862_13 euxassay_011862_14
EMAGE:18113 EMAGE:18113 EMAGE:18113 EMAGE:18113
euxassay_011862_15 euxassay_011862_16 euxassay_011862_17 euxassay_011862_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18113Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18113_wholemount_strong.wlz
18113_wholemount_moderate.wlz
18113_wholemount_weak.wlz
18113_wholemount_possible.wlz
18113_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18113_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 13 14 15 16
humerus
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 01 02 03 17 18
hip
weak weak
regionalweak expression: see section 06 11
fibula
weak weak
regionalweak expression: see section 03 17
tibia
weak weak
regionalweak expression: see section 01 02 03 16 17 18
femur
weak weak
regionalweak expression: see section 01 02 03 13 14 15 16 17
otic capsule
weak weak
regionalweak expression: see section 05 12
meckel's cartilage
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 11 12 13 14 15
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 09 10 weak expression: see section 05 06 08 11 12
basioccipital bone
moderate moderate
regionalmoderate expression: see section 08 09 10 11
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12
exoccipital bone
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 01 02 03
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 01 02 03
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 14 15 weak expression: see section 01 02 04 05 06 16
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 01 02 03
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37181
Entity Detected:Ttc16, tetratricopeptide repeat domain 16 ( MGI:2443048)
Sequence:sense strand is shown

>T37181
GAGAAGGGCTTGTACATCAACCGTGGGGATTGCTTCTTCCAGCTGGGCAACCTGGCCTTCGCGGAGGCTG
ACTACAAGCAAGCGCTGGCCCTGAGCCCGCTGGATGAAGGAGCCAACTTGCGCATGGGTGTGCTGCAGGA
GAAGCTGGGCTTCTGCCAGCAGAAACACAGGCAGTTCCAGACAGCAGAGGAGCACTTCTCGGAGGCCATA
AGGCACAGCCCTCAGAAACCACAGTACTACCTGCACCGGGCCAAGTGCCGACAGTTCCTGCAGAACACTC
TGGGGGCCCGCCTGGATGTCGCTACTGTACTGCTTGTCAATCCCGAGTATCCCAAGATGGCTGCAGTGAT
GAACACCCTCTTTCCTAGCATGACTGTGGAGAATGTGCTTAAGTCCCAAGTGGCCGAACTGGCCAAGCTC
CAGCTAAGTCGGATGATAGAAAATGGGCCGAAGAACATCTACCCTCAGAGCACTGTGGTGCAGCGGTTAT
TGGAACGCAGGAAAGCCCAAGTCCTGGTGAAGCTCTGGAAGCAGGAACGTCTTGGGACCCCTGAAGAGGA
AGTGACTCTTTACCAGGCTCCGCAGCTGGCAGAGGAAAAAAAGGTGAAAACCGCTCGCAGAAGGACATCC
CTGACAGACAGCTATGCGGACCAGACCTCCTCAGGCTCCGTCTTTAGCATTGTGAGCATCAGCACGTCGG
GGCCAGAGATGTCAACCAGTCAGGAGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70097. Forward Primer - name:070097_F_cDNA_Ttc16, sequence:GAGAAGGGCTTGTACATCAACC; Reverse Primer - name:070097_N_SP6_cDNA_Ttc16, sequence:ACTCCTGACTGGTTGACATCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18111 same embryo
 EMAGE:18110 same embryo
 EMAGE:18112 same embryo
 EurExpress:euxassay_011862 same experiment
 MGI:4828978 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS