EMAGE mouse gene expression spatial database

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EMAGE:18141

Cnot7 CCR4-NOT transcription complex, subunit 7 ( MGI:1298230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011947. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011947_01	euxassay_011947_02	euxassay_011947_03	euxassay_011947_04

euxassay_011947_05	euxassay_011947_06	euxassay_011947_07	euxassay_011947_08	euxassay_011947_09

euxassay_011947_10	euxassay_011947_11	euxassay_011947_12	euxassay_011947_13	euxassay_011947_14

euxassay_011947_15	euxassay_011947_16	euxassay_011947_17	euxassay_011947_18	euxassay_011947_19

euxassay_011947_20	euxassay_011947_21	euxassay_011947_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18141_wholemount_strong.wlz

18141_wholemount_moderate.wlz

18141_wholemount_weak.wlz

18141_wholemount_possible.wlz

18141_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18141_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

homogeneous

weak expression: see section 01 02 03 17 18 19

humerus

weak

homogeneous

weak expression: see section 01 02

femur

weak

homogeneous

weak expression: see section 01 02 03 18

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12

dorsal root ganglion

weak

regional

weak expression: see section 05 06 12

nasal septum

weak

homogeneous

weak expression: see section 12

viscerocranium

weak

homogeneous

Expression in the turbinate bone.

meckel's cartilage

weak

homogeneous

weak expression: see section 02 03 05 06 07 08 09 10 11 13 14

axial skeleton

weak

regional

weak expression: see section 04 05 06 08 09 10 11 12 13

basioccipital bone

weak

homogeneous

weak expression: see section 01 02 03 16 17

basisphenoid bone

weak

homogeneous

weak expression: see section 07 08 09 10 11 12

temporal bone petrous part

weak

homogeneous

weak expression: see section 01

orbito-sphenoid

weak

homogeneous

weak expression: see section 01 02 03 06 07

scapula

weak

homogeneous

weak expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30016
Entity Detected:	Cnot7, CCR4-NOT transcription complex, subunit 7 ( MGI:1298230)
Sequence:	sense strand is shown >T30016 CAGCACCAGGCAGGATCTGACTCACTGCTTACAGGAATGGCCTTTTTCAAAATGAGAGAAATGTTCTTTG AAGATCACATTGATGATGCCAAATACTGTGGTCACTTATATGGCCTTGGTTCTGGCTCATCCTATGTACA GAACGGCACAGGGAATGCATATGAAGAGGAAGCCAGCAAGCAGTCATGACAGGAAATAGTCTTCTTATTT TTATTTTATACGAATTACACACATGCTTGTATATAGGTTTTATCATCCCTTAAATAATAGGCAATACCCC CCCCCTTTTGTAGACCTTCTCTGCTTTCAGTAATAAGTATGGCCTTTCTTAGCTATGATCTTAATAAATT AAAGTACATTCAGGTTAAATCTGGCCTTAATTTAATACACTTCTTATCAAGCTTGTGTGACAAAGCTACA TTACTGAATAATTTGATAAACTTTGCCTACTTGAGCTTGGTTTTGTTTTTCCCTTTTCCTAAATTAACTA GCATTGAGTGTAATTTATTTCCCTCTTCCATGCCTCTCCTTCTATTCTGCAGTACTTATAGGTATTTAAG ATTGTGGACCATCAAGTTTGCACTTTAGAGAGTGATTTCAATCCTCTATTTTATCAGAAATTGTGATTTT TCTTGCTCTTAGCTAGAAAAATAACCATCTACCACCTTTTTACAGTATTTGCTTGTTTTTGACATTCAGA GTACAGCACGTGCATTGTGCAAATTGTTCAACAGGAATAACAGAAAAAGACAAGGAACTACTGAAGAGCC TAGTAACTACTGTTTCTGTGTGTAGTGTTTAGCCTTCCAAGCACATCTCATGTCTGTTGGTTTCTAATTG GCATGCATAGGCTGCTATGTGACTGAAGAATTTCAAACCAGCTTTACACCCTTCAGGAAGAAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3590764), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 36415. Forward Primer - name:036415_F_IRAV12-15_F12_Cnot7, sequence:CAGCACCAGGCAGGATCT; Reverse Primer - name:036415_R_SP6_IRAV12-15_F12_Cnot7, sequence:GGGCTTCTTCCTGAAGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18144 same embryo
	EMAGE:18142 same embryo
	EMAGE:18143 same embryo
	EMAGE:18145 same embryo
	EurExpress:euxassay_011947 same experiment
	MGI:4823957 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source