EMAGE mouse gene expression spatial database

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EMAGE:18161

Nrf1 nuclear respiratory factor 1 ( MGI:1332235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011903. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011903_01	euxassay_011903_02	euxassay_011903_03	euxassay_011903_04

euxassay_011903_05	euxassay_011903_06	euxassay_011903_07	euxassay_011903_08	euxassay_011903_09

euxassay_011903_10	euxassay_011903_11	euxassay_011903_12	euxassay_011903_13	euxassay_011903_14

euxassay_011903_15	euxassay_011903_16	euxassay_011903_17	euxassay_011903_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18161_wholemount_strong.wlz

18161_wholemount_moderate.wlz

18161_wholemount_weak.wlz

18161_wholemount_possible.wlz

18161_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18161_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 16 17 18

fibula

moderate

homogeneous

moderate expression: see section 16 17 18

tibia

moderate

homogeneous

moderate expression: see section 16 17 18

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 13 15 16 17 18

otic capsule

moderate

homogeneous

moderate expression: see section 05 06 07 13 14

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 15

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 14 15 16 17 18

basisphenoid bone

moderate

homogeneous

moderate expression: see section 09 10 11 12 13

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 04 15 16 17 18

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 04 05 13 14 15 18

scapula

moderate

homogeneous

moderate expression: see section 02 03 18

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 06 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30037
Entity Detected:	Nrf1, nuclear respiratory factor 1 ( MGI:1332235)
Sequence:	sense strand is shown >T30037 CCCCCGAGGACACTTCTTATGATGACTCGGACATCCTCAACTCCACGGCAGCTGATGAGGTAACTGCCCA TCTGGCTGCTGCAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGGAAGAAACGGAAACGG CCTCATGTGTTTGAGTCTAATCCATCTATCCGAAAGAGACAGCAGACACGTTTGCTTCGGAAACTCAGAG CCACGTTGGATGAGTACACGACGCGAGTGGGACAGCAAGCGATTGTACTCTGCATCTCACCCTCCAAACC CAACCCTGTCTTCAAGGTGTTTGGCGCAGCACCTTTGGAGAATGTGGTGCGAAAGTACAAGAGCATGATC CTGGAAGACCTCGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAATTCAGAGCTGCCGC CTCTCACCATCGATGGGATTCCAGTCTCTGTGGACAAAATGACCCAGGCTCAGCTTCGGGCATTTATCCC AGAGATGCTCAAGTATTCCACAGGTCGGGGGAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCTATCTGG TGGCCAGAAGATATCCCATGGGCCAATGTCCGCAGTGATGTCCGCACAGAAGAGCAAAAACAAAGGGTTT CATGGACCCAGGCATTACGGACCATAGTTAAAAATTGCTATAAGCAACATGGGCGGGAGGATCTTTTATA TGCTTTTGAAGATCAGCAAACACAAACTCAGGCCACCACCACACACAGTATAGCTCATCTCGTACCATCA CAGACCGTAGTACAGACCTTCAGCAACCCTGATGGCACCGTGTCGCTCATCCAGGTTGGTACAGGGGCAA CAGTAGCCACATTGGCTGATGCTTCAGAACTGCCAACCACAGTCACTGTTGCCCAAGTGAATTACTCTGC TGTGGCTGATGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3496661), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10000. Forward Primer - name:010000_F_IRAV12-15_O15_Nrf1, sequence:CCCCCGAGGACACTTCTT; Reverse Primer - name:010000_R_SP6_IRAV12-15_O15_Nrf1, sequence:TCTCCATCAGCCACAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18163 same embryo
	EMAGE:18160 same embryo
	EMAGE:18164 same embryo
	EMAGE:18162 same embryo
	EurExpress:euxassay_011903 same experiment
	MGI:4826790 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source