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EMAGE:18165

Tnfaip1 tumor necrosis factor, alpha-induced protein 1 (endothelial) ( MGI:104961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165
"Pseudo-wholemount" of euxassay_011960. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011960_01 euxassay_011960_02 euxassay_011960_03 euxassay_011960_04
EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165
euxassay_011960_05 euxassay_011960_06 euxassay_011960_07 euxassay_011960_08 euxassay_011960_09
EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165
euxassay_011960_10 euxassay_011960_11 euxassay_011960_12 euxassay_011960_13 euxassay_011960_14
EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165
euxassay_011960_15 euxassay_011960_16 euxassay_011960_17 euxassay_011960_18 euxassay_011960_19
EMAGE:18165 EMAGE:18165 EMAGE:18165 EMAGE:18165
euxassay_011960_20 euxassay_011960_21 euxassay_011960_22 euxassay_011960_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18165Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18165_wholemount_strong.wlz
18165_wholemount_moderate.wlz
18165_wholemount_weak.wlz
18165_wholemount_possible.wlz
18165_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18165_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20 21
humerus
moderate moderate
homogeneousmoderate expression: see section 01 02 17 18 19 20 21 22
fibula
moderate moderate
homogeneousmoderate expression: see section 01 02 03 05
tibia
moderate moderate
homogeneousmoderate expression: see section 02 03 05
femur
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22
otic capsule
moderate moderate
homogeneousmoderate expression: see section 03 04 06 07 13 14
nasal septum
moderate moderate
homogeneousmoderate expression: see section 13 14
viscerocranium
moderate moderate
homogeneousExpression in the turbinate bone.
heart valve
moderate moderate
homogeneousmoderate expression: see section 08 09 11 12
meckel's cartilage
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
axial skeleton
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13
basioccipital bone
moderate moderate
homogeneousmoderate expression: see section 01 02 14 15 16 17
basisphenoid bone
moderate moderate
homogeneousmoderate expression: see section 07 08 09 10 11 12 13 14
temporal bone petrous part
moderate moderate
homogeneousmoderate expression: see section 01 16 17 18 19 20 21
vault of skull
moderate moderate
homogeneousmoderate expression: see section 01 02 22
orbito-sphenoid
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22
scapula
moderate moderate
homogeneousmoderate expression: see section 01 02 17 18
pelvic girdle skeleton
moderate moderate
homogeneousmoderate expression: see section 08 09 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30041
Entity Detected:Tnfaip1, tumor necrosis factor, alpha-induced protein 1 (endothelial) ( MGI:104961)
Sequence:sense strand is shown

>T30041
ACCCCTCGAGTCCCTGACAATTCCTTACTGGAGGCCACAAGCCGGAGCCGCAGCCAGGCTTCCCCCAGTG
AAGATGAAGACACCTTTGAACTACGGGACCGTGTCCGTCGCATTCATGTCAAGCGCTATAGCACTTACGA
TGACCGGCAACTTGGCCATCAGTCCACTCATCGTGACTGATGAGACCCTTAAGAAATCGGGGCACAAGAA
GTCCTGTCTGCCACCCTGTGGAACGTACCCATTGGCCACCCTATGCTGCTCCTGCTAGCTGGGAGCCTGC
TCTAGTATGTACAGGTGACAGCCCTGCTTGGGAGGTTGGGGAGCTGAGAGAGGGAAGGCCACATTGTCTT
GGCTGGCCCCCCTCCCCCCAGCACTTCCAGATGCCACCCCACTTCCTGCCAATAAGGAGCTACTTCTGCT
CTAGGGTGAGTGCACTGATTTCCCACCTCCTGCCAAGAATACCCCCTGGCCCTTGGCTGAGTGCCCCTTT
CTTCTGAAGAGCTCCTTTAGCTCCTCAGCTGAGGCCCACGAGAACAGCTTTGTTCTAGGGGAAAGGAGGC
TTCTGGTGCTGCGAGGACCCAATTTTGAACAGAAAAAGTGGCCTTTTTTTTTCCTAAGGTGTTTAAACAT
TGGTATAACCTGGATTTTTGTTTGTTTTTAAATCTAGATGTTAGCATGGCCGTACCTATAATCCCAGCTA
TGGAGAAGCCGAGGTGGGATAACATGTTCTAGGCTAGCCTGGACAACATAGCAAGGCCCTCAATATAAAA
TTTTAAAAAGGGGGCTCACCTAGCAAGAGTGAGGCCCTGGGCTCAATCTCCAGTACTGCCACACTCACTA
GGTATCTACGAAATGACTAGTTTTTAGTCTGGGGGGAAAGCTAGGACTAAGCAGAGTGCCAGGACCCCAC
CTAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3484017), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13564. Forward Primer - name:013564_F_IRAV01-03_M22_Tnfaip1, sequence:ACCCCTCGAGTCCCTGAC; Reverse Primer - name:013564_R_SP6_IRAV01-03_M22_Tnfaip1, sequence:ACTAGGTGGGGTCCTGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18167 same embryo
 EMAGE:18166 same embryo
 EMAGE:18168 same embryo
 EurExpress:euxassay_011960 same experiment
 MGI:4828839 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS