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EMAGE:18167

Il17rc interleukin 17 receptor C ( MGI:2159336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167
"Pseudo-wholemount" of euxassay_011899. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011899_01 euxassay_011899_02 euxassay_011899_03 euxassay_011899_04
EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167
euxassay_011899_05 euxassay_011899_06 euxassay_011899_07 euxassay_011899_08 euxassay_011899_09
EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167
euxassay_011899_10 euxassay_011899_11 euxassay_011899_12 euxassay_011899_13 euxassay_011899_14
EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167 EMAGE:18167
euxassay_011899_15 euxassay_011899_16 euxassay_011899_17 euxassay_011899_18 euxassay_011899_19
EMAGE:18167 EMAGE:18167
euxassay_011899_20 euxassay_011899_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18167Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18167_wholemount_strong.wlz
18167_wholemount_moderate.wlz
18167_wholemount_weak.wlz
18167_wholemount_possible.wlz
18167_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18167_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 05 06 07 11 12 13 14 15 16 17 18 weak expression: see section 03 04 19
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 15 16 17 18 19 20 21
hip
moderate moderate
regionalmoderate expression: see section 12 13 weak expression: see section 08
fibula
moderate moderate
regionalmoderate expression: see section 03 05
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 04
femur
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 15 16 17 18 19 20 weak expression: see section 07
otic capsule
weak weak
regionalweak expression: see section 05 06 07 11 12 13
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 weak expression: see section 10
axial skeleton
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 11 weak expression: see section 04 10
basioccipital bone
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 09 10
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 07 11 weak expression: see section 08 09 10
exoccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 13 14 15
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 14 15 16 17 18
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 05 07 15 16 17 19 20
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 01 02 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30038
Entity Detected:Il17rc, interleukin 17 receptor C ( MGI:2159336)
Sequence:sense strand is shown

>T30038
TGTGTCCGTGTGGTGGTCCACTTGGCCGTGCATGGGCACTGGGCAGAGCCTGAAGAAGCTGGAAAGTCTG
ATTCAGAACTCCAGGAGTCTAGGAACGCCTCTCTCCAGGCCCAGGTGGTGCTCTCCTTCCAGGCCTACCC
CATCGCCCGCTGTGCCCTGCTGGAGGTCCAGGTGCCCGCTGACCTGGTGCAGCCTGGTCAGTCCGTGGGT
TCTGCGGTATTTGACTGTTTCGAGGCTAGTCTTGGGGCTGAGGTACAGATCTGGTCCTACACGAAGCCCA
GGTACCAGAAAGAGCTCAACCTCACACAGCAGCTGCCTGACTGCAGGGGTCTTGAAGTCCGGGACAGCAT
CCAGAGCTGCTGGGTCCTGCCCTGGCTCAATGTGTCTACAGATGGTGACAATGTCCTTCTGACACTGGAT
GTCTCTGAGGAGCAGGACTTTAGCTTCTTACTGTACCTGCGTCCAGTCCCGGATGCTCTCAAATCCTTGT
GGTACAAAAACCTGACTGGACCTCAGAACATTACTTTAAACCACACAGACCTGGTTCCCTGCCTCTGCAT
TCAGGTGTGGTCGCTAGAGCCAGACTCTGAGAGGGTCGAATTCTGCCCCTTCCGGGAAGATCCCGGTGCA
CACAGGAACCTCTGGCACATAGCCAGGCTGCGGGTACTGTCCCCAGGGGTATGGCAGCTAGATGCGCCTT
GCTGTCTGCCGGGCAAGGTAACACTGTGCTGGCAGGCACCAGACCAGAGTCCCTGCCAGCCACTTGTGCC
ACCAGTGCCCCAGAAGAACGCCACTGTGAATGAGCCACAAGATTTCCAGTTGGTGGCAGGCCACCCCAAC
CTCTGTGTCCAGGTGAGCACCTGGGAGAAGGTTCAGCTGCAAGCGTGCTTGTGGGCTGACTCCTTGGGGC
CCTTCAAGGATGATATGCTGTTAGTGGAGATGAAAACCGGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3154616), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13379. Forward Primer - name:013379_F_IRAV01-03_A02_Il17rc, sequence:TGTGTCCGTGTGGTGGTC; Reverse Primer - name:013379_R_SP6_IRAV01-03_A02_Il17rc, sequence:AGGCCGGTTTTCATCTCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18166 same embryo
 EMAGE:18168 same embryo
 EMAGE:18165 same embryo
 EurExpress:euxassay_011899 same experiment
 MGI:4825569 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS