EMAGE mouse gene expression spatial database

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EMAGE:18168

Fcgrt Fc receptor, IgG, alpha chain transporter ( MGI:103017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011956_01	euxassay_011956_02	euxassay_011956_03	euxassay_011956_04

euxassay_011956_05	euxassay_011956_06	euxassay_011956_07	euxassay_011956_08	euxassay_011956_09

euxassay_011956_10	euxassay_011956_11	euxassay_011956_12	euxassay_011956_13	euxassay_011956_14

euxassay_011956_15	euxassay_011956_16	euxassay_011956_17	euxassay_011956_18	euxassay_011956_19

euxassay_011956_20	euxassay_011956_21	euxassay_011956_22	euxassay_011956_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18168_wholemount_strong.wlz

18168_wholemount_moderate.wlz

18168_wholemount_weak.wlz

18168_wholemount_possible.wlz

18168_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18168_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 18 19 20 21 22

fibula

moderate

homogeneous

moderate expression: see section 02 03

tibia

moderate

homogeneous

moderate expression: see section 01 02 03 04 05

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

otic capsule

moderate

homogeneous

moderate expression: see section 06 07 08 14 15

naris

moderate

homogeneous

moderate expression: see section 13 14 17

nasal septum

moderate

homogeneous

moderate expression: see section 14

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 21

axial skeleton

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 16 17 18

basisphenoid bone

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 19 20 21

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 05 06 07 08 19 20

scapula

moderate

homogeneous

moderate expression: see section 01 02 03 18 19

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 08 09 10 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30039
Entity Detected:	Fcgrt, Fc receptor, IgG, alpha chain transporter ( MGI:103017)
Sequence:	sense strand is shown >T30039 CATCTACGGGGCTTCCCTCTTTCTGGGCCACAGGCTGGTTGGGTCCTCAGCAGTATCTGACCTACAACAG CCTGCGGCAGGAAGCTGACCCCTGTGGGGCCTGGATGTGGGAAAATCAGGTGTCTTGGTATTGGGAGAAG GAGACCACAGACCTCAAAAGCAAAGAACAGCTCTTCTTGGAGGCCCTCAAGACCCTGGAGAAGATATTAA ATGGTCAGAAGAGGGGGACCTACACACTGCAGGGCCTGCTGGGCTGTGAACTGGCCTCGGATAATTCCTC AGTGCCCACGGCTGTGTTTGCCCTCAATGGTGAGGAGTTTATGAAATTCAACCCAAGAATCGGCAATTGG ACTGGGGAGTGGCCTGAGACGGAAATCGTTGCTAATCTGTGGATGAAGCAGCCTGATGCGGCCAGGAAGG AGAGCGAGTTCCTGCTAAACTCTTGTCCGGAGCGACTGCTAGGCCACCTGGAGAGGGGCCGACGGAACCT GGAGTGGAAGGAGCCGCCGTCTATGCGCCTGAAGGCCCGTCCTGGCAACTCTGGCTCCTCCGTGCTGACC TGTGCTGCTTTCTCCTTCTACCCACCGGAGCTCAAGTTCCGATTCCTGCGCAATGGGCTAGCCTCAGGCT CCGGGAATTGCAGCACTGGTCCCAATGGAGATGGCTCTTTCCACGCATGGTCATTGCTGGAGGTCAAACG TGGAGATGAGCACCATTATCAATGTCAAGTGGAGCATGAGGGGCTGGCACAGCCTCTCACTGTGGACCTA GATTCATCAGCCAGATCTTCTGTGCCTGTGGTTGGAATCGTTCTTGGTTTATTGCTGGTGGTAGTGGCCA TCGCAGGCGGTGTGCTGCTGTGGGGCAGGATGCGCAGCGGTCTGCCAGCCCCATGGCTTTCTCTCAGCGG CGATGACTCTGGTGACCTGTTGCCTGGTGGGAACTTGCCCCCAGAAGCTGAACCTCAAGGTGCAAATGCC TTTCCAGCCACTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3485649), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10996. Forward Primer - name:010996_F_IRAV01-03_H15_Fcgrt, sequence:CATCTACGGGGCTTCCCT; Reverse Primer - name:010996_R_SP6_IRAV01-03_H15_Fcgrt, sequence:GAAGTGGCTGGAAAGGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18167 same embryo
	EMAGE:18166 same embryo
	EMAGE:18165 same embryo
	EurExpress:euxassay_011956 same experiment
	MGI:4824817 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source