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EMAGE:18180

Fzr1 fizzy/cell division cycle 20 related 1 (Drosophila) ( MGI:1926790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180
"Pseudo-wholemount" of euxassay_011957. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011957_01 euxassay_011957_02 euxassay_011957_03 euxassay_011957_04
EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180
euxassay_011957_05 euxassay_011957_06 euxassay_011957_07 euxassay_011957_08 euxassay_011957_09
EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180
euxassay_011957_10 euxassay_011957_11 euxassay_011957_12 euxassay_011957_13 euxassay_011957_14
EMAGE:18180 EMAGE:18180 EMAGE:18180 EMAGE:18180
euxassay_011957_15 euxassay_011957_16 euxassay_011957_17 euxassay_011957_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18180Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18180_wholemount_strong.wlz
18180_wholemount_moderate.wlz
18180_wholemount_weak.wlz
18180_wholemount_possible.wlz
18180_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18180_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
homogeneousmoderate expression: see section 01 16 17 18 weak expression: see section 02 03 04 05 06 07 11 12 13 14 15
radius
moderate moderate
homogeneousmoderate expression: see section 18
ulna
moderate moderate
homogeneousmoderate expression: see section 18
humerus
moderate moderate
homogeneousmoderate expression: see section 01 02 14 15 16 17 18
fibula
moderate moderate
homogeneousmoderate expression: see section 02 03 04
tibia
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06
femur
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 16 17 18
otic capsule
moderate moderate
homogeneousmoderate expression: see section 04 05 06 11 12
naris
moderate moderate
homogeneousmoderate expression: see section 07 08 09
nasal septum
moderate moderate
homogeneousmoderate expression: see section 08 09
viscerocranium
moderate moderate
homogeneousExpression in the turbinate bone.
meckel's cartilage
moderate moderate
homogeneousmoderate expression: see section 01 03 04 05 06 07 08 09 10 11 12 13 14
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
axial skeleton
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12
basioccipital bone
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 14 15 16 17 18
basisphenoid bone
moderate moderate
homogeneousmoderate expression: see section 06 07 09 10 11 12
temporal bone petrous part
moderate moderate
homogeneousmoderate expression: see section 01 02 03 14 15 16 17 18
orbito-sphenoid
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 14 15 16 17 18
scapula
moderate moderate
homogeneousmoderate expression: see section 01 02 15
pelvic girdle skeleton
moderate moderate
homogeneousmoderate expression: see section 08 09 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30057
Entity Detected:Fzr1, fizzy/cell division cycle 20 related 1 (Drosophila) ( MGI:1926790)
Sequence:sense strand is shown

>T30057
TCCCCCGTTAGCAACAAAAGTCAGAAGCTGCTGCGGTCACCACGGAAGCCCACACGCAAGATCTCTAAGA
TTCCCTTCAAGGTGCTGGACGCGCCAGAGCTTCAGGACGACTTCTACCTCAACTTGGTGGACTGGTCCTC
CCTCAACGTGCTCAGCGTGGGGCTGGGCACCTGCGTGTACCTGTGGAGTGCATGCACCAGCCAGGTGACC
CGGCTCTGTGACCTCTCTGTAGAAGGGGACTCAGTGACTTCCGTTGGCTGGTCTGAGCGGGGGAACTTGG
TCGCAGTAGGTACACACAAGGGCTTCGTGCAGATCTGGGACGCTGCTGCTGGGAAGAAGCTGTCCATGCT
GGAGGGCCACACAGCACGAGTGGGGGCGCTGGCCTGGAATGCTGACCAGTTGTCATCTGGTAGCCGTGAC
CGCATGATCCTACAGCGGGATATCCGCACACCACCCCTGCAGTCAGAGCGGCGGCTGCAGGGCCACCGGC
AGGAAGTGTGTGGCCTAAAGTGGTCCACAGACCACCAGCTGCTTGCCTCGGGGGGCAATGACAACAAGCT
GCTCGTGTGGAACCACTCTAGTCTAAGCCCTGTGCAGCAGTATACGGAGCACCTGGCAGCCGTGAAGGCT
ATTGCCTGGTCCCCACACCAGCATGGACTGCTGGCATCTGGTGGTGGCACGGCTGACCGCTGTATCCGAT
TCTGGAACACTCTGACAGGTCAGCCACTGCAGTGCATTGACACAGGCTCACAAGTGTGCAACCTGGCCTG
GTCCAAGCACGCCAATGAGCTGGTGAGCACACATGGCTACTCACAGAACCAGATCCTCGTGTGGAAGTAC
CCGTCCCTTACGCAGGTGGCCAAGCTCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3967009), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15895. Forward Primer - name:015895_F_IRAV20-23_A20_Fzr1, sequence:TCCCCCGTTAGCAACAAA; Reverse Primer - name:015895_R_SP6_IRAV20-23_A20_Fzr1, sequence:AGTGAGCTTGGCCACCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18181 same embryo
 EMAGE:18183 same embryo
 EMAGE:18185 same embryo
 EMAGE:18184 same embryo
 EMAGE:18182 same embryo
 EurExpress:euxassay_011957 same experiment
 MGI:4824957 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS